oriTDB

Detailed information of oriT

oriT
Relaxase
Auxiliary protein
T4CP
T4SS
Host bacterium
Cargo genes

oriT

The information of the oriT region

oriTDB ID	104083
Name	oriT_pEA25_2
Organism	Escherichia albertii strain BIA_25
Sequence Completeness	-
*NCBI accession of oriT* (coordinates [strand])**	NZ_CP117617 (56864..57215 [+], 352 nt)
oriT length	352 nt
IRs (inverted repeats)	260..268, 279..287 (CAGGGGCGC..GCGCCCCTG) 192..198, 206..212 (TATAAAA..TTTTATA) 104..110, 118..124 (TTTAAAT..ATTTAAA) 40..47, 50..57 (GCAAAAAC..GTTTTTGC) 4..11, 16..23 (TTGGTGGT..ACCACCAA)
*Location of nic* site**	_
Conserved sequence flanking the nic site	_
Note	Predicted by oriTfinder 2.0

oriT sequence

Download Length: 352 nt

                        >oriT_pEA25_2
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATTTGATTTATTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGCCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA                      

Visualization of oriT structure

oriT secondary structure

Predicted by RNAfold.

Download structure file

Relaxase

traI_PS046_RS24355_pEA25_2

ID	3019	GenBank	WP_273813378
Name	traI_PS046_RS24355_pEA25_2	UniProt ID	_
Length	1756 a.a.	PDB ID
Note	Predicted by oriTfinder 2.0

Relaxase protein sequence

Download Length: 1756 a.a. Molecular weight: 192268.92 Da Isoelectric Point: 6.1461

          >WP_273813378.1 conjugative transfer relaxase/helicase TraI [Escherichia albertii]

MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ

DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV

MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQITFGRLYREKLKEQVEA

LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTEWLQT

LKETGFDIRAYRDAADQRAEIRTQTPGPASQDWPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPG

NGVIDRARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVAVHPEKSVPRTACYS

DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR

RQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW

QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHQEVT

MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS

SWSLFRPEKMPVADGERLRVTGKIPGLRVYGGDRLQAASVSEDAMTVVVPGRAEPATLPVADSPFTALKL

ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDEPRTAEKLARHPSFTVVSEQI

KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDA

QIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI

LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT

QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA

ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPERSVTEFSHSQEAKLAEA

QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM

VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT

LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIHPGQERAEQHIDLAYAI

TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF

EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG

IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE

GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP

DGKTEQAVREIAGQEHDRSAISEREAALPESVLREPQRVREAVREIARENLLQERLQQMERDMVRDLQKE

KTLGGD

Protein domains

Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(969-1156)

DNA helicase TraI (TraI_C)

(1434-1557)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-627)

TrwC relaxase (TrwC)

(10-283)

Protein structure

No available structure.

Auxiliary protein

WP_001151530_pEA25_2

ID	1284	GenBank	WP_001151530
Name	WP_001151530_pEA25_2	UniProt ID	A0A385JKG6
Length	127 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

Auxiliary protein sequence

Download Length: 127 a.a. Molecular weight: 14525.54 Da Isoelectric Point: 4.8662

                        >WP_001151530.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]

MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL

LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE

Protein domains

Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)

Protein structure

Source	ID	Structure
AlphaFold DB	A0A385JKG6

ID	2898	GenBank	WP_181668576
Name	traC_PS046_RS24235_pEA25_2	UniProt ID	_
Length	875 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 875 a.a. Molecular weight: 99144.73 Da Isoelectric Point: 5.7326

                        >WP_181668576.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]

MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP

INGANESIVEALDHMLRTKLPRGVPFCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAA

TQFPLPEGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVG

EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL

WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE

LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG

LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA

GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV

MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT

AGGTYGRYFNSDEPSLQDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR

LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN

QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL

SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

Protein domains

Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(467-771)

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraC protein (TraC_F_IV)

(38-276)

Protein structure

No available structure.

ID	2899	GenBank	WP_273813376
Name	traD_PS046_RS24350_pEA25_2	UniProt ID	_
Length	717 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 717 a.a. Molecular weight: 81482.15 Da Isoelectric Point: 5.1711

                        >WP_273813376.1 type IV conjugative transfer system coupling protein TraD [Escherichia albertii]

MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR

DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ

QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR

KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG

SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKA

IRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV

WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA

EFAAGEIGEKEHLKASLQYSYGADPVRDGISTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL

KYQARPKIAPEFIPREINPEMENRLSAVLAAREAEGRQVASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI

NDKKSDAGVNVPAGGIEPELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVN

INVHRERGEDVEPGDDF

Protein domains

Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 56293..79762

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PS046_RS24135 (PS046_24135)	51352..51585	+	234	WP_071588969	hypothetical protein	-
PS046_RS24140 (PS046_24140)	51826..52086	+	261	WP_042046747	hypothetical protein	-
PS046_RS24145 (PS046_24145)	52203..53189	-	987	WP_273810655	IS110 family transposase	-
PS046_RS24155 (PS046_24155)	54770..55057	+	288	WP_000107535	hypothetical protein	-
PS046_RS24160 (PS046_24160)	55175..55996	+	822	WP_001234426	DUF932 domain-containing protein	-
PS046_RS24165 (PS046_24165)	56293..56940	-	648	WP_155953419	transglycosylase SLT domain-containing protein	virB1
PS046_RS24170 (PS046_24170)	57216..57599	+	384	WP_001151530	conjugal transfer relaxosome DNA-binding protein TraM	-
PS046_RS24175 (PS046_24175)	57791..58438	+	648	WP_096040777	transcriptional regulator TraJ family protein	-
PS046_RS24180 (PS046_24180)	58558..58785	+	228	WP_000589558	conjugal transfer relaxosome protein TraY	-
PS046_RS24185 (PS046_24185)	58819..59211	+	393	WP_024127870	type IV conjugative transfer system pilin TraA	-
PS046_RS24190 (PS046_24190)	59226..59537	+	312	WP_001585108	type IV conjugative transfer system protein TraL	traL
PS046_RS24195 (PS046_24195)	59559..60125	+	567	WP_059259957	type IV conjugative transfer system protein TraE	traE
PS046_RS24200 (PS046_24200)	60138..60839	+	702	WP_273813362	type-F conjugative transfer system secretin TraK	traK
PS046_RS24205 (PS046_24205)	60839..62266	+	1428	WP_273813366	F-type conjugal transfer pilus assembly protein TraB	traB
PS046_RS24210 (PS046_24210)	62256..62846	+	591	WP_001376242	conjugal transfer pilus-stabilizing protein TraP	-
PS046_RS24215 (PS046_24215)	62833..63030	+	198	WP_001324648	conjugal transfer protein TrbD	-
PS046_RS24220 (PS046_24220)	63043..63294	+	252	WP_001038341	conjugal transfer protein TrbG	-
PS046_RS24225 (PS046_24225)	63291..63806	+	516	WP_016241856	type IV conjugative transfer system lipoprotein TraV	traV
PS046_RS24230 (PS046_24230)	63941..64162	+	222	WP_001278685	conjugal transfer protein TraR	-
PS046_RS24235 (PS046_24235)	64322..66949	+	2628	WP_181668576	type IV secretion system protein TraC	virb4
PS046_RS24240 (PS046_24240)	66946..67332	+	387	WP_181668575	type-F conjugative transfer system protein TrbI	-
PS046_RS24245 (PS046_24245)	67329..67961	+	633	WP_001203749	type-F conjugative transfer system protein TraW	traW
PS046_RS24250 (PS046_24250)	67958..68950	+	993	WP_000830839	conjugal transfer pilus assembly protein TraU	traU
PS046_RS24255 (PS046_24255)	68976..69440	+	465	WP_096948751	hypothetical protein	-
PS046_RS24260 (PS046_24260)	69451..69766	+	316	Protein_91	hypothetical protein	-
PS046_RS24265 (PS046_24265)	69759..70211	+	453	WP_001349502	hypothetical protein	-
PS046_RS24270 (PS046_24270)	70239..70877	+	639	WP_273810593	type-F conjugative transfer system pilin assembly protein TrbC	trbC
PS046_RS24275 (PS046_24275)	70874..72682	+	1809	WP_042973378	type-F conjugative transfer system mating-pair stabilization protein TraN	traN
PS046_RS24280 (PS046_24280)	72709..72966	+	258	WP_000864318	conjugal transfer protein TrbE	-
PS046_RS24285 (PS046_24285)	72959..73702	+	744	WP_001030378	type-F conjugative transfer system pilin assembly protein TraF	traF
PS046_RS24290 (PS046_24290)	73718..74065	+	348	WP_042973381	conjugal transfer protein TrbA	-
PS046_RS24295 (PS046_24295)	74067..74381	-	315	WP_000415566	protein ArtA	-
PS046_RS24300 (PS046_24300)	74462..74746	+	285	WP_000624108	type-F conjugative transfer system pilin chaperone TraQ	-
PS046_RS24305 (PS046_24305)	74733..75278	+	546	WP_000059833	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	traF
PS046_RS24310 (PS046_24310)	75208..75570	+	363	WP_174491168	P-type conjugative transfer protein TrbJ	-
PS046_RS24315 (PS046_24315)	75570..76943	+	1374	WP_001137358	conjugal transfer pilus assembly protein TraH	traH
PS046_RS24320 (PS046_24320)	76940..79762	+	2823	WP_001007026	conjugal transfer mating-pair stabilization protein TraG	traG
PS046_RS24325 (PS046_24325)	79777..80202	+	426	WP_273813374	surface exclusion protein	-
PS046_RS24330 (PS046_24330)	80317..81858	+	1542	WP_001298859	IS21-like element ISEc12 family transposase	-
PS046_RS24335 (PS046_24335)	81873..82619	+	747	WP_001016257	IS21-like element ISEc12 family helper ATPase IstB	-
PS046_RS24340 (PS046_24340)	82711..82866	+	156	Protein_107	surface exclusion protein	-
PS046_RS24345 (PS046_24345)	82898..83629	+	732	WP_000850424	conjugal transfer complement resistance protein TraT	-

Host bacterium

ID	4522	GenBank	NZ_CP117617
Plasmid name	pEA25_2	Incompatibility group	-
Plasmid size	96063 bp	Coordinate of oriT [Strand]	56864..57215 [+]
Host baterium	Escherichia albertii strain BIA_25

Cargo genes

Drug resistance gene	-
Virulence gene	-
Metal resistance gene	-
Degradation gene	-
Symbiosis gene	-
Anti-CRISPR	-