Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103939
• Name: oriT_pTXW
• Organism: Lacticaseibacillus paracasei TXW
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_013952 (1671..1700 [+], 30 nt)
• oriT length: 30 nt
• IRs (inverted repeats): _
• Location of nic site: 19..20
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 30 nt

>oriT_pTXW
GAATGTAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_HS636_RS00020_pTXW

• ID: 2933
• GenBank: WP_013023906
• Name: mobV_HS636_RS00020_pTXW
• UniProt ID: D4NX04
• Length: 432 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 432 a.a.
• Molecular weight: 50346.64 Da
• Isoelectric Point: 9.1409

>WP_013023906.1 MobV family relaxase [Lacticaseibacillus paracasei]
MSQLAAHMQKFKIGNLGGLQRHDERTLQRHSNPDIDVSKSSNNFSVLPLERLDPKRSLHKQVQEIIADER
VSNRAVRKDAVVLTEWVISSDSTFFEGLSRDETKQFFTDAYQWFANHFGADHIPYATVHMDETTPHMHMG
VIPLTNGRLSAKAIFNREALRFIQADLPQKLNEKGWNIERGERGSKRKHLNVEQYKEVAESLKQGEPLFD
VSKLEHERVPIVNMFNFVKGKEATQNYILPPEDYQRLTERVQGFSDLGHAVKDVNELKTALSARESRIRK
REKELDKREDAISKNENLIETYDFWYDQAYEQSDKVTQLQGQIKTLKAENKQWQQNYYRLVDAVHDMAKT
IGTLITDYPAWEQKLNEFGQRVIRGTRAFAKNTLNKVQAYGAAEDIDTTVEISKSIEPDMYPEREKKRQQ
QRKRQRQQGLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (4-197)

Protein structure

• AlphaFold DB: D4NX04

Host bacterium

• ID: 4379
• GenBank: NC_013952
• Plasmid name: pTXW
• Incompatibility group: -
• Plasmid size: 3178 bp
• Coordinate of oriT [Strand]: 1671..1700 [+]
• Host baterium: Lacticaseibacillus paracasei TXW

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -