Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103868 |
| Name | oriT_pFORC75_2  |
| Organism | Salmonella enterica subsp. enterica serovar Enteritidis strain FORC_075 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP023476 (29386..29992 [+], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..202, 204..210 (TCAATTC..GAATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_pFORC75_2
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2872 | GenBank | WP_000539540 |
| Name | Relaxase_FORC75_RS24265_pFORC75_2 |
UniProt ID | A0A744DX50 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44505.86 Da Isoelectric Point: 10.5067
>WP_000539540.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A744DX50 |
T4CP
| ID | 2714 | GenBank | WP_000053820 |
| Name | t4cp2_FORC75_RS24200_pFORC75_2 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69389.23 Da Isoelectric Point: 7.1491
>WP_000053820.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 16885..26846
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FORC75_RS24185 (FORC75_p019) | 13452..14027 | - | 576 | WP_080165183 | DNA topoisomerase III | - |
| FORC75_RS24455 | 14031..14225 | - | 195 | Protein_19 | ATP-binding protein | - |
| FORC75_RS24190 (FORC75_p020) | 14338..14640 | - | 303 | WP_000717627 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| FORC75_RS24195 (FORC75_p021) | 14637..15050 | - | 414 | WP_024136126 | cag pathogenicity island Cag12 family protein | - |
| FORC75_RS24200 (FORC75_p022) | 15047..16882 | - | 1836 | WP_000053820 | type IV secretory system conjugative DNA transfer family protein | - |
| FORC75_RS24205 (FORC75_p023) | 16885..17916 | - | 1032 | WP_001058468 | P-type DNA transfer ATPase VirB11 | virB11 |
| FORC75_RS24210 (FORC75_p024) | 17918..19123 | - | 1206 | WP_031622284 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| FORC75_RS24215 (FORC75_p025) | 19120..20049 | - | 930 | WP_000783385 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| FORC75_RS24220 (FORC75_p026) | 20054..20767 | - | 714 | WP_000394612 | type IV secretion system protein | virB8 |
| FORC75_RS24225 (FORC75_p027) | 21013..22146 | - | 1134 | WP_000513458 | type IV secretion system protein | virB6 |
| FORC75_RS24230 (FORC75_p028) | 22156..22383 | - | 228 | WP_000835348 | EexN family lipoprotein | - |
| FORC75_RS24235 (FORC75_p029) | 22384..23142 | - | 759 | WP_000744210 | type IV secretion system protein | - |
| FORC75_RS24240 (FORC75_p030) | 23153..25906 | - | 2754 | WP_001328100 | VirB3 family type IV secretion system protein | virb4 |
| FORC75_RS24245 (FORC75_p031) | 25925..26221 | - | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| FORC75_RS24250 (FORC75_p032) | 26199..26846 | - | 648 | WP_000953529 | lytic transglycosylase domain-containing protein | virB1 |
| FORC75_RS24255 (FORC75_p033) | 26879..27091 | - | 213 | WP_000125172 | hypothetical protein | - |
| FORC75_RS24260 (FORC75_p034) | 27021..27539 | - | 519 | WP_001712455 | transcription termination/antitermination NusG family protein | - |
| FORC75_RS24265 (FORC75_p035) | 27892..29058 | - | 1167 | WP_000539540 | MobP1 family relaxase | - |
| FORC75_RS24270 (FORC75_p036) | 29061..29606 | - | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| FORC75_RS24280 (FORC75_p038) | 29932..30204 | - | 273 | WP_000160399 | hypothetical protein | - |
| FORC75_RS24285 | 30217..30414 | - | 198 | WP_077688582 | hypothetical protein | - |
| FORC75_RS24290 (FORC75_p039) | 30436..30615 | - | 180 | WP_021526659 | hypothetical protein | - |
| FORC75_RS24295 (FORC75_p040) | 30658..30987 | - | 330 | WP_000866654 | hypothetical protein | - |
| FORC75_RS24300 (FORC75_p041) | 31080..31295 | - | 216 | WP_001180117 | hypothetical protein | - |
| FORC75_RS24305 (FORC75_p042) | 31285..31530 | - | 246 | WP_000356542 | hypothetical protein | - |
Host bacterium
| ID | 4308 | GenBank | NZ_CP023476 |
| Plasmid name | pFORC75_2 | Incompatibility group | IncX1 |
| Plasmid size | 34661 bp | Coordinate of oriT [Strand] | 29386..29992 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Enteritidis strain FORC_075 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |