Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103724
• Name: oriT_pMRY14-0060_7
• Organism: Bacillus cereus strain MRY14-0060
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022884 (2995..3035 [-], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pMRY14-0060_7
CACTTTATGGATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_L6481_RS29570_pMRY14-0060_7

• ID: 2758
• GenBank: WP_236794100
• Name: mobV_L6481_RS29570_pMRY14-0060_7
• UniProt ID: _
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49690.35 Da
• Isoelectric Point: 9.9693

>WP_236794100.1 MobV family relaxase [Bacillus cereus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYTTVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 4167
• GenBank: NZ_AP022884
• Plasmid name: pMRY14-0060_7
• Incompatibility group: -
• Plasmid size: 4021 bp
• Coordinate of oriT [Strand]: 2995..3035 [-]
• Host baterium: Bacillus cereus strain MRY14-0060

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -