Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 104035 |
| Name | oriT_pAK76  |
| Organism | Sinorhizobium meliloti strain AK76 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP066360 (50512..50566 [-], 55 nt) |
| oriT length | 55 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 55 nt
>oriT_pAK76
GCAGAAAACGGGCGTAGCACATTTTTCCGTATCCTGCCACTCTCAATAGAAAGGG
GCAGAAAACGGGCGTAGCACATTTTTCCGTATCCTGCCACTCTCAATAGAAAGGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2856 | GenBank | WP_198868924 |
| Name | t4cp2_JFX10_RS25000_pAK76 |
UniProt ID | _ |
| Length | 694 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 694 a.a. Molecular weight: 77279.15 Da Isoelectric Point: 9.7614
>WP_198868924.1 type IV secretory system conjugative DNA transfer family protein [Sinorhizobium meliloti]
MTKQLQACYLLFCVGIAFVVWMLGYGLGLQLFYKDGRILETTITSNPFAPIQQLWHYKTSPALQKVALGS
MVPALLVAGFVGYIGLKPTSSPLGDAAFQDIASLRRGKWFRKQGHIFGRIGRNILRTKDDRHHLIIGPTR
SGKGAGYVIPNALMHQGSMIVTDLKGEVFKATAGYRRQNGSQVFLFAPGSEKTNSYNPLDFIRPERGNRT
TDIQNIASILVPENTESENSVWQATAQQVLAGAISYITESHFYKDRRNLAEVNSFFNSGVDLQALMKYIK
EKEPYLSKFTVESFNSYIALSERAAASALMDIQKAMRPFKNERIIAATNVTDMDLRAMKRRPISIYLAPN
ITDITLLRPLLTLFVQQVMDILTLEHDPNSLPVYFLLDEFRQLKRMDEIMNKLPYVAGYNIKLAFIIQDL
KNLDEIYGETSRHSLLGNCGYQLVLGANDQATAEYASRALGKRTIRYQSESRTIELMGLPRRTKVEQIRE
RDLMMPQEVRQMPENKMILLIEGQRPIFGEKLRFFQTQPFKSAEAFSQANIPQVPEVDYLAPKPVPATTP
EYAKGGDPSVEMSSAQAKEEKPLTAAAAEAAPVKAEPAADEKAAAPVKRTVNKKALRPKPNATGGGEASA
SLNAMEARIKAIEEGLKPKAAQLKEVVETKAEKLGDKSPTRRRNIMDIFSATVPDPIEVGVPAE
MTKQLQACYLLFCVGIAFVVWMLGYGLGLQLFYKDGRILETTITSNPFAPIQQLWHYKTSPALQKVALGS
MVPALLVAGFVGYIGLKPTSSPLGDAAFQDIASLRRGKWFRKQGHIFGRIGRNILRTKDDRHHLIIGPTR
SGKGAGYVIPNALMHQGSMIVTDLKGEVFKATAGYRRQNGSQVFLFAPGSEKTNSYNPLDFIRPERGNRT
TDIQNIASILVPENTESENSVWQATAQQVLAGAISYITESHFYKDRRNLAEVNSFFNSGVDLQALMKYIK
EKEPYLSKFTVESFNSYIALSERAAASALMDIQKAMRPFKNERIIAATNVTDMDLRAMKRRPISIYLAPN
ITDITLLRPLLTLFVQQVMDILTLEHDPNSLPVYFLLDEFRQLKRMDEIMNKLPYVAGYNIKLAFIIQDL
KNLDEIYGETSRHSLLGNCGYQLVLGANDQATAEYASRALGKRTIRYQSESRTIELMGLPRRTKVEQIRE
RDLMMPQEVRQMPENKMILLIEGQRPIFGEKLRFFQTQPFKSAEAFSQANIPQVPEVDYLAPKPVPATTP
EYAKGGDPSVEMSSAQAKEEKPLTAAAAEAAPVKAEPAADEKAAAPVKRTVNKKALRPKPNATGGGEASA
SLNAMEARIKAIEEGLKPKAAQLKEVVETKAEKLGDKSPTRRRNIMDIFSATVPDPIEVGVPAE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60606..70516
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JFX10_RS24990 (JFX10_24980) | 56499..57152 | + | 654 | WP_198868922 | polymorphic toxin type 44 domain-containing protein | - |
| JFX10_RS24995 (JFX10_24985) | 57531..58361 | + | 831 | WP_198868923 | hypothetical protein | - |
| JFX10_RS25000 (JFX10_24990) | 58525..60609 | - | 2085 | WP_198868924 | type IV secretory system conjugative DNA transfer family protein | - |
| JFX10_RS25005 (JFX10_24995) | 60606..61634 | - | 1029 | WP_198868925 | P-type DNA transfer ATPase VirB11 | virB11 |
| JFX10_RS25010 (JFX10_25000) | 61615..62826 | - | 1212 | WP_198868926 | type IV secretion system protein VirB10 | virB10 |
| JFX10_RS25015 (JFX10_25005) | 62826..63635 | - | 810 | WP_198868927 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| JFX10_RS25020 (JFX10_25010) | 63632..64327 | - | 696 | WP_198868928 | type IV secretion system protein | virB8 |
| JFX10_RS25025 (JFX10_25015) | 64354..65382 | - | 1029 | WP_198868929 | type IV secretion system protein | virB6 |
| JFX10_RS25030 (JFX10_25020) | 65398..65625 | - | 228 | WP_142586835 | hypothetical protein | - |
| JFX10_RS25035 (JFX10_25025) | 65615..66316 | - | 702 | WP_127620009 | type IV secretion system protein | - |
| JFX10_RS25040 (JFX10_25030) | 66328..67416 | - | 1089 | WP_198868946 | lytic transglycosylase domain-containing protein | - |
| JFX10_RS25045 (JFX10_25035) | 67413..69872 | - | 2460 | WP_198868930 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| JFX10_RS25050 (JFX10_25040) | 69875..70174 | - | 300 | WP_142586832 | VirB3 family type IV secretion system protein | virB3 |
| JFX10_RS25055 (JFX10_25045) | 70178..70516 | - | 339 | WP_142586831 | TrbC/VirB2 family protein | virB2 |
| JFX10_RS25060 (JFX10_25050) | 70528..71436 | - | 909 | WP_198868947 | lytic transglycosylase domain-containing protein | - |
| JFX10_RS25065 (JFX10_25055) | 71659..72267 | + | 609 | WP_198868931 | hypothetical protein | - |
| JFX10_RS25070 (JFX10_25060) | 72264..72800 | + | 537 | WP_198868932 | thermonuclease family protein | - |
| JFX10_RS25075 (JFX10_25065) | 72797..73498 | + | 702 | WP_198868933 | thermonuclease family protein | - |
| JFX10_RS33570 | 73613..73825 | + | 213 | WP_234943692 | hypothetical protein | - |
| JFX10_RS25085 (JFX10_25075) | 73905..74498 | + | 594 | Protein_71 | hypothetical protein | - |
| JFX10_RS25090 (JFX10_25080) | 74521..74937 | + | 417 | WP_198868934 | hypothetical protein | - |
Host bacterium
| ID | 4474 | GenBank | NZ_CP066360 |
| Plasmid name | pAK76 | Incompatibility group | - |
| Plasmid size | 172809 bp | Coordinate of oriT [Strand] | 50512..50566 [-] |
| Host baterium | Sinorhizobium meliloti strain AK76 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA7 |