Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103913
• Name: oriT_pLAC2
• Organism: Ligilactobacillus acidipiscis strain ACA-DC 1533
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LT996085 (52957..52977 [+], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_pLAC2
AGTGGTGTGTAAGTGCACATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_GWA20_RS00250_pLAC2

• ID: 2907
• GenBank: WP_159372505
• Name: Relaxase_GWA20_RS00250_pLAC2
• UniProt ID: _
• Length: 514 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 514 a.a.
• Molecular weight: 58802.03 Da
• Isoelectric Point: 10.8233

>WP_159372505.1 relaxase/mobilization nuclease domain-containing protein [Ligilactobacillus acidipiscis]
MATITINSTGNMNSALDYAGNEKRAMKNNTREWLRQNGVKEQAINTINGRVVHKAGANGLTVFESHDPQL
ATSLREVKNHFKIERQLNHQTAKKNQAIRIKQNFPRNDLDPLNPEDWQKANDLGVELMERAYPDYKAAVY
THLDSQQHQLHNHIIMSRVNSMTGKKFRDANAGDSLTHFRDINDEISREQGWNVLQRNKLVSTRQVQRDL
AQKGIPSYITDVQEQALSVILNPKVKKLDDFQAEMGYQGFEVTQRGKSGVLSFHWTDQDNKKRQVRSKRM
GALFSAASLKKIFEIPPEQRAEVLQQQGVSLPGKAQNQAQLKEKPQQPQTASQPERKVTNNARQTTERPS
NNDTITQLMQNNHAKIGDNRAKTERLRTKTRRTRELRAGERPKYHELAREFTTGHRRLTANLEGQQKLAT
NQYQQSGHAITKGLGNRVTNIFNNGIKSLKQGAQGASKQLSHLGKGLMGVMGSIIQSAYQIQQQNRYIAK
RNAYYAQQQQRRNHRRQQEDEIER

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (70-293)

Protein structure

No available structure.

Host bacterium

• ID: 4353
• GenBank: NZ_LT996085
• Plasmid name: pLAC2
• Incompatibility group: -
• Plasmid size: 54237 bp
• Coordinate of oriT [Strand]: 52957..52977 [+]
• Host baterium: Ligilactobacillus acidipiscis strain ACA-DC 1533

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21