Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 104030
• Name: oriT_AUSMDU00027944|P01
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain AUSMDU00027944 isolate AUSMDU00027944
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_OU015341 (52115..52265 [+], 151 nt)
• oriT length: 151 nt
• IRs (inverted repeats): 93..98, 106..111 (TGGCCT..AGGCCA) 12..17, 24..29 (AACCCT..AGGGTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 151 nt

>oriT_AUSMDU00027944|P01
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_KKR00_RS25860_AUSMDU00027944|P01

• ID: 2986
• GenBank: WP_234942426
• Name: mobH_KKR00_RS25860_AUSMDU00027944|P01
• UniProt ID: _
• Length: 443 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 443 a.a.
• Molecular weight: 49931.89 Da
• Isoelectric Point: 6.2824

>WP_234942426.1 MobH family relaxase [Salmonella enterica]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEID
VTNGAIVIHTQRLKSDPGGNLLS

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (51-357)

Protein structure

No available structure.

T4CP

traD_KKR00_RS25880_AUSMDU00027944|P01

• ID: 2849
• GenBank: WP_000178857
• Name: traD_KKR00_RS25880_AUSMDU00027944|P01
• UniProt ID: _
• Length: 621 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 621 a.a.
• Molecular weight: 69225.71 Da
• Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (170-299)

• TraM recognition site of TraD and TraG (TraG-D_C) (471-598)

Protein structure

No available structure.

Host bacterium

• ID: 4469
• GenBank: NZ_OU015341
• Plasmid name: AUSMDU00027944|P01
• Incompatibility group: IncA/C2
• Plasmid size: 133718 bp
• Coordinate of oriT [Strand]: 52115..52265 [+]
• Host baterium: Salmonella enterica subsp. enterica serovar Typhimurium strain AUSMDU00027944 isolate AUSMDU00027944

Cargo genes

• Drug resistance gene: floR, tet(A), aph(6)-Id, aph(3'')-Ib, sul2, aac(3)-IId, qnrS1, blaCTX-M-55, catA2, blaTEM-1B
• Virulence gene: -
• Metal resistance gene: merD, merE
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -