Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103931
Name   oriT_pCFSAN007426_01 in_silico
Organism   Salmonella enterica subsp. enterica serovar Agona strain CFSAN007426 isolate N19767
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP009412 (52121..52271 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pCFSAN007426_01
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2923 GenBank   WP_001326173
Name   mobH_JV40_RS00400_pCFSAN007426_01 insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   2772 GenBank   WP_000178857
Name   traD_JV40_RS00405_pCFSAN007426_01 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   2773 GenBank   WP_000637384
Name   traC_JV40_RS00510_pCFSAN007426_01 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50205..59295

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JV40_RS00390 (JV40_00370) 46236..46469 - 234 WP_001191890 hypothetical protein -
JV40_RS00395 (JV40_00375) 46451..47068 - 618 WP_001249395 hypothetical protein -
JV40_RS00400 (JV40_00380) 47236..50208 + 2973 WP_001326173 MobH family relaxase -
JV40_RS00405 (JV40_00385) 50205..52070 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
JV40_RS00410 (JV40_00390) 52081..52665 + 585 WP_000332868 hypothetical protein -
JV40_RS00415 (JV40_00395) 52622..53251 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
JV40_RS00420 (JV40_00400) 53261..53707 + 447 WP_000122507 hypothetical protein -
JV40_RS00425 (JV40_00405) 53717..54094 + 378 WP_000869297 hypothetical protein -
JV40_RS00430 (JV40_00410) 54094..54756 + 663 WP_001231464 hypothetical protein -
JV40_RS00435 54937..55080 + 144 WP_001275801 hypothetical protein -
JV40_RS00440 (JV40_00415) 55080..55457 + 378 WP_001326174 hypothetical protein -
JV40_RS00445 (JV40_00420) 55602..55883 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
JV40_RS00450 (JV40_00425) 55880..56506 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
JV40_RS00455 (JV40_00430) 56490..57407 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
JV40_RS00460 (JV40_00435) 57407..58720 + 1314 WP_024131605 TraB/VirB10 family protein traB
JV40_RS00465 (JV40_00440) 58717..59295 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
JV40_RS00470 (JV40_00445) 59299..59691 + 393 WP_000479535 TraA family conjugative transfer protein -
JV40_RS00475 (JV40_00450) 59976..61238 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
JV40_RS00480 (JV40_00455) 61562..62707 + 1146 WP_000976514 extended-spectrum class C beta-lactamase CMY-2 -
JV40_RS00485 (JV40_00460) 62801..63334 + 534 WP_001221666 lipocalin family protein -
JV40_RS00490 (JV40_00465) 63331..63648 - 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
JV40_RS00495 (JV40_00470) 63905..64270 + 366 Protein_91 LuxR family transcriptional regulator -

Host bacterium

ID   4371 GenBank   NZ_CP009412
Plasmid name   pCFSAN007426_01 Incompatibility group   IncA/C2
Plasmid size   103586 bp Coordinate of oriT [Strand]   52121..52271 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Agona strain CFSAN007426 isolate N19767

Cargo genes

Drug resistance gene   floR, tet(A), aph(6)-Id, aph(3'')-Ib, sul2, blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -