Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103833 |
| Name | oriT_CFNEI 73|A  |
| Organism | Sinorhizobium americanum strain CFNEI 73 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP013108 (44950..45006 [-], 57 nt) |
| oriT length | 57 nt |
| IRs (inverted repeats) | 4..9, 23..28 (GGAAAA..TTTTCC) |
| Location of nic site | 36..37 |
| Conserved sequence flanking the nic site |
TCCTGCCCCT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 57 nt
>oriT_CFNEI 73|A
GCAGGAAAAGGGCGTAGCACATTTTTCCGTATCCTGCCCCTCCAAATTGTAGGGGGA
GCAGGAAAAGGGCGTAGCACATTTTTCCGTATCCTGCCCCTCCAAATTGTAGGGGGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2671 | GenBank | WP_064255156 |
| Name | t4cp2_SAMCFNEI73_RS18275_CFNEI 73|A |
UniProt ID | _ |
| Length | 699 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 699 a.a. Molecular weight: 77570.64 Da Isoelectric Point: 9.8605
>WP_064255156.1 type IV secretory system conjugative DNA transfer family protein [Sinorhizobium americanum]
MTKQLQAFYLLFCVGIAFVVWMLGYGLGLQLFYKDGRILETTITSNPFAPIQQFWHYKTSPALQKVALGS
VVPALLVAGLVAYIGLKPTSSPLGDAAFRDMASLRRGKWFRKQGHIFGRIGRNILRTKDDRHHLIIGPTR
SGKGAGYVIPNALMHEGSMIVTDLKGEVFKASAGYRRQNGSQVFLFAPGSEKTNSYNALDFIRPERGNRT
TDIQNIASILVPENTESENSVWQATAQQVLAGVISYITESHFYKDRRNLAEVNSFFNSGVDLQALMKYIK
EKEPDLSKFTVESFNSYIALSERAAASALMDIQKAMRPFKNERIIAATNVTDMDLRAMKRRPISIYLAPN
ITDITLLRPLLTLFVQQVMDILTLEHDPNSLPVYFLLDEFRQLKRMDEIMTKLPYVAGYNIKLAFIIQDL
KNLDEIYGETSRHSLLGNCGYQLVLGANDQATAEYASRALGKRTIRYQSESRTIELMGLPRRTKVEQIRE
RDLMMPQEVRQMPENKMILLIEGQRPIFGEKLRFFQTQPFKSAEVFSQANIPQVPEVDYLSPKPVPATTP
EYAKGGDPSVEIAAPAPVTEEKPLTVAAAEAAPVKAEPAAAAKAAAPAKRTVNKKALRPKPKATAAKTGG
AEASASLDAMEARIKAIEEGLKPKAAQLKEVVETKAEKLGDKSPTKRRNIMDIFSATVPDPVEVGVAAE
MTKQLQAFYLLFCVGIAFVVWMLGYGLGLQLFYKDGRILETTITSNPFAPIQQFWHYKTSPALQKVALGS
VVPALLVAGLVAYIGLKPTSSPLGDAAFRDMASLRRGKWFRKQGHIFGRIGRNILRTKDDRHHLIIGPTR
SGKGAGYVIPNALMHEGSMIVTDLKGEVFKASAGYRRQNGSQVFLFAPGSEKTNSYNALDFIRPERGNRT
TDIQNIASILVPENTESENSVWQATAQQVLAGVISYITESHFYKDRRNLAEVNSFFNSGVDLQALMKYIK
EKEPDLSKFTVESFNSYIALSERAAASALMDIQKAMRPFKNERIIAATNVTDMDLRAMKRRPISIYLAPN
ITDITLLRPLLTLFVQQVMDILTLEHDPNSLPVYFLLDEFRQLKRMDEIMTKLPYVAGYNIKLAFIIQDL
KNLDEIYGETSRHSLLGNCGYQLVLGANDQATAEYASRALGKRTIRYQSESRTIELMGLPRRTKVEQIRE
RDLMMPQEVRQMPENKMILLIEGQRPIFGEKLRFFQTQPFKSAEVFSQANIPQVPEVDYLSPKPVPATTP
EYAKGGDPSVEIAAPAPVTEEKPLTVAAAEAAPVKAEPAAAAKAAAPAKRTVNKKALRPKPKATAAKTGG
AEASASLDAMEARIKAIEEGLKPKAAQLKEVVETKAEKLGDKSPTKRRNIMDIFSATVPDPVEVGVAAE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 90802..100726
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SAMCFNEI73_RS18260 (SAMCFNEI73_pA0091) | 86110..87339 | + | 1230 | WP_064255154 | aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme | - |
| SAMCFNEI73_RS18265 (SAMCFNEI73_pA0092) | 87384..88310 | + | 927 | WP_064255155 | dihydrodipicolinate synthase family protein | - |
| SAMCFNEI73_RS33535 (SAMCFNEI73_pA0093) | 88517..88609 | - | 93 | Protein_88 | helix-turn-helix domain-containing protein | - |
| SAMCFNEI73_RS18275 (SAMCFNEI73_pA0094) | 88706..90805 | - | 2100 | WP_064255156 | type IV secretory system conjugative DNA transfer family protein | - |
| SAMCFNEI73_RS18280 (SAMCFNEI73_pA0095) | 90802..91830 | - | 1029 | WP_064255157 | P-type DNA transfer ATPase VirB11 | virB11 |
| SAMCFNEI73_RS18285 (SAMCFNEI73_pA0096) | 91811..93028 | - | 1218 | WP_037382037 | type IV secretion system protein VirB10 | virB10 |
| SAMCFNEI73_RS18290 (SAMCFNEI73_pA0097) | 93028..93837 | - | 810 | WP_064255158 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| SAMCFNEI73_RS18295 (SAMCFNEI73_pA0098) | 93834..94529 | - | 696 | WP_037382034 | type IV secretion system protein | virB8 |
| SAMCFNEI73_RS18300 (SAMCFNEI73_pA0099) | 94555..95586 | - | 1032 | WP_037382032 | type IV secretion system protein | virB6 |
| SAMCFNEI73_RS33200 (SAMCFNEI73_pA0100) | 95602..95799 | - | 198 | WP_153045293 | hypothetical protein | - |
| SAMCFNEI73_RS18310 (SAMCFNEI73_pA0101) | 95819..96526 | - | 708 | WP_064255159 | type IV secretion system protein | - |
| SAMCFNEI73_RS18315 (SAMCFNEI73_pA0102) | 96538..97626 | - | 1089 | WP_064255160 | lytic transglycosylase domain-containing protein | - |
| SAMCFNEI73_RS18320 (SAMCFNEI73_pA0103) | 97623..100082 | - | 2460 | WP_064255161 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| SAMCFNEI73_RS18325 (SAMCFNEI73_pA0104) | 100085..100384 | - | 300 | WP_014531077 | VirB3 family type IV secretion system protein | virB3 |
| SAMCFNEI73_RS18330 (SAMCFNEI73_pA0105) | 100388..100726 | - | 339 | WP_064255162 | TrbC/VirB2 family protein | virB2 |
| SAMCFNEI73_RS18335 (SAMCFNEI73_pA0106) | 100738..101619 | - | 882 | WP_064255169 | lytic transglycosylase domain-containing protein | - |
| SAMCFNEI73_RS18340 (SAMCFNEI73_pA0107) | 101842..102450 | + | 609 | WP_027997197 | hypothetical protein | - |
| SAMCFNEI73_RS18345 (SAMCFNEI73_pA0108) | 102447..102983 | + | 537 | WP_064255163 | hypothetical protein | - |
| SAMCFNEI73_RS18350 (SAMCFNEI73_pA0109) | 102980..103681 | + | 702 | WP_064255164 | thermonuclease family protein | - |
| SAMCFNEI73_RS18360 | 104088..104681 | + | 594 | WP_064255165 | hypothetical protein | - |
| SAMCFNEI73_RS18370 (SAMCFNEI73_pA0111) | 105181..105510 | - | 330 | WP_064255167 | hypothetical protein | - |
Host bacterium
| ID | 4273 | GenBank | NZ_CP013108 |
| Plasmid name | CFNEI 73|A | Incompatibility group | - |
| Plasmid size | 222756 bp | Coordinate of oriT [Strand] | 44950..45006 [-] |
| Host baterium | Sinorhizobium americanum strain CFNEI 73 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |