Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104040 |
| Name | oriT_kpn-241|unnamed4  |
| Organism | Klebsiella pneumoniae strain kpn-241 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP053669 (10770..10875 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_kpn-241|unnamed4
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2995 | GenBank | WP_050868819 |
| Name | Relaxase_HPK15_RS27230_kpn-241|unnamed4 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 2864 | GenBank | WP_011091092 |
| Name | t4cp2_HPK15_RS27390_kpn-241|unnamed4 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 13671..32774
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HPK15_RS27210 (HPK15_27675) | 8755..9066 | + | 312 | WP_011091065 | hypothetical protein | - |
| HPK15_RS27215 (HPK15_27680) | 9199..9735 | + | 537 | WP_011091066 | hypothetical protein | - |
| HPK15_RS27220 (HPK15_27685) | 10236..10601 | - | 366 | WP_011091067 | hypothetical protein | - |
| HPK15_RS27225 | 10616..11194 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| HPK15_RS27230 (HPK15_27695) | 11181..13160 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| HPK15_RS27235 (HPK15_27700) | 13174..13674 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| HPK15_RS27240 (HPK15_27705) | 13671..14450 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| HPK15_RS27245 (HPK15_27710) | 14461..15624 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| HPK15_RS27250 (HPK15_27715) | 15614..15874 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| HPK15_RS27255 | 15899..19111 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| HPK15_RS27260 (HPK15_27725) | 19077..19589 | + | 513 | WP_011091071 | hypothetical protein | traL |
| HPK15_RS27265 (HPK15_27730) | 19590..19802 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| HPK15_RS27270 (HPK15_27735) | 19774..20184 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| HPK15_RS27275 (HPK15_27740) | 20252..20965 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| HPK15_RS27280 (HPK15_27745) | 20974..22125 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| HPK15_RS27285 (HPK15_27750) | 22137..23486 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| HPK15_RS27290 (HPK15_27755) | 23498..24202 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| HPK15_RS27295 (HPK15_27760) | 24226..24756 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| HPK15_RS27300 (HPK15_27765) | 24773..25162 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| HPK15_RS27305 (HPK15_27770) | 25208..25702 | + | 495 | WP_011091080 | hypothetical protein | - |
| HPK15_RS27310 (HPK15_27775) | 25699..28749 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| HPK15_RS27315 (HPK15_27780) | 28746..29954 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| HPK15_RS27320 (HPK15_27785) | 29951..30601 | + | 651 | WP_011091083 | hypothetical protein | - |
| HPK15_RS27325 (HPK15_27790) | 30594..32774 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| HPK15_RS27330 (HPK15_27795) | 32777..33430 | + | 654 | WP_011091084 | hypothetical protein | - |
| HPK15_RS27335 (HPK15_27800) | 33505..33735 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| HPK15_RS27340 (HPK15_27805) | 34033..35088 | + | 1056 | WP_011091086 | plasmid replication initiator RepA | - |
| HPK15_RS27345 (HPK15_27810) | 36092..36952 | - | 861 | WP_000027057 | broad-spectrum class A beta-lactamase TEM-1 | - |
Host bacterium
| ID | 4479 | GenBank | NZ_CP053669 |
| Plasmid name | kpn-241|unnamed4 | Incompatibility group | IncL/M |
| Plasmid size | 71962 bp | Coordinate of oriT [Strand] | 10770..10875 [+] |
| Host baterium | Klebsiella pneumoniae strain kpn-241 |
Cargo genes
| Drug resistance gene | blaTEM-1B, blaCTX-M-15 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |