Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103832
• Name: oriT_p_IPSP_MA_2
• Organism: Variovorax paradoxus strain IPSP_MA_2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP091717 (30038..30136 [-], 99 nt)
• oriT length: 99 nt
• IRs (inverted repeats): 24..31, 34..41 (TAACCGGC..GCCGGTTA)
• Location of nic site: 60..61
• Conserved sequence flanking the nic site: TCCTGCCCGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 99 nt

>oriT_p_IPSP_MA_2
GAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_L3V85_RS37255_p_IPSP_MA_2

• ID: 2842
• GenBank: WP_011342947
• Name: Relaxase_L3V85_RS37255_p_IPSP_MA_2
• UniProt ID: A0A077KSK4
• Length: 746 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 746 a.a.
• Molecular weight: 82038.71 Da
• Isoelectric Point: 10.7900

>WP_011342947.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTNTRAKGDKTYH
LIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIAINKIHPTRHTMHEPYYPHRA
LAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVESLVGWIKRECLDEIKGAQSWQELHQVMRDN
GMELRVRANGLVFEAGDGTMVKASTVARDLSKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTV
ELYAKYKAEQQSLTTARAQALERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSE
IQAINKQFQQERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA
VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEFKAQMIRAAVD
SQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPAADQHATGAAAVARAGDGRPA
AGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRALSELGVVRIAGGSEVLLPRDVPRHVEQQGTQ
PDHALRRGISRPGTGVGQTPPGVAAADKYIAEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALA
LLKRGDEVMVMPIDQATARRLTRIAVGDAVSITAKGSIKTSKGRSR

Protein domains

Predicted by InterproScan.

• Large polyvalent protein-associated domain 7 (LPD7) (422-508)

• Relaxase/Mobilisation nuclease domain (Relaxase) (12-251)

Protein structure

• AlphaFold DB: A0A077KSK4

T4CP

t4cp2_L3V85_RS37260_p_IPSP_MA_2

• ID: 2666
• GenBank: WP_000694765
• Name: t4cp2_L3V85_RS37260_p_IPSP_MA_2
• UniProt ID: _
• Length: 637 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 637 a.a.
• Molecular weight: 69897.09 Da
• Isoelectric Point: 8.2993

>WP_000694765.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Bacteria]
MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHVYAPWSILHWT
YKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNV
LEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGASSVITDLK
GELWALTAGWRQKHAKNKVLRFEPASTSGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQ
KTAFALLVGVILHALYKAKDDGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN
MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRET
GYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLT
PDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPLYFKDPVFSARAAIPAPKVSDRLRAVAQAET
EGEGITI

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (121-620)

Protein structure

No available structure.

Host bacterium

• ID: 4272
• GenBank: NZ_CP091717
• Plasmid name: p_IPSP_MA_2
• Incompatibility group: -
• Plasmid size: 44932 bp
• Coordinate of oriT [Strand]: 30038..30136 [-]
• Host baterium: Variovorax paradoxus strain IPSP_MA_2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: merR2, merP, merD, merE
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -