Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103744
• Name: oriT_pACK4
• Organism: Staphylococcus simulans bv. staphylolyticus
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_013033 (1617..1737 [+], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..59, 63..69 (TTGGGAT..ATCCCAA) 25..31, 36..42 (TTTTTGC..GCAAAAA) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_pACK4
AGTGGCTAGCATTTAGCCACTCATTTTTTGCGTGAGCAAAAACATAAAGGGCTTGGGATATAATCCCAACAAGGACGGCGAGCTACCACGTAAGTGGCTAGCAAAGCCAGTGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_HS543_RS00005_pACK4

• ID: 2772
• GenBank: WP_012779610
• Name: Rep_trans_HS543_RS00005_pACK4
• UniProt ID: C6ZNM0
• Length: 312 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 312 a.a.
• Molecular weight: 37654.85 Da
• Isoelectric Point: 8.5902

>WP_012779610.1 replication initiation factor domain-containing protein [Staphylococcus simulans]
MSENIRENHSKQGTSEKVTNYTRTGYSNSRLNRHTMYTPSPKLSFDAMTIVGNLNKNNAQKLSKFMSIEP
QIRLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTR
LDLAFDFEDDLGDYYAITDKAVKKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEVISEHL
WRVEIELKRNMVDFWNDCFNDLHILQPDWKTIERTSDRAMVFMLLNDEEEWGKLERRTKNKYKKIIKEIS
PIDLTEIMKLTLRENEKQLQKQIDFWLSDFRF

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (138-302)

Protein structure

• AlphaFold DB: C6ZNM0

Host bacterium

• ID: 4184
• GenBank: NC_013033
• Plasmid name: pACK4
• Incompatibility group: -
• Plasmid size: 3592 bp
• Coordinate of oriT [Strand]: 1617..1737 [+]
• Host baterium: Staphylococcus simulans bv. staphylolyticus

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -