Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103834 |
| Name | oriT_CFNEI 73|C  |
| Organism | Sinorhizobium americanum strain CFNEI 73 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP013110 (1741743..1741771 [-], 29 nt) |
| oriT length | 29 nt |
| IRs (inverted repeats) | 14..19, 24..29 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 29 nt
>oriT_CFNEI 73|C
AGGGCGCAATATACGTCGCTGGCGCGACG
AGGGCGCAATATACGTCGCTGGCGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2845 | GenBank | WP_064252672 |
| Name | traA_SAMCFNEI73_RS29515_CFNEI 73|C |
UniProt ID | A0A1L3LZ33 |
| Length | 1536 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1536 a.a. Molecular weight: 169919.36 Da Isoelectric Point: 9.0148
>WP_064252672.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium americanum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGSRELAHEELALPDQVPVWLKRATAG
QSVAKASEALWNALDAFETRADAQLARELIIALPEELTRAENIALVREFVRDSFTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTEEGFGPKKVPVLGEDGEPLRVATPDRPNGNIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIEMYFAPTDLARRQEMADRLLAEPELLLKQ
LGNERSTFDETHIAKALHRYVDDPVDFANIRARLMASNDLVTLRPQQLDAETGKASEPAVFTTREILRIE
YDMARSAELLSQRKGFGVSQRQIAAAVQSVETADPTKPFKLDAEQVDAVSHVTGNNAIAAVVGLAGAGKS
TLLAAARVAWESEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWASGRDTLQRGDVLAIDEAGMVS
SQQMARVLKAVEAAGAKAVLVGDAMQLQPIQAGAAFRAISERVGYAELAGVRRQRDEWAREASRLFARGE
VEKGLDAYASHGRLVESQTREEIIGRIVSDWADARKEAIDKSVWEGRLRDDELLVLAHTNDDVRKLNEAL
RKVMTDEGALSNSRTFQTARGAREFAAGDRIIFLENARFLEPRARRLAPQYVKNGMLGTVVSTGDKRGDP
LLSVRLDNGRDVVISEGSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADLYAA
KEDFGAKPEWGRKPRVDHAAGVTGELVEEGMAKFRPNDEDADESPYADIRTDDGTVHRVWGVSLPKALED
AGVSEGDTVTLRKDGVEKVKVEVPVVDPETGQKRFEEREVDRNVWTGKQLETAAARQERIEREGHRPELF
SELVDRLGRSGAKTTTLDFESAAGYRAHAQDFARRRGIDTLAEVAAGMEEGVARRLAWIAEKREQVAKLW
ERASVALGFAIERERRVSYSEDRAESLSAGIPAVGRYLLPPTTEFARSVDEDALYAQLSSPRWKEREDIL
RPVLAKIYRDPDASLGNLNALASDAAIEPRTLAGDVAKAPDLLGRLRGSDNIVEGRAARDERNAAMVAFK
ELLPLVRAHATEFRRNADRFREREQTRRAYISLSIPAISKQAMARLVEIEAVRKQGGDNAWKAAFAIAAE
DRSLVQEVKAVSDALTARFGWSAFTAKADAIAERNMAERMPEDLTNERRGKLTRLFEVIRRFSEEQHLAE
RKDRSNIVAGASVEAGKETIAVLPMLAAVTEFRIPVDDEARPRALAAQHYRHNRAALAETATRIWRDPAG
AVDRIEDLIVKGFAVERIAEAVANDPAAYGALRGSDRLMDRMFAAGRERKDALQAVPEAAVYLRMLGAAY
VGAFDAERQAVTKERRRMAVAIPGLSPAAEEALRRLVPETKKKGSKLDVAAAALKPAIRQEFAAVSRALD
ERFGRNAILRGEKDVIKRVSPLQRRAFEAMHERLKVLQQTVRMQSSEQILSERRQRTINRARGVTW
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGSRELAHEELALPDQVPVWLKRATAG
QSVAKASEALWNALDAFETRADAQLARELIIALPEELTRAENIALVREFVRDSFTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTEEGFGPKKVPVLGEDGEPLRVATPDRPNGNIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIEMYFAPTDLARRQEMADRLLAEPELLLKQ
LGNERSTFDETHIAKALHRYVDDPVDFANIRARLMASNDLVTLRPQQLDAETGKASEPAVFTTREILRIE
YDMARSAELLSQRKGFGVSQRQIAAAVQSVETADPTKPFKLDAEQVDAVSHVTGNNAIAAVVGLAGAGKS
TLLAAARVAWESEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWASGRDTLQRGDVLAIDEAGMVS
SQQMARVLKAVEAAGAKAVLVGDAMQLQPIQAGAAFRAISERVGYAELAGVRRQRDEWAREASRLFARGE
VEKGLDAYASHGRLVESQTREEIIGRIVSDWADARKEAIDKSVWEGRLRDDELLVLAHTNDDVRKLNEAL
RKVMTDEGALSNSRTFQTARGAREFAAGDRIIFLENARFLEPRARRLAPQYVKNGMLGTVVSTGDKRGDP
LLSVRLDNGRDVVISEGSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADLYAA
KEDFGAKPEWGRKPRVDHAAGVTGELVEEGMAKFRPNDEDADESPYADIRTDDGTVHRVWGVSLPKALED
AGVSEGDTVTLRKDGVEKVKVEVPVVDPETGQKRFEEREVDRNVWTGKQLETAAARQERIEREGHRPELF
SELVDRLGRSGAKTTTLDFESAAGYRAHAQDFARRRGIDTLAEVAAGMEEGVARRLAWIAEKREQVAKLW
ERASVALGFAIERERRVSYSEDRAESLSAGIPAVGRYLLPPTTEFARSVDEDALYAQLSSPRWKEREDIL
RPVLAKIYRDPDASLGNLNALASDAAIEPRTLAGDVAKAPDLLGRLRGSDNIVEGRAARDERNAAMVAFK
ELLPLVRAHATEFRRNADRFREREQTRRAYISLSIPAISKQAMARLVEIEAVRKQGGDNAWKAAFAIAAE
DRSLVQEVKAVSDALTARFGWSAFTAKADAIAERNMAERMPEDLTNERRGKLTRLFEVIRRFSEEQHLAE
RKDRSNIVAGASVEAGKETIAVLPMLAAVTEFRIPVDDEARPRALAAQHYRHNRAALAETATRIWRDPAG
AVDRIEDLIVKGFAVERIAEAVANDPAAYGALRGSDRLMDRMFAAGRERKDALQAVPEAAVYLRMLGAAY
VGAFDAERQAVTKERRRMAVAIPGLSPAAEEALRRLVPETKKKGSKLDVAAAALKPAIRQEFAAVSRALD
ERFGRNAILRGEKDVIKRVSPLQRRAFEAMHERLKVLQQTVRMQSSEQILSERRQRTINRARGVTW
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2672 | GenBank | WP_064253455 |
| Name | tcpA_SAMCFNEI73_RS24015_CFNEI 73|C |
UniProt ID | _ |
| Length | 931 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 931 a.a. Molecular weight: 100214.15 Da Isoelectric Point: 4.6169
>WP_064253455.1 DNA translocase FtsK [Sinorhizobium americanum]
MRIPRTNFSTAALHDADEADDFEHPYQGSPDGSHAQTTRASAQDHLFEAPDAPRRGAGHEADEMGYAGRA
ARLYGHGSAYAPAQVTASTRDPLPTLAEITGQTDGPSWESHFFLSPNVRFTRTPEREFMKRRASAADESG
GGVEETGAEVAEPDVVAETAPADAAPATVEPEAPAHSPSELLRVLMQQLPSWRPAQGRDTALAESALDAE
AVVKEEVSVAEEPEGTPARAVPERDAAELNPDPTSDSAAIVVSNEGNAHLSYLSDHAFFEFMPLETVAVS
QVAVEPVKEPAKAPVQIATAPKPVAVPPVEIRPQPPTAITSMFRVVECRPAATAVVAPVPAPVQIVNRSA
EEAVTATPAPAPASVEPAESVKAEEDTPDLDVPVTKAAITMPAVVQRSTSALPPGGRGERQQAGDTYEFP
AKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEFEPAPGVKSSRVIGLA
DDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESNDFQRTGCKLALCLGKTIGGEPVIAEL
AKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVM
ALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGEPILATVQTGFEKGTGEPLFEQQEMDLTPMPY
IVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKANFPTRISFQVTSKID
SRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDLEVEHVVAHLKTQGRPEYLETVTADEEEEEPE
EDQGAVFDKSAIAAEDGNELYEQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVERMEKDGLVGPANHVG
KREIIHGNRDHAAKADDGEME
MRIPRTNFSTAALHDADEADDFEHPYQGSPDGSHAQTTRASAQDHLFEAPDAPRRGAGHEADEMGYAGRA
ARLYGHGSAYAPAQVTASTRDPLPTLAEITGQTDGPSWESHFFLSPNVRFTRTPEREFMKRRASAADESG
GGVEETGAEVAEPDVVAETAPADAAPATVEPEAPAHSPSELLRVLMQQLPSWRPAQGRDTALAESALDAE
AVVKEEVSVAEEPEGTPARAVPERDAAELNPDPTSDSAAIVVSNEGNAHLSYLSDHAFFEFMPLETVAVS
QVAVEPVKEPAKAPVQIATAPKPVAVPPVEIRPQPPTAITSMFRVVECRPAATAVVAPVPAPVQIVNRSA
EEAVTATPAPAPASVEPAESVKAEEDTPDLDVPVTKAAITMPAVVQRSTSALPPGGRGERQQAGDTYEFP
AKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEFEPAPGVKSSRVIGLA
DDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESNDFQRTGCKLALCLGKTIGGEPVIAEL
AKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVM
ALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGEPILATVQTGFEKGTGEPLFEQQEMDLTPMPY
IVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKANFPTRISFQVTSKID
SRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDLEVEHVVAHLKTQGRPEYLETVTADEEEEEPE
EDQGAVFDKSAIAAEDGNELYEQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVERMEKDGLVGPANHVG
KREIIHGNRDHAAKADDGEME
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 2673 | GenBank | WP_064252674 |
| Name | traG_SAMCFNEI73_RS29530_CFNEI 73|C |
UniProt ID | _ |
| Length | 643 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 643 a.a. Molecular weight: 70839.57 Da Isoelectric Point: 9.5987
>WP_064252674.1 Ti-type conjugative transfer system protein TraG [Sinorhizobium americanum]
MTPKAKPDPNLPLVLVPVAITAFAVYVVGWRWSEWAAGLSEKSAYWFLRASPVPTLSLGPLSGLLTVHML
PLHRRRPIAMASLVCFLTVAGYYALREYGRLAPAVESGATRWDQALTYLDMVAVISAVLGFLAVAVAARI
STVVPDLVKRGKRGTFGDADWLPMSAAGKLFPPDGEIVVGERYRVDKELVHALPFDPNDTTTWGRGGKAP
LLTYRQNFDSTHMLFFAGSGGYKTTSNVIPTALRYSGPLICLDPSTEVAPMVVEHRTRVLGREVIVLDPT
NPIIGFNALDGIETSARQEEDIVGIAHMLLSDSARMESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRT
LRSLRQIVSEPEPSVLAMLRDIQENSGSTFIRETRGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GSTFKSSDIVTARKDVFLNIPASILRSYPGIGRIVIGSLINAMVRADGGFKRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLVRHFGKDGATSWIEGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNNGSRKSETVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKDMNGAAK
ANRFVKARYEAAD
MTPKAKPDPNLPLVLVPVAITAFAVYVVGWRWSEWAAGLSEKSAYWFLRASPVPTLSLGPLSGLLTVHML
PLHRRRPIAMASLVCFLTVAGYYALREYGRLAPAVESGATRWDQALTYLDMVAVISAVLGFLAVAVAARI
STVVPDLVKRGKRGTFGDADWLPMSAAGKLFPPDGEIVVGERYRVDKELVHALPFDPNDTTTWGRGGKAP
LLTYRQNFDSTHMLFFAGSGGYKTTSNVIPTALRYSGPLICLDPSTEVAPMVVEHRTRVLGREVIVLDPT
NPIIGFNALDGIETSARQEEDIVGIAHMLLSDSARMESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRT
LRSLRQIVSEPEPSVLAMLRDIQENSGSTFIRETRGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GSTFKSSDIVTARKDVFLNIPASILRSYPGIGRIVIGSLINAMVRADGGFKRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLVRHFGKDGATSWIEGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNNGSRKSETVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKDMNGAAK
ANRFVKARYEAAD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1753202..1762757
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SAMCFNEI73_RS29555 (SAMCFNEI73_pC1637) | 1748226..1749665 | - | 1440 | WP_072642803 | IS1182 family transposase | - |
| SAMCFNEI73_RS29560 (SAMCFNEI73_pC1639) | 1750047..1751237 | + | 1191 | WP_064252749 | DUF1173 domain-containing protein | - |
| SAMCFNEI73_RS29565 (SAMCFNEI73_pC1640) | 1751401..1752591 | - | 1191 | WP_037382849 | hypothetical protein | - |
| SAMCFNEI73_RS29570 (SAMCFNEI73_pC1642) | 1753202..1754191 | - | 990 | WP_064252748 | P-type DNA transfer ATPase VirB11 | virB11 |
| SAMCFNEI73_RS29575 (SAMCFNEI73_pC1643) | 1754204..1755379 | - | 1176 | WP_064252747 | type IV secretion system protein VirB10 | virB10 |
| SAMCFNEI73_RS29580 (SAMCFNEI73_pC1644) | 1755388..1756236 | - | 849 | WP_153045197 | P-type conjugative transfer protein VirB9 | virB9 |
| SAMCFNEI73_RS29585 (SAMCFNEI73_pC1645) | 1756248..1756919 | - | 672 | WP_064252745 | virB8 family protein | virB8 |
| SAMCFNEI73_RS29595 (SAMCFNEI73_pC1646) | 1757234..1758163 | - | 930 | WP_064252744 | type IV secretion system protein | virB6 |
| SAMCFNEI73_RS29600 (SAMCFNEI73_pC1647) | 1758169..1758402 | - | 234 | WP_037382810 | EexN family lipoprotein | - |
| SAMCFNEI73_RS29605 (SAMCFNEI73_pC1648) | 1758399..1759094 | - | 696 | WP_064252743 | P-type DNA transfer protein VirB5 | virB5 |
| SAMCFNEI73_RS29610 (SAMCFNEI73_pC1649) | 1759094..1761460 | - | 2367 | WP_064252742 | VirB4 family type IV secretion system protein | virb4 |
| SAMCFNEI73_RS29615 (SAMCFNEI73_pC1650) | 1761453..1761791 | - | 339 | WP_064252741 | type IV secretion system protein VirB3 | virB3 |
| SAMCFNEI73_RS29620 (SAMCFNEI73_pC1651) | 1761796..1762098 | - | 303 | WP_064252740 | TrbC/VirB2 family protein | virB2 |
| SAMCFNEI73_RS29625 (SAMCFNEI73_pC1652) | 1762095..1762757 | - | 663 | WP_064252739 | lytic transglycosylase domain-containing protein | virB1 |
| SAMCFNEI73_RS33445 | 1762757..1763263 | - | 507 | WP_072642939 | hypothetical protein | - |
| SAMCFNEI73_RS29635 (SAMCFNEI73_pC1653) | 1763360..1763764 | + | 405 | WP_064252738 | MarR family transcriptional regulator | - |
| SAMCFNEI73_RS29640 (SAMCFNEI73_pC1654) | 1764121..1764360 | + | 240 | WP_064252737 | hypothetical protein | - |
| SAMCFNEI73_RS29645 | 1764774..1765004 | - | 231 | WP_064252736 | hypothetical protein | - |
| SAMCFNEI73_RS29650 (SAMCFNEI73_pC1656) | 1765424..1766469 | + | 1046 | Protein_1605 | IS110 family transposase | - |
| SAMCFNEI73_RS29655 | 1766779..1767372 | + | 594 | WP_153045196 | SMI1/KNR4 family protein | - |
Host bacterium
| ID | 4274 | GenBank | NZ_CP013110 |
| Plasmid name | CFNEI 73|C | Incompatibility group | - |
| Plasmid size | 2177502 bp | Coordinate of oriT [Strand] | 1741743..1741771 [-] |
| Host baterium | Sinorhizobium americanum strain CFNEI 73 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | actP, arsC, aioX/aoxX, aioS/aoxS, aioR/aoxR |
| Degradation gene | - |
| Symbiosis gene | fixA, fixB |
| Anti-CRISPR | AcrIIA7 |