Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103812
• Name: oriT_pSymB
• Organism: Sinorhizobium meliloti strain RMO17
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP009146 (1005035..1005063 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSymB
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_DU99_RS28875_pSymB

• ID: 2830
• GenBank: WP_040121023
• Name: traA_DU99_RS28875_pSymB
• UniProt ID: _
• Length: 1539 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1539 a.a.
• Molecular weight: 170023.69 Da
• Isoelectric Point: 9.0744

>WP_040121023.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium meliloti]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRTRMIDEQAGTSFSYRGGASELVHEELALPDDIPAWLKAAIDG
RSVAKASEALWNAVEAHETRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTEEGFGPKKVPVLGEDGEPLRVITPDRPNGKIVYKLWAGDKETIKAWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIAMYFAPADLARRQEMADRLLAEPELLLKQ
LGNERSTFDERDIAKALHRYVDDPVDFANIRARLMASDELVLLKPQQVDAETGKAKQPAVFTTREMLRLE
YAMARSAEVLSRRKGFGVSNARAAAAVRSIETADTEKPFRLDPEQVDAVRHVTRDNAIAAVVGLAGAGKS
TLLAAARVAWEGEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELAWESGCEQLQRGDVLVIDEAGMVS
SQQMARILKAVEDAGAKAVLVGDAMQLQPIEAGAAFRAITERIGFAELAGVRRQRDAWARDASRLFARGK
VEEGLDAYAQQGRIVETETRAEIVDRIVADWANARRDLLQKSADGEHPGRLRGDELLVLAHTNDDVRKLN
EALRQVMIGEGALTGAREFQTARGLREFAAGDRIIFLENARFVEPRARRLGPQYVKNGMLGTVVSTGDRR
GDTLLSVRLDSGRDVVISEDSYRNVDHGYAATIHKSQGSTVDRTFVLATGMMDQHLTYVAMTRHRDRADL
YAAKEDFEAKPEWGRKPRVDHAAGVTGELVEEGMAKFRPNDEDADESPYADIRTDDGTVHRLWGVSLPKA
LKDAGAAEGDTITLRKDGVERVKVQVPIVDEQTGEKRFEERQVDRNVWSASQLETAAARRERIERESHRP
QLFAALVERLSRSGAKTTTLDFEDEAGYQAQARDFARRRGLYHLSLVAAGMEAEVLRRWAGIAEKREQVA
KLWERASVALGFAIERERRVSYNEERTETLSTGIPSDGKYLVPPTTTFSRSVAEDARLAQLSSQRWKERE
AILHPVLAKIYRDPDGALSALNALASDAAIEPRKLADDLGKAPDRLGRLRGSELVVDGRAARDERTAATV
ALSELLPLARAHATEFRRNAERFGIREQQRRAHMALSVPALSKTAMARLVEIEAVRKQGGDDAYRTAFAF
AVEDRLLVQEVKAVNEALTARFGWSAFTAKADVIAERNIAERMPEDLAPERRERLTRLFAVIRRFAEEQH
LAERQERSKIVAGASVELGKGTFAVLPMLAPVTEFKTTVDEEARERALAAPHYAHHRAALVETATRVWRD
PADAIGKIEDLIVKGFAAERIAAAVTNDPAAYGALRGSDRIMDKLLAAGRERKDALQAVPEAASRIRSLG
ASYASALDAETRSITEERRRMAVAIPGLSPAAEDALKRLAAQIKNKDGKLDVAAGSLDPHIAREFAKVSR
ALDERFGRNAILRGETDVINRVSPAQRRAFEAMRDRLQVLQQAVRIQSSQKIVSERRQRAINQPRGIRM

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1125)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1309-1400)

• AAA domain (AAA_30) (391-578)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

T4CP

tcpA_DU99_RS35370_pSymB

• ID: 2655
• GenBank: WP_027988248
• Name: tcpA_DU99_RS35370_pSymB
• UniProt ID: _
• Length: 946 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 946 a.a.
• Molecular weight: 101647.44 Da
• Isoelectric Point: 4.5919

>WP_027988248.1 FtsK/SpoIIIE domain-containing protein [Sinorhizobium meliloti]
MRIPRTNFSPAALYDANHADDYEDPHPAAQGAAHVPAAPEAAAHDHLFEAPEAPRRAPGHRDEETGYAGR
AARLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSGEPGWESHFFLSPNVRFTRTPERELMKRHPPAPEES
RIEADEAAAEASDAETVMDVVPAEPAPSVVETELPSYSPSELLRVLTQQLPSWSAARSQALEASVTKPAI
TESVAVAEEGPATSETTALPVTDEVPVVPHALPVANLAPESAAVEEDVPHQADARLAYLSDFAFFEFMPL
EVAAVPPTVTEPVKEAARIPAPIAAAPKVSPPKIVAAMPVEIRPQPPTAISSLFRVVECRRPEPATAEPV
TAAPQDIAAEPAAAEAAALPAHQFVETPAPALAEPAIVPASEPAPEAPVTRAAITMPAVIQRSSPSLPPI
GAIEPLQGGDAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVVTLYEF
EPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTGCKLAL
CLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSVYDGIP
HLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGAPILATVQTGFEKGTGE
PLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKA
NFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKTQGRPE
YLETVTADEEEEEVEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRAASLVE
RMEKDGLVGPANHVGKREIIYGNRDNPPKPESDDLD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (543-761)

• Ftsk gamma domain (FtsK_gamma) (871-930)

• FtsK alpha domain (FtsK_alpha) (434-534)

Protein structure

No available structure.

Host bacterium

• ID: 4252
• GenBank: NZ_CP009146
• Plasmid name: pSymB
• Incompatibility group: -
• Plasmid size: 1617037 bp
• Coordinate of oriT [Strand]: 1005035..1005063 [-]
• Host baterium: Sinorhizobium meliloti strain RMO17

Cargo genes

• Drug resistance gene: -
• Virulence gene: htpB
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA7