Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103748 |
| Name | oriT_pSARA30  |
| Organism | Salmonella enterica subsp. enterica serovar Heidelberg str. SARA30 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NC_019138 (33284..33890 [+], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..202, 204..210 (TCAATTC..GAATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_pSARA30
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2778 | GenBank | WP_000539532 |
| Name | Relaxase_HTA16_RS00175_pSARA30 |
UniProt ID | K4NPN4 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44348.73 Da Isoelectric Point: 10.5588
>WP_000539532.1 MULTISPECIES: MobP1 family relaxase [Gammaproteobacteria]
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GGLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | K4NPN4 |
T4CP
| ID | 2606 | GenBank | WP_000053816 |
| Name | t4cp2_HTA16_RS00110_pSARA30 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69371.31 Da Isoelectric Point: 8.0636
>WP_000053816.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Gammaproteobacteria]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTAVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKNRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTAVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKNRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20792..30744
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTA16_RS00090 (pSARA30_14) | 15802..16008 | - | 207 | WP_000850859 | hemolysin expression modulator Hha | - |
| HTA16_RS00095 (pSARA30_15) | 16005..18158 | - | 2154 | WP_001235737 | type IA DNA topoisomerase | - |
| HTA16_RS00100 (pSARA30_16) | 18245..18547 | - | 303 | WP_000820909 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| HTA16_RS00105 (pSARA30_17) | 18544..18957 | - | 414 | WP_000722610 | cag pathogenicity island Cag12 family protein | - |
| HTA16_RS00110 (pSARA30_18) | 18954..20789 | - | 1836 | WP_000053816 | type IV secretory system conjugative DNA transfer family protein | - |
| HTA16_RS00115 (pSARA30_19) | 20792..21823 | - | 1032 | WP_001058474 | P-type DNA transfer ATPase VirB11 | virB11 |
| HTA16_RS00120 (pSARA30_20) | 21825..23030 | - | 1206 | WP_000139138 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| HTA16_RS00125 (pSARA30_21) | 23027..23956 | - | 930 | WP_000783379 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| HTA16_RS00130 (pSARA30_22) | 23961..24674 | - | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| HTA16_RS00135 (pSARA30_24) | 24919..25980 | - | 1062 | WP_000796671 | type IV secretion system protein | virB6 |
| HTA16_RS00140 (pSARA30_25) | 25992..26276 | - | 285 | WP_000748128 | EexN family lipoprotein | - |
| HTA16_RS00145 (pSARA30_26) | 26291..27040 | - | 750 | WP_000744206 | type IV secretion system protein | - |
| HTA16_RS00150 (pSARA30_27) | 27051..29804 | - | 2754 | WP_001532172 | VirB3 family type IV secretion system protein | virb4 |
| HTA16_RS00155 (pSARA30_28) | 29823..30119 | - | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| HTA16_RS00160 (pSARA30_29) | 30097..30744 | - | 648 | WP_000953530 | lytic transglycosylase domain-containing protein | virB1 |
| HTA16_RS00165 | 30777..30989 | - | 213 | WP_000125172 | hypothetical protein | - |
| HTA16_RS00170 (pSARA30_30) | 30919..31437 | - | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| HTA16_RS00175 (pSARA30_31) | 31790..32956 | - | 1167 | WP_000539532 | MobP1 family relaxase | - |
| HTA16_RS00180 (pSARA30_32) | 32959..33504 | - | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| HTA16_RS00190 (pSARA30_33) | 33830..34102 | - | 273 | WP_000160399 | hypothetical protein | - |
| HTA16_RS00195 (pSARA30_34) | 34115..34312 | - | 198 | WP_001675595 | hypothetical protein | - |
| HTA16_RS00200 (pSARA30_35) | 34334..34513 | - | 180 | WP_000439078 | hypothetical protein | - |
| HTA16_RS00205 (pSARA30_36) | 34556..34885 | - | 330 | WP_000866646 | hypothetical protein | - |
| HTA16_RS00210 (pSARA30_37) | 34978..35193 | - | 216 | WP_001180116 | hypothetical protein | - |
| HTA16_RS00215 (pSARA30_38) | 35183..35428 | - | 246 | WP_000356547 | hypothetical protein | - |
Host bacterium
| ID | 4188 | GenBank | NC_019138 |
| Plasmid name | pSARA30 | Incompatibility group | IncX1 |
| Plasmid size | 37697 bp | Coordinate of oriT [Strand] | 33284..33890 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Heidelberg str. SARA30 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |