Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103846
• Name: oriT_KH46|psymB
• Organism: Sinorhizobium meliloti strain KH46
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP021823 (414597..414625 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_KH46|psymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_CDO24_RS20310_KH46|psymB

• ID: 2856
• GenBank: WP_088199714
• Name: traA_CDO24_RS20310_KH46|psymB
• UniProt ID: _
• Length: 1539 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1539 a.a.
• Molecular weight: 169930.69 Da
• Isoelectric Point: 9.6593

>WP_088199714.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLKAAIDG
RSVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTEEGFGPKKVPVLGEDGEPLRVVTPDRPNGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGVAMYFAPADLARRQEMADRLLAEPGLLLKQ
LGNERSTFDERDIARALHRYVDDPVDFANIRARLMASDELVLLKPQQVDAETGKAKQPAVFTTREMLRLE
YAMARSAEVLSRRKGFGVSNARAAAAVRSIETADTEKPFRLDPEQVDAVRHVTRDNAIAAVVGLAGAGKS
TLLAAARVAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGREQLQRGDVLVIDEAGMVS
SQQMARVLKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAITERIGFAELAGVRRQRDAWARDASRLFARGK
VEEGLDAYAQQGRIVETETRAEIVGRIVADWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
EALRQVMIGEGALTGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISEDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEAKPEWGRKPRVDHAAGVTGELVKEGMAKFRPNDEDADESPYADIRTDDGTVQRLWGVSLPKA
LKDAGVAEGDTITLRKDGVERVKVQVPIVDAQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFKQLVERLSRSGAKTTTLDFEGEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVA
KLWERASVALGFAIERERRVSYNEERTEALSTGIPSDGKYLIPPTTTFSRSVAEDARLAQLSSQRWKERE
AILHPVLAKIYRDPDGALSALNALASDAAIEPRKLADDLGKAPDRLGRLRGSELVVDGRAARDERTAATV
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALSKTAMARLVEIEAVREQGGDDAYRTAFAF
AVEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERREKLTRLFAVIRRFAEEQH
LAERQDRSKIVAGASVELGKETFAVLPMLAPVTEFKTTVAEEARERALAAPHYAHHRAALVETATRVWRD
PADAIGKIEDLIVKGFAGERIAAAVTNDPAAYGALRGSDRIMDKLLAAGRERKDALQAVPEAASRIRSLG
ASYASALDAETRSITEERRRMAVAIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPRIAREFAKVSR
ALDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLQVLQQTVRVQSSQKIVSERRQRAINQSRGIRM

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-578)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1125)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1309-1400)

Protein structure

No available structure.

T4CP

tcpA_CDO24_RS35100_KH46|psymB

• ID: 2687
• GenBank: WP_094179299
• Name: tcpA_CDO24_RS35100_KH46|psymB
• UniProt ID: _
• Length: 946 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 946 a.a.
• Molecular weight: 101607.37 Da
• Isoelectric Point: 4.5608

>WP_094179299.1 DNA translocase FtsK [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEES
RIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQAPEASVTKPAI
TESVAVAEEEPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMPL
EVAAVPPTVTEPVKEAARIPAPIAAAPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEPV
TAAPQDIAAEPAAAEAAALPAPQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPPI
GAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEF
EPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLAL
CLGKAIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIP
HLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTGE
PLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKA
NFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRPE
YLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVE
RMEKDGLVGPANHVGKREIIYGNRDNAPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK alpha domain (FtsK_alpha) (434-534)

• Ftsk gamma domain (FtsK_gamma) (871-930)

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (543-761)

Protein structure

No available structure.

Host bacterium

• ID: 4286
• GenBank: NZ_CP021823
• Plasmid name: KH46|psymB
• Incompatibility group: -
• Plasmid size: 1683883 bp
• Coordinate of oriT [Strand]: 414597..414625 [+]
• Host baterium: Sinorhizobium meliloti strain KH46

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7, AcrVIB