Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104105
Name   oriT_pEA32_1 in_silico
Organism   Escherichia albertii strain BIA_32
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP117601 (109275..109398 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      101..106, 119..124  (TTTAAT..ATTAAA)
 91..99, 113..121  (AATAATGTA..TACATTATT)
 90..95, 107..112  (AAATAA..TTATTT)
 88..93, 105..110  (ATAAAT..ATTTAT)
 57..62, 70..75  (TGATTT..AAATCA)
 41..48, 61..68  (AAAAACAA..TTGTTTTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pEA32_1
GGTTAGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGTTTTTTAAATCATTAGTTTATGTTATAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   3025 GenBank   WP_273823906
Name   traI_PS047_RS24290_pEA32_1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192161.95 Da        Isoelectric Point: 5.7465

>WP_273823906.1 conjugative transfer relaxase/helicase TraI [Escherichia albertii]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGLPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGEMQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGDGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTQELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQEWAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSDEGRVKGSGDAQFVALQGSRNGENLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DSKTEQAVREIAGQERDRSAISEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

DNA helicase TraI (TraI_C)

(1434-1557)

AAA domain (AAA_30)

(969-1155)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-625)

TrwC relaxase (TrwC)

(10-283)


  Protein structure



No available structure.




Auxiliary protein

ID   1295 GenBank   WP_001254386
Name   WP_001254386_pEA32_1 insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5

ID   1296 GenBank   WP_149450799
Name   WP_149450799_pEA32_1 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14489.54 Da        Isoelectric Point: 4.8381

>WP_149450799.1 conjugal transfer relaxosome DNA-binding protein TraM [Escherichia albertii]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMECKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure



No available structure.




T4CP

ID   2909 GenBank   WP_273823907
Name   traD_PS047_RS24295_pEA32_1 insolico UniProt ID   _
Length   717 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 717 a.a.        Molecular weight: 81535.01 Da        Isoelectric Point: 4.9604

>WP_273823907.1 type IV conjugative transfer system coupling protein TraD [Escherichia albertii]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTENSKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQERPKVAPEFIPREINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVN
INVYRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   2910 GenBank   WP_106907105
Name   traC_PS047_RS24380_pEA32_1 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99613.56 Da        Isoelectric Point: 6.6265

>WP_106907105.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQY
TANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
MSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(39-277)

TraG P-loop domain (P-loop_TraG)

(468-772)

Family of unknown function (DUF5934) (DUF5934)

(291-447)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 83108..109970

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PS047_RS24295 (PS047_24295) 83108..85261 - 2154 WP_273823907 type IV conjugative transfer system coupling protein TraD virb4
PS047_RS24300 (PS047_24300) 85514..86245 - 732 WP_000850424 conjugal transfer complement resistance protein TraT -
PS047_RS24305 (PS047_24305) 86259..86768 - 510 WP_000628099 conjugal transfer entry exclusion protein TraS -
PS047_RS24310 (PS047_24310) 86765..89590 - 2826 WP_001007030 conjugal transfer mating-pair stabilization protein TraG traG
PS047_RS24315 (PS047_24315) 89587..90960 - 1374 WP_273823937 conjugal transfer pilus assembly protein TraH traH
PS047_RS24320 (PS047_24320) 90947..91339 - 393 WP_040072248 F-type conjugal transfer protein TrbF -
PS047_RS24325 (PS047_24325) 91326..91606 - 281 Protein_81 P-type conjugative transfer protein TrbJ -
PS047_RS24330 (PS047_24330) 91596..92141 - 546 Protein_82 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
PS047_RS24335 (PS047_24335) 92128..92412 - 285 WP_000624109 type-F conjugative transfer system pilin chaperone TraQ -
PS047_RS24340 (PS047_24340) 92531..92878 - 348 WP_114011940 conjugal transfer protein TrbA -
PS047_RS24345 (PS047_24345) 92894..93637 - 744 WP_059219324 type-F conjugative transfer system pilin assembly protein TraF traF
PS047_RS24350 (PS047_24350) 93630..93887 - 258 WP_273823938 conjugal transfer protein TrbE -
PS047_RS24355 (PS047_24355) 93914..95722 - 1809 WP_273823908 type-F conjugative transfer system mating-pair stabilization protein TraN traN
PS047_RS24360 (PS047_24360) 95719..96357 - 639 WP_000777692 type-F conjugative transfer system pilin assembly protein TrbC trbC
PS047_RS24365 (PS047_24365) 96366..97358 - 993 WP_000830839 conjugal transfer pilus assembly protein TraU traU
PS047_RS24370 (PS047_24370) 97355..97987 - 633 WP_001203720 type-F conjugative transfer system protein TraW traW
PS047_RS24375 (PS047_24375) 97984..98370 - 387 WP_000214098 type-F conjugative transfer system protein TrbI -
PS047_RS24380 (PS047_24380) 98367..100997 - 2631 WP_106907105 type IV secretion system protein TraC virb4
PS047_RS24385 (PS047_24385) 101123..101485 - 363 WP_029400175 hypothetical protein -
PS047_RS24390 (PS047_24390) 101513..101731 - 219 WP_000556751 hypothetical protein -
PS047_RS24395 (PS047_24395) 101811..102284 - 474 WP_114011942 hypothetical protein -
PS047_RS24400 (PS047_24400) 102277..102498 - 222 WP_001278978 conjugal transfer protein TraR -
PS047_RS24405 (PS047_24405) 102633..103148 - 516 WP_000809874 type IV conjugative transfer system lipoprotein TraV traV
PS047_RS24410 (PS047_24410) 103145..103465 - 321 WP_001057305 conjugal transfer protein TrbD -
PS047_RS24415 (PS047_24415) 103452..104042 - 591 WP_000002784 conjugal transfer pilus-stabilizing protein TraP -
PS047_RS24420 (PS047_24420) 104032..105459 - 1428 WP_273823909 F-type conjugal transfer pilus assembly protein TraB traB
PS047_RS24425 (PS047_24425) 105459..106187 - 729 WP_273823910 type-F conjugative transfer system secretin TraK traK
PS047_RS24430 (PS047_24430) 106174..106740 - 567 WP_273823911 type IV conjugative transfer system protein TraE traE
PS047_RS24435 (PS047_24435) 106762..107073 - 312 WP_000012118 type IV conjugative transfer system protein TraL traL
PS047_RS24440 (PS047_24440) 107088..107453 - 366 WP_273823912 type IV conjugative transfer system pilin TraA -
PS047_RS24445 (PS047_24445) 107487..107714 - 228 WP_001254386 conjugal transfer relaxosome protein TraY -
PS047_RS24450 (PS047_24450) 107808..108494 - 687 WP_273823913 PAS domain-containing protein -
PS047_RS24455 (PS047_24455) 108685..109068 - 384 WP_149450799 conjugal transfer relaxosome DNA-binding protein TraM -
PS047_RS24460 (PS047_24460) 109368..109970 + 603 WP_000243701 transglycosylase SLT domain-containing protein -
PS047_RS24465 (PS047_24465) 110266..111087 - 822 Protein_109 DUF932 domain-containing protein -
PS047_RS24470 (PS047_24470) 111209..111495 - 287 Protein_110 hypothetical protein -
PS047_RS26045 111520..111681 - 162 WP_337961363 single-stranded DNA-binding protein -
PS047_RS24475 (PS047_24475) 111796..112222 + 427 Protein_112 hypothetical protein -
PS047_RS24480 (PS047_24480) 112305..112871 - 567 WP_273823914 class I SAM-dependent methyltransferase -
PS047_RS24485 (PS047_24485) 112920..114281 - 1362 WP_273823915 DUF3560 domain-containing protein -
PS047_RS24490 (PS047_24490) 114333..114563 - 231 WP_000218637 hypothetical protein -

Host bacterium

ID   4544 GenBank   NZ_CP117601
Plasmid name   pEA32_1 Incompatibility group   IncFIB
Plasmid size   125136 bp Coordinate of oriT [Strand]   109275..109398 [-]
Host baterium   Escherichia albertii strain BIA_32

Cargo genes

Drug resistance gene   -
Virulence gene   espL2, gspE, gspD, gspC
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -