Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104100 |
| Name | oriT_pEA5_1  |
| Organism | Escherichia albertii strain BIA_5 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP117576 (134948..135297 [+], 350 nt) |
| oriT length | 350 nt |
| IRs (inverted repeats) | 190..196, 204..210 (TATAAAA..TTTTATA) 137..143, 157..163 (GCGGTGT..ACACCGC) 38..45, 48..55 (GCAAAAAC..GTTTTTGC) 2..7, 16..21 (TTGGTG..CACCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 350 nt
>oriT_pEA5_1
GTTGGTGTTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGTGTTGTAGCCGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGCGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTTAAAAAAGGTAATCTCTA
GTTGGTGTTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGTGTTGTAGCCGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGCGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCATTATGAATTTTAGTGTTTCGAAATTAACTTTATTTTATGTTTAAAAAAGGTAATCTCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Auxiliary protein
| ID | 1291 | GenBank | WP_273821939 |
| Name | WP_273821939_pEA5_1 |
UniProt ID | _ |
| Length | 127 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14644.62 Da Isoelectric Point: 5.0734
>WP_273821939.1 conjugal transfer relaxosome DNA-binding protein TraM [Escherichia albertii]
MAKVNLYIRNDAYEKINAIIEKRRQEGEREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE
MAKVNLYIRNDAYEKINAIIEKRRQEGEREKDVSFSATASMLLELGLRVHEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 1292 | GenBank | WP_273821940 |
| Name | WP_273821940_pEA5_1 |
UniProt ID | _ |
| Length | 106 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 106 a.a. Molecular weight: 12026.95 Da Isoelectric Point: 10.7023
>WP_273821940.1 TraY domain-containing protein [Escherichia albertii]
MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRCRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVKYL
TMRLDDETNQLLIAAKNRSGWCKTDGAADRVIDQVS
MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRCRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVKYL
TMRLDDETNQLLIAAKNRSGWCKTDGAADRVIDQVS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 4539 | GenBank | NZ_CP117576 |
| Plasmid name | pEA5_1 | Incompatibility group | IncFIB |
| Plasmid size | 143188 bp | Coordinate of oriT [Strand] | 134948..135297 [+] |
| Host baterium | Escherichia albertii strain BIA_5 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | hsiC1/vipB, hsiB1/vipA, faeJ, faeI, faeH, faeF, faeE, faeC |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |