Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103726
Name   oriT_pJ2_6 in_silico
Organism   Bacillus cereus strain J2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP022933 (426..466 [-], 41 nt)
oriT length   41 nt
IRs (inverted repeats)      14..22, 29..37  (TAAAGTATA..TATACTTTA)
 2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 41 nt

>oriT_pJ2_6
CACTTTATGGATATAAAGTATAGTGTGTTATACTTTACATG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2760 GenBank   WP_236794100
Name   mobV_L6497_RS29990_pJ2_6 insolico UniProt ID   _
Length   420 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 420 a.a.        Molecular weight: 49690.35 Da        Isoelectric Point: 9.9693

>WP_236794100.1 MobV family relaxase [Bacillus cereus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYTTVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure



No available structure.




Host bacterium

ID   4169 GenBank   NZ_AP022933
Plasmid name   pJ2_6 Incompatibility group   -
Plasmid size   4020 bp Coordinate of oriT [Strand]   426..466 [-]
Host baterium   Bacillus cereus strain J2

Cargo genes

Drug resistance gene   erm(C)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -