Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103762
• Name: oriT_B594|p1
• Organism: Enterococcus faecalis EnGen0107 strain B594
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP041739 (16046..16095 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_B594|p1
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_SAW_RS16680_B594|p1

• ID: 2794
• GenBank: WP_010714222
• Name: Relaxase_SAW_RS16680_B594|p1
• UniProt ID: A0A855UBV4
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65338.96 Da
• Isoelectric Point: 6.4206

>WP_010714222.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSKLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETTNQNNVKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

• AlphaFold DB: A0A855UBV4

Auxiliary protein

WP_002393737_B594|p1

• ID: 1178
• GenBank: WP_002393737
• Name: WP_002393737_B594|p1
• UniProt ID: A0A8E7CBI6
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13965.01 Da
• Isoelectric Point: 9.6545

>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_SAW_RS16660_B594|p1

• ID: 2621
• GenBank: WP_002393740
• Name: t4cp2_SAW_RS16660_B594|p1
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70145.09 Da
• Isoelectric Point: 6.0617

>WP_002393740.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGQELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

Host bacterium

• ID: 4202
• GenBank: NZ_CP041739
• Plasmid name: B594|p1
• Incompatibility group: -
• Plasmid size: 70182 bp
• Coordinate of oriT [Strand]: 16046..16095 [+]
• Host baterium: Enterococcus faecalis EnGen0107 strain B594

Cargo genes

• Drug resistance gene: -
• Virulence gene: asa1
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9, AcrIIA21