Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103973
Name   oriT_16ZR26|unnamed in_silico
Organism   Klebsiella pneumoniae strain 16ZR26
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OP168824 (142997..143085 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_16ZR26|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2954 GenBank   WP_001405892
Name   nikB_PUP07_RS00860_16ZR26|unnamed insolico UniProt ID   A0A3V4WX06
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103883.32 Da        Isoelectric Point: 7.3373

>WP_001405892.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A3V4WX06


Auxiliary protein

ID   1226 GenBank   WP_001283947
Name   WP_001283947_16ZR26|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2813 GenBank   WP_001289276
Name   trbC_PUP07_RS00865_16ZR26|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 148463..188259

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PUP07_RS00865 (BENIHKCI_00175) 146179..148470 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
PUP07_RS00870 (BENIHKCI_00176) 148463..149533 - 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
PUP07_RS00875 (BENIHKCI_00177) 149552..150760 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
PUP07_RS00880 (BENIHKCI_00178) 151067..151846 - 780 WP_275450201 protein FinQ -
PUP07_RS00885 (BENIHKCI_00179) 152473..152625 + 153 WP_001303307 Hok/Gef family protein -
PUP07_RS00890 (BENIHKCI_00180) 152697..152948 - 252 WP_001291968 hypothetical protein -
PUP07_RS00895 (BENIHKCI_00181) 153872..154048 - 177 WP_001054900 hypothetical protein -
PUP07_RS00900 (BENIHKCI_00182) 154257..154466 - 210 WP_001140545 hemolysin expression modulator Hha -
PUP07_RS00905 (BENIHKCI_00183) 154564..155178 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
PUP07_RS00910 (BENIHKCI_00184) 155254..157422 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
PUP07_RS00915 (BENIHKCI_00185) 157519..158103 - 585 WP_001579774 IncI1-type conjugal transfer protein TraX -
PUP07_RS00920 (BENIHKCI_00186) 158132..159334 - 1203 WP_001189159 IncI1-type conjugal transfer protein TraW traW
PUP07_RS00925 (BENIHKCI_00187) 159301..159915 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
PUP07_RS00930 (BENIHKCI_00188) 159915..162959 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
PUP07_RS00935 (BENIHKCI_00189) 163049..163849 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
PUP07_RS00940 (BENIHKCI_00190) 163833..164021 - 189 WP_001277255 putative conjugal transfer protein TraS -
PUP07_RS00945 (BENIHKCI_00191) 164085..164489 - 405 WP_001579770 DUF6750 family protein traR
PUP07_RS00950 (BENIHKCI_00192) 164540..165067 - 528 WP_001055569 conjugal transfer protein TraQ traQ
PUP07_RS00955 (BENIHKCI_00193) 165067..165771 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
PUP07_RS00960 (BENIHKCI_00194) 165771..167060 - 1290 WP_001271997 conjugal transfer protein TraO traO
PUP07_RS00965 (BENIHKCI_00195) 167063..168046 - 984 WP_001579769 IncI1-type conjugal transfer protein TraN traN
PUP07_RS00970 (BENIHKCI_00196) 168057..168749 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
PUP07_RS00975 (BENIHKCI_00197) 168746..169093 - 348 WP_001055900 conjugal transfer protein traL
PUP07_RS00980 (BENIHKCI_00198) 169111..172878 - 3768 WP_001579768 LPD7 domain-containing protein -
PUP07_RS00985 (BENIHKCI_00199) 172968..173519 - 552 WP_000014584 phospholipase D family protein -
PUP07_RS00990 (BENIHKCI_00200) 173534..173824 - 291 WP_001372180 hypothetical protein traK
PUP07_RS00995 (BENIHKCI_00201) 173821..174969 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
PUP07_RS01000 (BENIHKCI_00202) 174966..175784 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
PUP07_RS01005 (BENIHKCI_00203) 175781..176239 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
PUP07_RS01010 (BENIHKCI_00204) 176634..177218 - 585 WP_001579766 histidine phosphatase family protein -
PUP07_RS01015 (BENIHKCI_00205) 177278..178480 - 1203 WP_000976353 conjugal transfer protein TraF -
PUP07_RS01020 (BENIHKCI_00206) 178566..179390 - 825 WP_001238929 conjugal transfer protein TraE traE
PUP07_RS01025 (BENIHKCI_00207) 179541..180695 - 1155 WP_001139958 site-specific integrase -
PUP07_RS01030 180694..181005 + 312 WP_000958120 hypothetical protein -
PUP07_RS01035 (BENIHKCI_00209) 182461..183753 - 1293 WP_001417545 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
PUP07_RS01040 (BENIHKCI_00210) 183753..184409 - 657 WP_001193553 prepilin peptidase -
PUP07_RS01045 (BENIHKCI_00211) 184394..184954 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
PUP07_RS01050 (BENIHKCI_00212) 184964..185578 - 615 WP_094819134 type 4 pilus major pilin -
PUP07_RS01055 (BENIHKCI_00213) 185596..186693 - 1098 WP_001208805 type II secretion system F family protein -
PUP07_RS01060 (BENIHKCI_00214) 186706..188259 - 1554 WP_001579763 ATPase, T2SS/T4P/T4SS family virB11
PUP07_RS01065 (BENIHKCI_00215) 188270..188722 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
PUP07_RS01070 (BENIHKCI_00216) 188709..190004 - 1296 WP_000752772 type 4b pilus protein PilO2 -
PUP07_RS01075 (BENIHKCI_00217) 189997..191679 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
PUP07_RS01080 (BENIHKCI_00218) 191693..192130 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
PUP07_RS01085 (BENIHKCI_00219) 192130..193197 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   4413 GenBank   NZ_OP168824
Plasmid name   16ZR26|unnamed Incompatibility group   IncHI1B
Plasmid size   278363 bp Coordinate of oriT [Strand]   142997..143085 [-]
Host baterium   Klebsiella pneumoniae strain 16ZR26

Cargo genes

Drug resistance gene   -
Virulence gene   rmpA, iutA, iucC, iucB, iucA
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pbrA, silE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2