Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103735 |
| Name | oriT_pC25-2  |
| Organism | Enterococcus faecalis strain C25 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP030044 (27260..27309 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pC25-2
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 2596 | GenBank | WP_010717222 |
| Name | t4cp2_DOU31_RS14805_pC25-2 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70128.19 Da Isoelectric Point: 6.1807
>WP_010717222.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 29954..51461
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DOU31_RS14785 (DOU31_14780) | 25139..26836 | - | 1698 | WP_138807355 | relaxase/mobilization nuclease domain-containing protein | - |
| DOU31_RS14790 (DOU31_14785) | 26837..27193 | - | 357 | WP_002362408 | plasmid mobilization relaxosome protein MobC | - |
| DOU31_RS14795 (DOU31_14790) | 27439..27711 | - | 273 | WP_002362409 | LtrD | - |
| DOU31_RS14800 (DOU31_14795) | 27744..29903 | - | 2160 | WP_138807357 | ArdC-like ssDNA-binding domain-containing protein | - |
| DOU31_RS14805 (DOU31_14800) | 29954..31783 | - | 1830 | WP_010717222 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| DOU31_RS14810 (DOU31_14805) | 31783..32268 | - | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| DOU31_RS14815 (DOU31_14810) | 32310..32492 | - | 183 | WP_002367689 | hypothetical protein | - |
| DOU31_RS14820 (DOU31_14815) | 32538..32996 | - | 459 | WP_010717221 | single-stranded DNA-binding protein | - |
| DOU31_RS14825 (DOU31_14820) | 33013..33768 | - | 756 | WP_002415346 | Fic family protein | - |
| DOU31_RS14830 (DOU31_14825) | 33882..34490 | - | 609 | WP_010717220 | hypothetical protein | - |
| DOU31_RS14835 (DOU31_14830) | 34474..34719 | - | 246 | WP_002365866 | hypothetical protein | - |
| DOU31_RS14840 (DOU31_14835) | 34706..35332 | - | 627 | WP_002399631 | hypothetical protein | prgL |
| DOU31_RS14850 (DOU31_14845) | 35605..37539 | - | 1935 | WP_002367922 | group II intron reverse transcriptase/maturase | - |
| DOU31_RS14860 (DOU31_14855) | 38263..40774 | - | 2512 | Protein_42 | phage tail tip lysozyme | - |
| DOU31_RS14865 (DOU31_14860) | 40786..43175 | - | 2390 | Protein_43 | TraC-F-type conjugal transfer protein | - |
| DOU31_RS14870 (DOU31_14865) | 43129..43482 | - | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| DOU31_RS14875 (DOU31_14870) | 43484..44284 | - | 801 | WP_002382799 | type IV secretion system protein | prgHb |
| DOU31_RS14880 (DOU31_14875) | 44299..44595 | - | 297 | WP_002382798 | hypothetical protein | - |
| DOU31_RS14885 (DOU31_14880) | 44608..44835 | - | 228 | WP_002365858 | hypothetical protein | - |
| DOU31_RS14890 (DOU31_14885) | 44882..45316 | - | 435 | WP_002365857 | hypothetical protein | - |
| DOU31_RS14895 (DOU31_14890) | 45336..46235 | - | 900 | WP_002409287 | hypothetical protein | - |
| DOU31_RS14900 (DOU31_14895) | 46277..47134 | - | 858 | WP_126268506 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| DOU31_RS14905 (DOU31_14900) | 47162..47518 | - | 357 | WP_002367672 | pheromone response system RNA-binding regulator PrgU | - |
| DOU31_RS14910 (DOU31_14905) | 47580..51461 | - | 3882 | WP_102990634 | LPXTG-anchored aggregation substance Asa1 | prgB |
| DOU31_RS14915 (DOU31_14910) | 51673..52062 | - | 390 | WP_002425094 | hypothetical protein | - |
| DOU31_RS14920 (DOU31_14915) | 52336..52530 | - | 195 | WP_002425095 | hypothetical protein | - |
| DOU31_RS14930 (DOU31_14925) | 52990..53268 | - | 279 | WP_010717217 | hypothetical protein | - |
| DOU31_RS14935 (DOU31_14930) | 53287..55959 | - | 2673 | WP_138807359 | surface exclusion protein SEA1/PrgA | - |
| DOU31_RS14940 (DOU31_14935) | 55970..56239 | - | 270 | WP_162301544 | hypothetical protein | - |
Host bacterium
| ID | 4175 | GenBank | NZ_CP030044 |
| Plasmid name | pC25-2 | Incompatibility group | - |
| Plasmid size | 87767 bp | Coordinate of oriT [Strand] | 27260..27309 [-] |
| Host baterium | Enterococcus faecalis strain C25 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | asa1 |
| Metal resistance gene | tcrB |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |