Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103745
Name   oriT_pSAN1-08-1092 in_silico
Organism   Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1781
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP019996 (30714..30802 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pSAN1-08-1092
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2773 GenBank   WP_071447698
Name   nikB_AW61_RS00235_pSAN1-08-1092 insolico UniProt ID   A0A1S7B047
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103868.35 Da        Isoelectric Point: 7.4198

>WP_071447698.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMEGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S7B047


Auxiliary protein

ID   1174 GenBank   WP_001283947
Name   WP_001283947_pSAN1-08-1092 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   2602 GenBank   WP_001289275
Name   trbC_AW61_RS00240_pSAN1-08-1092 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86957.15 Da        Isoelectric Point: 6.7713

>WP_001289275.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 36180..76425

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW61_RS00240 (AW61_23985) 33896..36187 - 2292 WP_001289275 F-type conjugative transfer protein TrbC -
AW61_RS00245 (AW61_23990) 36180..37250 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
AW61_RS00250 (AW61_23995) 37269..38477 - 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
AW61_RS00255 (AW61_24000) 38695..39681 - 987 WP_001257838 hypothetical protein -
AW61_RS00260 (AW61_24005) 39954..40106 + 153 WP_001331364 Hok/Gef family protein -
AW61_RS00265 (AW61_24010) 40178..40429 - 252 WP_001291964 hypothetical protein -
AW61_RS00515 40928..41023 + 96 WP_001303310 DinQ-like type I toxin DqlB -
AW61_RS00270 41088..41372 - 285 WP_014640317 hypothetical protein -
AW61_RS00275 (AW61_24015) 41596..41805 + 210 WP_000062602 HEAT repeat domain-containing protein -
AW61_RS00280 (AW61_24020) 41877..42539 - 663 WP_000644792 plasmid IncI1-type surface exclusion protein ExcA -
AW61_RS00285 (AW61_24025) 42604..44772 - 2169 WP_000698366 DotA/TraY family protein traY
AW61_RS00290 (AW61_24030) 44869..45453 - 585 WP_001037996 IncI1-type conjugal transfer protein TraX -
AW61_RS00295 (AW61_24035) 45482..46684 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
AW61_RS00300 (AW61_24040) 46651..47265 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
AW61_RS00305 (AW61_24045) 47265..50309 - 3045 WP_001024776 IncI1-type conjugal transfer protein TraU traU
AW61_RS00310 (AW61_24050) 50399..51199 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
AW61_RS00315 (AW61_24055) 51183..51371 - 189 WP_001277255 putative conjugal transfer protein TraS -
AW61_RS00320 (AW61_24060) 51435..51839 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
AW61_RS00325 (AW61_24065) 51890..52417 - 528 WP_001055569 conjugal transfer protein TraQ traQ
AW61_RS00330 (AW61_24070) 52417..53121 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
AW61_RS00335 (AW61_24075) 53121..54410 - 1290 WP_001271994 conjugal transfer protein TraO traO
AW61_RS00340 (AW61_24080) 54413..55396 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
AW61_RS00345 (AW61_24085) 55407..56099 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
AW61_RS00350 (AW61_24090) 56096..56443 - 348 WP_001055900 conjugal transfer protein traL
AW61_RS00355 (AW61_24095) 56461..60228 - 3768 WP_057517056 LPD7 domain-containing protein -
AW61_RS00360 (AW61_24100) 60318..60869 - 552 WP_000014584 phospholipase D family protein -
AW61_RS00365 (AW61_24105) 60884..61174 - 291 WP_001299214 hypothetical protein traK
AW61_RS00370 (AW61_24110) 61171..62319 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
AW61_RS00375 (AW61_24115) 62316..63134 - 819 WP_000866663 IncI1-type conjugal transfer lipoprotein TraI traI
AW61_RS00380 (AW61_24120) 63131..63589 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
AW61_RS00385 (AW61_24125) 63984..64568 - 585 WP_000977520 histidine phosphatase family protein -
AW61_RS00390 (AW61_24130) 64628..65830 - 1203 WP_000976357 conjugal transfer protein TraF -
AW61_RS00395 (AW61_24135) 65916..66740 - 825 WP_057517055 conjugal transfer protein TraE traE
AW61_RS00400 (AW61_24140) 66891..68045 - 1155 WP_001139955 site-specific integrase -
AW61_RS00405 (AW61_24145) 68172..68327 + 156 WP_001302705 hypothetical protein -
AW61_RS00410 (AW61_24160) 68640..68897 + 258 WP_001302706 hypothetical protein -
AW61_RS00415 (AW61_24170) 69479..69736 - 258 WP_001302706 hypothetical protein -
AW61_RS00420 (AW61_24180) 69864..70019 + 156 WP_001302705 hypothetical protein -
AW61_RS00425 70395..70643 + 249 WP_001349157 hypothetical protein -
AW61_RS00430 (AW61_24195) 70640..71932 - 1293 WP_039719938 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
AW61_RS00435 (AW61_24200) 71932..72588 - 657 WP_001193551 A24 family peptidase -
AW61_RS00440 (AW61_24205) 72573..73133 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
AW61_RS00445 (AW61_24210) 73143..73757 - 615 WP_000908228 type 4 pilus major pilin -
AW61_RS00450 (AW61_24215) 73774..74859 - 1086 WP_057517061 type II secretion system F family protein -
AW61_RS00455 (AW61_24220) 74872..76425 - 1554 WP_000362204 ATPase, T2SS/T4P/T4SS family virB11
AW61_RS00460 (AW61_24225) 76436..76888 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
AW61_RS00465 (AW61_24230) 76875..78170 - 1296 WP_000752776 type 4b pilus protein PilO2 -
AW61_RS00470 (AW61_24235) 78163..79845 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
AW61_RS00475 (AW61_24240) 79859..80296 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
AW61_RS00480 (AW61_24245) 80296..81363 - 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   4185 GenBank   NZ_CP019996
Plasmid name   pSAN1-08-1092 Incompatibility group   IncI1
Plasmid size   85439 bp Coordinate of oriT [Strand]   30714..30802 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Anatum str. USDA-ARS-USMARC-1781

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -