Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103894 |
| Name | oriT_pK43  |
| Organism | Salmonella enterica subsp. enterica serovar Typhi strain 343077_281186 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP029854 (28568..28916 [+], 349 nt) |
| oriT length | 349 nt |
| IRs (inverted repeats) | 134..140, 154..160 (AAAATAT..ATATTTT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 349 nt
>oriT_pK43
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA
ACTGCGATGCAGGATAGGGCAAACGCCGCAAAATGACGTCTCTGACGCCATTCCGCAACGTTTGCCCGATGACCTACAGTCGGTTCTTGTTGGAGAGTTTTATGAAATATTTGGTCAAAGAGTTTATTAACGAAAAATATACTAAGGCTGTTAATATTTTAAAGGATAACCTTAAAGAACACTATCATGTTTTTTATTGTGTGAGATTAAGTGAGATTCTTTTTCCTGCCAGTGAATATGGAACTGAACAGTTTTTTAGTGATTTTGAAAAGATAAACTCCATTTCATTACCTGTCTTAGTGTTTGATCTCAAAGAGGGTGTTCCTGTGATTGTCATTAGTCTTGATGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2896 | GenBank | WP_000539539 |
| Name | Relaxase_SM281186_RS24935_pK43 |
UniProt ID | A0A748H4F7 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44419.77 Da Isoelectric Point: 10.5368
>WP_000539539.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKAKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A748H4F7 |
T4CP
| ID | 2740 | GenBank | WP_000053830 |
| Name | t4cp2_SM281186_RS24870_pK43 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69403.32 Da Isoelectric Point: 7.1383
>WP_000053830.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFACDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFACDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10400..20422
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SM281186_RS24855 (SM281186_07037) | 6970..7542 | - | 573 | WP_001198960 | hypothetical protein | - |
| SM281186_RS25225 | 7546..7740 | - | 195 | Protein_8 | ATP-binding protein | - |
| SM281186_RS24860 (SM281186_07042) | 7853..8155 | - | 303 | WP_000820912 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| SM281186_RS24865 (SM281186_07043) | 8152..8565 | - | 414 | WP_000722606 | cag pathogenicity island Cag12 family protein | - |
| SM281186_RS24870 (SM281186_07121) | 8562..10397 | - | 1836 | WP_000053830 | type IV secretory system conjugative DNA transfer family protein | - |
| SM281186_RS24875 (SM281186_07031) | 10400..11431 | - | 1032 | WP_046597967 | P-type DNA transfer ATPase VirB11 | virB11 |
| SM281186_RS24880 (SM281186_07039) | 11433..12638 | - | 1206 | WP_000139139 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| SM281186_RS24885 (SM281186_07047) | 12635..13564 | - | 930 | WP_000783380 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| SM281186_RS24890 (SM281186_07050) | 13569..14282 | - | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| SM281186_RS24895 (SM281186_07116) | 14528..15658 | - | 1131 | WP_121509152 | type IV secretion system protein | virB6 |
| SM281186_RS24900 (SM281186_07040) | 15670..15954 | - | 285 | WP_025840300 | EexN family lipoprotein | - |
| SM281186_RS24905 (SM281186_07041) | 15969..16718 | - | 750 | WP_046597965 | type IV secretion system protein | - |
| SM281186_RS24910 (SM281186_07044) | 16729..19482 | - | 2754 | WP_046597964 | VirB3 family type IV secretion system protein | virb4 |
| SM281186_RS24915 (SM281186_07048) | 19501..19797 | - | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| SM281186_RS24920 (SM281186_07056) | 19775..20422 | - | 648 | WP_000953526 | lytic transglycosylase domain-containing protein | virB1 |
| SM281186_RS24930 (SM281186_07055) | 20597..21115 | - | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| SM281186_RS24935 (SM281186_07057) | 21468..22634 | - | 1167 | WP_000539539 | MobP1 family relaxase | - |
| SM281186_RS24940 (SM281186_07119) | 22637..23182 | - | 546 | WP_046597963 | DNA distortion polypeptide 1 | - |
| SM281186_RS24945 (SM281186_07045) | 23508..23780 | - | 273 | WP_000160399 | hypothetical protein | - |
| SM281186_RS25230 | 23793..23942 | - | 150 | WP_000003880 | hypothetical protein | - |
| SM281186_RS24950 | 24012..24191 | - | 180 | WP_000439078 | hypothetical protein | - |
| SM281186_RS24955 (SM281186_07054) | 24234..24563 | - | 330 | WP_046597962 | hypothetical protein | - |
| SM281186_RS24960 (SM281186_07124) | 24656..24871 | - | 216 | WP_001180117 | hypothetical protein | - |
| SM281186_RS24965 (SM281186_07061) | 24861..25106 | - | 246 | WP_000356542 | hypothetical protein | - |
Host bacterium
| ID | 4334 | GenBank | NZ_CP029854 |
| Plasmid name | pK43 | Incompatibility group | IncX3 |
| Plasmid size | 43427 bp | Coordinate of oriT [Strand] | 28568..28916 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhi strain 343077_281186 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |