Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103747
Name   oriT_pSNE1-2010K-2159 in_silico
Organism   Salmonella enterica subsp. enterica serovar Newport str. CDC 2010K-2159
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP025255 (68879..68958 [-], 80 nt)
oriT length   80 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 80 nt

>oriT_pSNE1-2010K-2159
GGGTGTCGGGGCGAAGCCCTGACCAGGTGGTAAATGTATGGCCGTGCGTGCGCGGTTATACATTTACACATCCTGTCCCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2777 GenBank   WP_168445504
Name   nikB_AW90_RS00415_pSNE1-2010K-2159 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104590.17 Da        Isoelectric Point: 7.3254

>WP_168445504.1 IncI1-type relaxase NikB [Salmonella enterica]
MNAIIPKKRRDGKSSFEDLVSYVSVRDDLEDEELQALSSVQSELSHRSRFSRLVDYATRLRNESFVSLVD
VMKDGCEWGNFYGVTCFHNCTSLETAAEEMEYTARQARYAKDDTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKALGLSEHQYVSAVHTDTDNLHVHVAVNRVHPDTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
IHAPGSRIVRKTAAERDRQNAWTRGKKQTFREYVSQMAVAGLRSEPVHDWLSLHRRLAEDGLYLTRQNGS
FMVMDGWDRNREGVQLDSFGPSWNGDKLRKKMGEYTPVPKDIFSQVGAPGRYKPEVIAADTRPEKMAETM
NLMQYACRHLGDRLPEMAREGKLESCLDIHRILAEAGLWMRIQHGHLVICDGYDHNQTPVRADSVWPLLT
PENINQLDGGWQPVPDDIFRQVTPAERFSGRRLESCPASDREWHRMRTGPGPQGAIRRELFSDKESLWGY
SISHCSPQIEEMITRGEFTWHHCHELFARQGLMLQRQHHGLVVVDAFNHEQTPVKASSIHPDLTLERAEP
QAGPFVSAPADLFDRVKPESRYNPELAVSDQSREDCRRDPVLRRQRREARAEARADLRARYLAWREQWRK
PELRYGERSREIHQACRLRKSHIRVQYDDPVLRKLHYHIAEVQRMQALIRLKEEVRNERQKLIAEGKWYP
PSYRQWVEAQAVQGDRAAVSQLRGWDYRDRRKDKSRTTTADRCVILCEPGGTPVYENRGELEARLQKNGS
VRFRDRRTDQFVCTDYGDRVVFHNHHDRNELADKLDLIAPVLFERDPRMGFEPEGNDRQFNQVFAEMVAW
HNVTERTGHGDYTISRPDVDHHREGSERYYRDYINAHTGREASLPSLEHEKRHEPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   1175 GenBank   WP_001281049
Name   WP_001281049_pSNE1-2010K-2159 insolico UniProt ID   A0A5T6J7U7
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12698.63 Da        Isoelectric Point: 10.6858

>WP_001281049.1 plasmid mobilization protein MobA [Salmonella enterica]
MSDKRERSGSEKRQKTLLRAVRFSPDEDEIIRKKAEDAGLTVSAYIRSAALNKRVNSRIDDKFLKELMRL
GRMQKHLFVEGKRTGDKEYASVLVAITELSNTVRKQLMEN

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5T6J7U7


T4CP

ID   2605 GenBank   WP_168445505
Name   trbC_AW90_RS00420_pSNE1-2010K-2159 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 87008.20 Da        Isoelectric Point: 7.6442

>WP_168445505.1 F-type conjugative transfer protein TrbC [Salmonella enterica]
MSEHRVNPDLIHRTAWGNPLWNALQNLNIYGMCLAGNLVVSFIWPLALPACLLFTLITVLIFSLQRWRCP
LRMPMTLRCADPSQDRMIKRSLFEFWPTLFQYETIKEFPANGIFYVGYQRIRDIGRELWLNIDDLTRHVM
FFAATGGGKTETIFSWIINPLCWGRGFTLVDGKAQNDTARTIWYLSRRFGREDDVEVINFMNGGKSRSEI
ILSGDKSRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGRTMSL
QMLREHMSLEGMAKLYCRGIDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFVTQQSMILARDLGYRLE
GSDSQALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTSATSTATLM
QNTGTKFAGRVVSDDKTARTLKNAAGEEARARMNSLQRQDGVFGEAWIDSPQISILMESKVNVQELIKLH
PGEFFSIFQGESVPAASFYIPDEEKSCSSDPVVINRYISVDAPRLEQLRRLVPRTVQRRIPSPENVSAII
GVLTAKPSRKRRKKRTEPHIIVDTFQQRLAGRQAAMALLEEYDTDINAREMALWETALNTLKTTTREQRR
IRFITLNRPETPAADEEHQVSVRAERAEINLLSLPKQNYRPAGPSANGSHHKNNSRHEWKGLY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74338..110401

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW90_RS00420 (AW90_50065) 72054..74345 - 2292 WP_168445505 F-type conjugative transfer protein TrbC -
AW90_RS00425 (AW90_50070) 74338..75414 - 1077 WP_168445506 DsbC family protein trbB
AW90_RS00430 (AW90_50075) 75432..76640 - 1209 WP_115396628 IncI1-type conjugal transfer protein TrbA trbA
AW90_RS00435 (AW90_50080) 76788..77132 + 345 WP_168445507 toxin -
AW90_RS00440 (AW90_50085) 77125..77439 + 315 WP_000166615 type II toxin-antitoxin system MqsA family antitoxin -
AW90_RS00445 (AW90_50090) 77607..77765 + 159 WP_115396658 Hok/Gef family protein -
AW90_RS00450 (AW90_50095) 78337..78786 + 450 WP_115396627 hypothetical protein -
AW90_RS00455 (AW90_50100) 79168..79797 - 630 WP_237228164 plasmid IncI1-type surface exclusion protein ExcA -
AW90_RS00460 (AW90_50105) 79857..82028 - 2172 WP_115396625 DotA/TraY family protein traY
AW90_RS00465 (AW90_50110) 82127..82711 - 585 WP_168445508 conjugal transfer protein TraX -
AW90_RS00470 (AW90_50115) 82704..82955 - 252 WP_168445509 hypothetical protein -
AW90_RS00475 (AW90_50120) 82952..84154 - 1203 WP_023180535 conjugal transfer protein TraW traW
AW90_RS00480 (AW90_50125) 84112..84741 - 630 WP_115396624 conjugal transfer protein TraV -
AW90_RS00485 (AW90_50130) 84741..87785 - 3045 WP_115396623 ATP-binding protein traU
AW90_RS00490 (AW90_50135) 87883..88683 - 801 WP_115396622 conjugal transfer protein TraT traT
AW90_RS00500 (AW90_50145) 88924..89328 - 405 WP_000088876 DUF6750 family protein traR
AW90_RS00505 (AW90_50150) 89371..89898 - 528 WP_001090609 conjugal transfer protein TraQ traQ
AW90_RS00510 (AW90_50155) 89898..90611 - 714 WP_115396621 conjugal transfer protein TraP traP
AW90_RS00515 (AW90_50160) 90608..91909 - 1302 WP_115396620 conjugal transfer protein TraO traO
AW90_RS00520 (AW90_50165) 91912..92895 - 984 WP_023180540 DotH/IcmK family type IV secretion protein traN
AW90_RS00525 (AW90_50170) 92906..93598 - 693 WP_168445511 DotI/IcmL family type IV secretion protein traM
AW90_RS00530 (AW90_50175) 93610..93960 - 351 WP_115396619 conjugal transfer protein traL
AW90_RS00535 (AW90_50180) 93978..97595 - 3618 WP_168445512 LPD7 domain-containing protein -
AW90_RS00540 (AW90_50185) 97876..98433 - 558 WP_168445513 phospholipase D family protein -
AW90_RS00545 (AW90_50190) 98448..98738 - 291 WP_001838506 hypothetical protein traK
AW90_RS00550 (AW90_50195) 98735..99883 - 1149 WP_115396616 plasmid transfer ATPase TraJ virB11
AW90_RS00555 (AW90_50200) 100027..100860 - 834 WP_000866092 type IV secretory system conjugative DNA transfer family protein traI
AW90_RS00560 (AW90_50205) 100857..101312 - 456 WP_115396615 DotD/TraH family lipoprotein -
AW90_RS00565 (AW90_50210) 101829..103031 - 1203 WP_168445514 conjugal transfer protein TraF -
AW90_RS00570 (AW90_50215) 103118..103942 - 825 Protein_113 conjugal transfer protein TraE -
AW90_RS00575 (AW90_50220) 104093..104416 - 324 Protein_114 integrase -
AW90_RS00580 (AW90_50225) 104564..105892 - 1329 WP_060634146 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
AW90_RS00585 (AW90_50230) 105892..106548 - 657 WP_115396612 prepilin peptidase -
AW90_RS00590 (AW90_50235) 106533..107093 - 561 WP_000012997 lytic transglycosylase domain-containing protein virB1
AW90_RS00595 (AW90_50240) 107103..107720 - 618 WP_000470555 type 4 pilus major pilin -
AW90_RS00600 (AW90_50245) 107738..108835 - 1098 WP_168445515 type II secretion system F family protein -
AW90_RS00605 (AW90_50250) 108848..110401 - 1554 WP_115396610 ATPase, T2SS/T4P/T4SS family virB11
AW90_RS00610 (AW90_50255) 110412..110864 - 453 WP_115396609 type IV pilus biogenesis protein PilP -
AW90_RS00615 (AW90_50260) 110851..112152 - 1302 WP_168445516 type 4b pilus protein PilO2 -
AW90_RS00620 (AW90_50265) 112145..113827 - 1683 WP_000733117 PilN family type IVB pilus formation outer membrane protein -
AW90_RS00625 (AW90_50270) 113841..114278 - 438 WP_115396607 type IV pilus biogenesis protein PilM -
AW90_RS00630 (AW90_50275) 114278..115345 - 1068 WP_115396606 TcpQ domain-containing protein -

Host bacterium

ID   4187 GenBank   NZ_CP025255
Plasmid name   pSNE1-2010K-2159 Incompatibility group   IncI1
Plasmid size   119751 bp Coordinate of oriT [Strand]   68879..68958 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Newport str. CDC 2010K-2159

Cargo genes

Drug resistance gene   -
Virulence gene   pic
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -