Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   104041
Name   oriT_H110|unnamed in_silico
Organism   Proteus mirabilis strain H110
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OL790812 (3443550..3443848 [-], 299 nt)
oriT length   299 nt
IRs (inverted repeats)      234..242, 254..262  (AAAGCCAAA..TTTGGCTTT)
 45..50, 52..57  (CAAACG..CGTTTG)
 7..12, 26..31  (TTTGGC..GCCAAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 299 nt

>oriT_H110|unnamed
GCTCTGTTTGGCGGCTGGTGACCTAGCCAAAAAAATTGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCAGCCTGTATCCCTTGTCGGGTATGGGATTGAGCGAGTCGATTTATATCGAGACGCCAAACAGTGATTGTGACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTAGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2996 GenBank   WP_064497046
Name   mobH_MCX88_RS16030_H110|unnamed insolico UniProt ID   A0A0U2I2S1
Length   716 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 716 a.a.        Molecular weight: 79760.84 Da        Isoelectric Point: 5.2184

>WP_064497046.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MFKNLFFQAKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTSNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWIRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLAMLHIVEADLLFSSNVPSSVRLFSKSEWEATPQTQAEPQSRSSEQPGL
PDASSSIEHGNSSESPSTKSSDQDDELRLASDVNNPQANENAPGDGCENPNNSYDGAISNNVNQHDAEAF
NLPESLAWLPEASSALVMVDEQIMIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTKDGV
QEQGLLLKVSISKGLTALIDLPKQDAEPAAAIQNEEALQRPSRTKTTNAQAKEPATRAERKQKPIGPNAN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPYVDHSADGIRVTIQTLRQVAKEHGIPAGQLLRGISASDEC
QFDEGETVLFTARAER

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(39-349)


  Protein structure


Source ID Structure
AlphaFold DB A0A0U2I2S1


T4CP

ID   2865 GenBank   WP_237737483
Name   tcpA_MCX88_RS06320_H110|unnamed insolico UniProt ID   _
Length   1299 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 1299 a.a.        Molecular weight: 143860.71 Da        Isoelectric Point: 4.3399

>WP_237737483.1 DNA translocase FtsK 4TM domain-containing protein [Proteus mirabilis]
MSQEYTEDKNIRFTKISNRRRIWEALLILIGIGAIFLMISLLSFHPADPSWSQTTWNEPIQNLGGNIGAW
LADILFSAFGLLAYAIPIVVVFGCWNALRHQKNREYTDFFSLALRTIGALALVLTSCALADLNFDDIYNF
SSGGVIGSLFSKALLPWFNMLGATLALLSVWAIGFTLFTGWSWLTITEKIGAVILGAVAFITNRGQKDID
YDDYEEPADPIQADPEVLVDNNTQPEHQLSAQIEPDSDDILFSAPSALELARQQEMAITPQHDDITETSE
VASGSATPTISATETVIAEAHESKERAEHIVDNQQPTHATTHDAEVTPPSIMATHDASPVTEPEPEHYRF
EIPSAFLKQQAHSSDSMADNSVVTEEVPTTMPLTENKPIETNEASIVSQKTAADNSDHSASLFTPSATST
DSNSASLTQTAAGVASVATAAIVSHGAQVKQGLGPELPRPNPVRLPTRRELYGIRIPSQREAELRRREEA
LQQDHEDDLADIQEQANFEQSLRQQFLEQQRERYGEDTSATQNVEPTITMSSATSSSQSTYDEYDESDNQ
PFIDTHTFSAQETEEESDEYEQHLAEQFKQQLQERYGEDVLQDEDDEVLPSVTTGASETSASAQGYHPHI
QHSFASQSTPVTQQTDNSTLYPNSDAFLQVSPRDDEEEEYTPTIELQENLDALGSLSPVDDLLDEEPAEP
VFTPMVTPAEPVSSVSSVGQTPIEPQAQMAQHFAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ
QQQQQQQQQQQQQQQQQSLIHPLLMRNDRPLQKPTTPMPSLDLLTSPPMDDEPVDMFELERIGKLIEARL
NDYRVKAKVVGISPGPVITRFELELAPGVKAARISNLSRDLARSLSTTAVRIVEVIPGKPYVGLELPNKK
RQTVYMRELLDSDAFRDSRSPLTVVLGKDIGGQPVVANLAKMPHLLVAGTTGSGKSVGVNAMIISILYKA
KPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAASALRWSVAEMERRYKLMSALGVRNLAGYNEKI
KEAEAMGRPIPDPLWKPSDSMAPELPMLEKEPYIVVIVDEFADLMMTAGKKVEELIARLAQKARAAGIHL
VLATQRPSVDIITGLIKANIPSRIAFTVSSKIDSRTILDQGGAESLLGMGDMLYAPNGYMPERVHGAFVS
DDEVHAVATDWKARGRPQYIEAITKCGEEGEGGNGGGYDDGEELDPLFDQAVEFVVEKQRVSISGVQRQF
RIGYNRAARIVEQMEMQGIVSAPNHNNTRDVLSPPPAEM

  Protein domains


Predicted by InterproScan.

FtsK alpha domain (FtsK_alpha)

(808-908)

4TM region of DNA translocase FtsK/SpoIIIE (FtsK_4TM)

(20-191)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(916-1129)

Ftsk gamma domain (FtsK_gamma)

(1233-1292)

  Protein structure



No available structure.



ID   2866 GenBank   WP_017627952
Name   tcpA_MCX88_RS13105_H110|unnamed insolico UniProt ID   _
Length   478 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 478 a.a.        Molecular weight: 53586.25 Da        Isoelectric Point: 4.9818

>WP_017627952.1 MULTISPECIES: DNA translocase FtsK [Proteus]
MAQPFIHTSELCWSSQELDLMAQAIESCFLQYQIRGEVVGYDEGATFTLFRIELGRGIKVSQVIALVPEL
CRSLSVVDIKVIDFIAGTPYIGLRVTNTYRRAVPFIECFNQWNENNGLSSLSVMLGEDIIGEPIGWDLAQ
MPHLLITGVTRSGKSMLMHSLVMSILYRNPPDKVRFVMFDTSQLELSLYNDIPHLLFPVASDSIESIKPL
SFLVSELQRRQKLFSALNQRNLSGYNKVISNAKELGKPIPDPFGRSNKNYSEHPYLDSEPEIVVCVDDYV
QFIGEYKQIGEMLVLLSQQGHAVGIHLILTTRSPLSTGIGSQLRINIPTRIALSVSSRADSNLILGQYGA
ESLFGLGDMLFVSPSFSGPIRIQGTYVSDSDIRDAVDYCKRWGSVSYLNLYDDVQNTNMSAEELDSLFAQ
VVEFTVEKQWVSISGIHRQFRIGYNRAARIIEQLELQGIVSEQNCNGNREVLAPRFDS

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(119-342)

Ftsk gamma domain (FtsK_gamma)

(413-473)

FtsK alpha domain (FtsK_alpha)

(14-97)

  Protein structure



No available structure.



ID   2867 GenBank   WP_064497039
Name   traC_MCX88_RS15955_H110|unnamed insolico UniProt ID   _
Length   799 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 799 a.a.        Molecular weight: 90171.75 Da        Isoelectric Point: 5.9727

>WP_064497039.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTDRELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYRVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHIIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(436-521)

TraG P-loop domain (P-loop_TraG)

(566-794)

Family of unknown function (DUF5934) (DUF5934)

(253-420)

TraC protein (TraC_F_IV)

(11-241)

  Protein structure



No available structure.



ID   2868 GenBank   WP_064497045
Name   traD_MCX88_RS16025_H110|unnamed insolico UniProt ID   _
Length   606 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 606 a.a.        Molecular weight: 67102.94 Da        Isoelectric Point: 6.2511

>WP_064497045.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYLLYKLQMG
LKGKPLAFMELSHLQKVMAKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIAAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKTNTLAKQAMAMLLFYYERIQPIAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVDNRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FAARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPKTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAA

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(152-277)

TraM recognition site of TraD and TraG (TraG-D_C)

(451-578)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3388483..3413304

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCX88_RS15895 3384134..3384541 - 408 WP_053409935 Cd(II)/Pb(II)-responsive transcriptional regulator -
MCX88_RS15900 3384637..3385533 + 897 WP_071192896 cation transporter -
MCX88_RS15905 3385537..3386049 + 513 WP_004863699 signal peptidase II -
MCX88_RS15910 3386071..3387358 + 1288 Protein_3084 ISL3 family transposase -
MCX88_RS15915 3387400..3388074 + 675 WP_048608563 endonuclease -
MCX88_RS15920 3388483..3390522 - 2040 WP_004249372 DUF87 domain-containing protein virb4
MCX88_RS15925 3390519..3391937 - 1419 WP_004249371 SIR2 family protein -
MCX88_RS15930 3392396..3396088 - 3693 WP_237737462 conjugal transfer protein TraN traN
MCX88_RS15935 3396091..3397119 - 1029 WP_000735887 TraU family protein traU
MCX88_RS15940 3397103..3398227 - 1125 WP_064497037 TrbC family F-type conjugative pilus assembly protein traW
MCX88_RS15945 3398238..3398750 - 513 WP_175215521 S26 family signal peptidase -
MCX88_RS15950 3398734..3399081 - 348 WP_064497038 hypothetical protein -
MCX88_RS15955 3399074..3401473 - 2400 WP_064497039 type IV secretion system protein TraC virb4
MCX88_RS15960 3401473..3402165 - 693 WP_001228924 DsbC family protein trbB
MCX88_RS15965 3402297..3403235 - 939 WP_064497040 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
MCX88_RS15970 3403228..3404019 - 792 WP_000095577 type IV toxin-antitoxin system AbiEi family antitoxin -
MCX88_RS15975 3404240..3404626 - 387 WP_032469918 TraA family conjugative transfer protein -
MCX88_RS15980 3404623..3405195 - 573 WP_024008834 type IV conjugative transfer system lipoprotein TraV traV
MCX88_RS15985 3405270..3406559 - 1290 WP_064497041 TraB/VirB10 family protein traB
MCX88_RS15990 3406562..3407458 - 897 WP_064497055 type-F conjugative transfer system secretin TraK traK
MCX88_RS15995 3407442..3408068 - 627 WP_064497042 TraE/TraK family type IV conjugative transfer system protein traE
MCX88_RS16000 3408065..3408346 - 282 WP_000433891 type IV conjugative transfer system protein TraL traL
MCX88_RS16005 3408483..3409589 - 1107 WP_031500457 XRE family transcriptional regulator -
MCX88_RS16010 3409582..3410253 - 672 WP_124724881 hypothetical protein -
MCX88_RS16015 3410292..3410927 - 636 WP_064497043 DUF4400 domain-containing protein tfc7
MCX88_RS16020 3410914..3411474 - 561 WP_064497044 hypothetical protein -
MCX88_RS16025 3411484..3413304 - 1821 WP_064497045 conjugative transfer system coupling protein TraD virb4
MCX88_RS16030 3413353..3415503 - 2151 WP_064497046 MobH family relaxase -
MCX88_RS16035 3415660..3416841 - 1182 WP_047109294 DUF4268 domain-containing protein -

Host bacterium

ID   4480 GenBank   NZ_OL790812
Plasmid name   H110|unnamed Incompatibility group   -
Plasmid size   4088254 bp Coordinate of oriT [Strand]   3443550..3443848 [-]
Host baterium   Proteus mirabilis strain H110

Cargo genes

Drug resistance gene   cat, tet(J), aadA2, ere(A), dfrA32, sul1, qacE, ARR-3, catB3, blaOXA-1, aac(6')-Ib-cr, aac(3)-IVa, aph(4)-Ia, sul2, floR, blaCTX-M-65, aph(3')-Ia, tet(C), aph(3'')-Ib, aph(6)-Id, dfrA1
Virulence gene   rfaD, ugd, tufA, wbtL, ureG, acrB, gmhA/lpcA, luxS, gnd, allD, msbA, nueA, clpB, AHA_1833, tssB, hcp, ompA, flmH, kdsA, sodB, galU, fliA, fliG, fliI, fliM, fliN, fliP, fliQ, flgI, flgH, flgG, flgC, flgB, flhA, cheZ, cheY, cheB, cheW, cheA, motA, flhC, flhD, rcsB, katA, algU, lpxC, papD, lpxA, rfaE, htpB, rpe
Metal resistance gene   cpxA, cpxR, oxyRkp, zntR/yhdM, acrD/yffA, corB, corC, modE, modB, phoB, yfeC, yfeB, yfeA, ruvB, znuB/yebI, znuC/yebM, kpnE, kpnF, mdtB, mdtC/yegO, actP, arsB, cueR/ybbI, copA, corD, terE, terD, terC, terB, terA, terZ, pstS, pstC, pstA, pstB, actP/yjcG
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7, AcrVA2, AcrVIB, AcrIIC1