Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103763
• Name: oriT_B594|p2
• Organism: Enterococcus faecalis EnGen0107 strain B594
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP041740 (22786..22823 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_B594|p2
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_SAW_RS17150_B594|p2

• ID: 2795
• GenBank: WP_000119405
• Name: mobV_SAW_RS17150_B594|p2
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

T4CP

t4cp2_SAW_RS17050_B594|p2

• ID: 2622
• GenBank: WP_010714316
• Name: t4cp2_SAW_RS17050_B594|p2
• UniProt ID: _
• Length: 454 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 454 a.a.
• Molecular weight: 52487.99 Da
• Isoelectric Point: 4.5777

>WP_010714316.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQQNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLLNTLIKYVYFEYEPSARTIEGILDFLEE
FDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARPNIVISLLTTLQDFVDKEVSEFTSTNDF
FFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGDKHNAKLPVPLVMLLDEFVNLGYFPTYE
NFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKICLGGVEETTAEYFSRQVNDTTIKVYTG
GTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEENGRKSIVLIDGKPYMLRKTPQFELFGNL
LKKHEISQQDYISSQTEFAKETIEELEKQHKQRKVQLASAPIFHEKKQEDDLNKKEINLPEQPIESEVTE
EEEEKELLMSDILSSFDEGIEENTTNNDNSELPF

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (3-359)

Protein structure

No available structure.

t4cp2_SAW_RS17070_B594|p2

• ID: 2623
• GenBank: WP_000209436
• Name: t4cp2_SAW_RS17070_B594|p2
• UniProt ID: _
• Length: 546 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 546 a.a.
• Molecular weight: 62672.60 Da
• Isoelectric Point: 7.6569

>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (3-462)

Protein structure

No available structure.

Host bacterium

• ID: 4203
• GenBank: NZ_CP041740
• Plasmid name: B594|p2
• Incompatibility group: -
• Plasmid size: 45436 bp
• Coordinate of oriT [Strand]: 22786..22823 [-]
• Host baterium: Enterococcus faecalis EnGen0107 strain B594

Cargo genes

• Drug resistance gene: aac(6')-aph(2''), aadD, erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -