Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 103740 |
Name | oriT_69-3818|unnamed1 |
Organism | Shigella dysenteriae strain 69-3818 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP026778 (4840..4925 [-], 86 nt) |
oriT length | 86 nt |
IRs (inverted repeats) | _ |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 86 nt
>oriT_69-3818|unnamed1
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTT
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 2770 | GenBank | WP_000991407 |
Name | Relaxase_C5C01_RS24195_69-3818|unnamed1 | UniProt ID | A0A142CND5 |
Length | 906 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 906 a.a. Molecular weight: 104908.50 Da Isoelectric Point: 8.2690
>WP_000991407.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVEAPGRYDPEAINVDIRPEKVAETE
SLKQYASRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVEAPGRYDPEAINVDIRPEKVAETE
SLKQYASRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A142CND5 |
Auxiliary protein
ID | 1171 | GenBank | WP_001291057 |
Name | WP_001291057_69-3818|unnamed1 | UniProt ID | A0A142CND4 |
Length | 110 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 110 a.a. Molecular weight: 12596.69 Da Isoelectric Point: 10.7335
>WP_001291057.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLGKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRNLMGR
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLGKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRNLMGR
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A142CND4 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10505..48391
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
C5C01_RS24200 | 8225..10524 | - | 2300 | Protein_11 | F-type conjugative transfer protein TrbC | - |
C5C01_RS24205 | 10505..11629 | - | 1125 | WP_000152384 | DsbC family protein | trbB |
C5C01_RS27175 | 11626..12951 | - | 1326 | WP_171265269 | IncI1-type conjugal transfer protein TrbA | trbA |
C5C01_RS28935 | 13488..13583 | + | 96 | WP_001363141 | DinQ-like type I toxin DqlB | - |
C5C01_RS28940 | 14172..14267 | + | 96 | WP_001363141 | DinQ-like type I toxin DqlB | - |
C5C01_RS27750 | 14330..14506 | - | 177 | WP_001054907 | hypothetical protein | - |
C5C01_RS24225 | 14657..15124 | - | 468 | WP_000598962 | thermonuclease family protein | - |
C5C01_RS24230 | 15284..15907 | + | 624 | WP_001155250 | helix-turn-helix domain-containing protein | - |
C5C01_RS24240 | 16105..16319 | - | 215 | Protein_19 | hypothetical protein | - |
C5C01_RS24245 | 16323..16691 | - | 369 | WP_001363143 | hypothetical protein | - |
C5C01_RS24255 | 16991..17233 | - | 243 | WP_001363144 | hypothetical protein | - |
C5C01_RS24260 | 17563..17715 | + | 153 | WP_001302699 | Hok/Gef family protein | - |
C5C01_RS24275 | 18341..18988 | - | 648 | WP_015056434 | plasmid IncI1-type surface exclusion protein ExcA | - |
C5C01_RS24280 | 19075..21233 | - | 2159 | Protein_24 | DotA/TraY family protein | - |
C5C01_RS24285 | 21124..21771 | - | 648 | WP_237161441 | conjugal transfer protein TraX | - |
C5C01_RS24290 | 21872..23077 | - | 1206 | WP_001189548 | conjugal transfer protein TraW | traW |
C5C01_RS24300 | 23653..26694 | - | 3042 | Protein_27 | ATP-binding protein | - |
C5C01_RS24310 | 26988..27614 | - | 627 | WP_000785703 | hypothetical protein | traT |
C5C01_RS28945 | 27721..27972 | - | 252 | WP_001277573 | hypothetical protein | - |
C5C01_RS24320 | 28029..28427 | - | 399 | WP_000088813 | DUF6750 family protein | traR |
C5C01_RS24325 | 28474..29004 | - | 531 | WP_000987021 | conjugal transfer protein TraQ | traQ |
C5C01_RS24330 | 29001..29483 | - | 483 | WP_237161442 | conjugal transfer protein TraP | traP |
C5C01_RS28635 | 29480..29713 | - | 234 | WP_237161443 | hypothetical protein | traP |
C5C01_RS24335 | 29710..31043 | - | 1334 | Protein_34 | conjugal transfer protein TraO | - |
C5C01_RS24340 | 31047..32021 | - | 975 | WP_000547385 | DotH/IcmK family type IV secretion protein | traN |
C5C01_RS24345 | 32032..32727 | - | 696 | WP_001287545 | DotI/IcmL family type IV secretion protein | traM |
C5C01_RS24350 | 32739..33089 | - | 351 | WP_001056367 | hypothetical protein | traL |
C5C01_RS24355 | 33106..37167 | - | 4062 | WP_000842453 | LPD7 domain-containing protein | - |
C5C01_RS24360 | 37231..37521 | - | 291 | WP_000817801 | IcmT/TraK family protein | traK |
C5C01_RS24365 | 37518..38664 | - | 1147 | Protein_40 | plasmid transfer ATPase TraJ | - |
C5C01_RS24370 | 38648..39483 | - | 836 | Protein_41 | type IV secretory system conjugative DNA transfer family protein | - |
C5C01_RS24375 | 39480..39938 | - | 459 | WP_001170111 | DotD/TraH family lipoprotein | - |
C5C01_RS24380 | 40042..41244 | - | 1203 | WP_000979993 | conjugal transfer protein TraF | - |
C5C01_RS24385 | 41346..42167 | - | 822 | WP_000845373 | hypothetical protein | traE |
C5C01_RS28640 | 42332..42526 | - | 195 | Protein_45 | integrase | - |
C5C01_RS24395 | 42640..44000 | - | 1361 | Protein_46 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
C5C01_RS24400 | 44018..44518 | - | 501 | WP_001330556 | prepilin peptidase | - |
C5C01_RS24405 | 44659..45144 | - | 486 | WP_001080767 | lytic transglycosylase domain-containing protein | virB1 |
C5C01_RS24410 | 45189..45725 | - | 537 | WP_000111534 | type 4 pilus major pilin | - |
C5C01_RS24415 | 45787..46881 | - | 1095 | WP_000697158 | type II secretion system F family protein | - |
C5C01_RS24420 | 46883..48391 | - | 1509 | WP_032425678 | ATPase, T2SS/T4P/T4SS family | virB11 |
C5C01_RS24425 | 48495..48953 | - | 459 | WP_000095885 | type IV pilus biogenesis protein PilP | - |
C5C01_RS24430 | 48943..50238 | - | 1296 | WP_000129883 | type 4b pilus protein PilO2 | - |
C5C01_RS24435 | 50259..51878 | - | 1620 | WP_001035565 | PilN family type IVB pilus formation outer membrane protein | - |
C5C01_RS24440 | 51910..52347 | - | 438 | WP_000539952 | type IV pilus biogenesis protein PilM | - |
Host bacterium
ID | 4180 | GenBank | NZ_CP026778 |
Plasmid name | 69-3818|unnamed1 | Incompatibility group | IncB/O/K/Z |
Plasmid size | 115128 bp | Coordinate of oriT [Strand] | 4840..4925 [-] |
Host baterium | Shigella dysenteriae strain 69-3818 |
Cargo genes
Drug resistance gene | tet(B), catA1, ant(3'')-Ia, qacE, sul1 |
Virulence gene | - |
Metal resistance gene | merE, merD, merA, merP, merT, merR |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |