Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   103818
Name   oriT_pOXA48_MV-u1-SK2-O-b in_silico
Organism   Klebsiella pneumoniae strain MV-u1-SK2-O
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP085868 (40138..40243 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pOXA48_MV-u1-SK2-O-b
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   2834 GenBank   WP_004187323
Name   Relaxase_LKZ97_RS27400_pOXA48_MV-u1-SK2-O-b insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP


ID   2659 GenBank   WP_004206885
Name   t4cp2_LKZ97_RS27105_pOXA48_MV-u1-SK2-O-b insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 43039..62328

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ97_RS27380 (LKZ97_27430) 38123..38434 + 312 WP_004187333 hypothetical protein -
LKZ97_RS27385 (LKZ97_27435) 38567..39103 + 537 WP_004187332 hypothetical protein -
LKZ97_RS27390 (LKZ97_27440) 39605..40000 - 396 WP_019725163 hypothetical protein -
LKZ97_RS27395 (LKZ97_27445) 40035..40562 + 528 WP_172740549 plasmid mobilization protein MobA -
LKZ97_RS27400 (LKZ97_27450) 40549..42528 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
LKZ97_RS27405 (LKZ97_27455) 42542..43042 + 501 WP_004187320 DotD/TraH family lipoprotein -
LKZ97_RS27410 (LKZ97_27460) 43039..43818 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
LKZ97_RS27415 (LKZ97_27465) 43829..44992 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
LKZ97_RS27420 (LKZ97_27470) 44982..45242 + 261 WP_004187310 IcmT/TraK family protein traK
LKZ97_RS27425 (LKZ97_27475) 45267..48704 + 3438 WP_015586048 LPD7 domain-containing protein -
LKZ97_RS27430 (LKZ97_27480) 48670..49182 + 513 WP_011091071 hypothetical protein traL
LKZ97_RS27435 (LKZ97_27485) 49183..49395 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
LKZ97_RS27440 (LKZ97_27490) 49367..49777 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
LKZ97_RS27445 (LKZ97_27495) 49845..50558 + 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
LKZ97_RS27450 (LKZ97_27500) 50567..51718 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
LKZ97_RS27455 (LKZ97_27505) 51730..53079 + 1350 WP_004187474 conjugal transfer protein TraO traO
LKZ97_RS27460 (LKZ97_27510) 53091..53795 + 705 WP_015060002 conjugal transfer protein TraP traP
LKZ97_RS27465 (LKZ97_27515) 53819..54349 + 531 WP_004187478 conjugal transfer protein TraQ traQ
LKZ97_RS27470 (LKZ97_27520) 54366..54755 + 390 WP_004187479 DUF6750 family protein traR
LKZ97_RS27475 (LKZ97_27525) 54801..55295 + 495 WP_004187480 hypothetical protein -
LKZ97_RS27480 (LKZ97_27530) 55292..58342 + 3051 WP_004187482 hypothetical protein traU
LKZ97_RS27485 (LKZ97_27535) 58339..59547 + 1209 WP_011091082 conjugal transfer protein TraW traW
LKZ97_RS27490 (LKZ97_27540) 59544..60140 + 597 WP_015060003 hypothetical protein -
LKZ97_RS27495 (LKZ97_27545) 60133..62328 + 2196 WP_015062834 DotA/TraY family protein traY
LKZ97_RS27500 (LKZ97_27550) 62330..62983 + 654 WP_015060005 hypothetical protein -
LKZ97_RS27505 (LKZ97_27555) 63064..63294 + 231 WP_011091085 IncL/M type plasmid replication protein RepC -


Host bacterium


ID   4258 GenBank   NZ_CP085868
Plasmid name   pOXA48_MV-u1-SK2-O-b Incompatibility group   IncL/M
Plasmid size   63589 bp Coordinate of oriT [Strand]   40138..40243 [+]
Host baterium   Klebsiella pneumoniae strain MV-u1-SK2-O

Cargo genes


Drug resistance gene   blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -