Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103914
Name   oriT_pV2200 in_silico
Organism   Staphylococcus aureus strain V2200
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP007658 (6802..6990 [-], 189 nt)
oriT length   189 nt
IRs (inverted repeats)      149..156, 161..168  (CTATCATT..AATGATAG)
 132..138, 142..148  (GTCTGGC..GCCAGAC)
 71..76, 88..93  (AAAAGC..GCTTTT)
 64..70, 77..83  (GTGTCAC..GTGACAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 189 nt

>oriT_pV2200
TGTCTTATTTTTGTGACAAATGCTGTATGTAGTGTCTCAAAACTGTGACAACCGCAATATATTGTGTCACAAAAGCGTGACACTACAGCTTTTTATGATATCACTTTAAAATTACTTAAAACCCTTAGAATGTCTGGCTTTGCCAGACCTATCATTTTTGAATGATAGCAAATTCTCCTTATGCTCTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2908 GenBank   WP_000122374
Name   mobV_EP20_RS14560_pV2200 insolico UniProt ID   A0A2R4A9A7
Length   413 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 413 a.a.        Molecular weight: 48526.00 Da        Isoelectric Point: 6.1679

>WP_000122374.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNYDLVNANKQNFNNLIDEKIEQNYTGK
RKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFFEYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITDDGRLSAKEVVGNKKALTAFQDRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERE
SQKTDHIKQKNDKLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKA
AQDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIEIALKLLKILL
KELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQEQQKNQKRDRGMHL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-198)


  Protein structure


Source ID Structure
AlphaFold DB A0A2R4A9A7


Host bacterium

ID   4354 GenBank   NZ_CP007658
Plasmid name   pV2200 Incompatibility group   -
Plasmid size   43396 bp Coordinate of oriT [Strand]   6802..6990 [-]
Host baterium   Staphylococcus aureus strain V2200

Cargo genes

Drug resistance gene   tet(K), msr(A), mph(C), blaZ
Virulence gene   sea
Metal resistance gene   merR, merT, merA, merB
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21