Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103723
• Name: oriT_pMRY14-0074_6
• Organism: Bacillus cereus strain MRY14-0074
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022892 (4445..4466 [-], 22 nt)
• oriT length: 22 nt
• IRs (inverted repeats): 1..8, 15..22 (AAAGTGTA..TACACTTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 22 nt

>oriT_pMRY14-0074_6
AAAGTGTAGTGTGTTACACTTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_L6477_RS28235_pMRY14-0074_6

• ID: 2757
• GenBank: WP_053082646
• Name: mobV_L6477_RS28235_pMRY14-0074_6
• UniProt ID: A0A5A7VNR4
• Length: 448 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 448 a.a.
• Molecular weight: 52610.62 Da
• Isoelectric Point: 9.3407

>WP_053082646.1 MULTISPECIES: MobV family relaxase [Terrabacteria group]
MSYSIIRMQKMKSNGIKGIQFHNQRERESETNPDINKADSHLNYDLINGQRQIDYNEKISQVIEDNVTSG
KKIRKDAVKLAEFLITSDKEFFDKISPKEQKRYFETAHEFLADRYGEKNLIYATVHYDEKTPHMHVGFVP
VTEDGRLSAKDFFGQKKQLVSLQDDFNQYLKQNDFNLERGVSSSRKHVETAKYKAQTFQSMEKEAQEKYE
RTMGHIQEIDDKSKSIANIEAKKVLGLVGMKEQDYKSLVDYATNGVAYQLQAENLQKELEKTQKEVTQLK
ADMQIGQDKIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQLKEKKELKQQV
DTLKIENRSYQNENRELKLENGKLKGKLVQISKEFSVFKERVGKVLHAQLDRVKTFLRMNDVDRSTIKFL
DDRQDKLVQDSLEKMEKPQRQKGMEMER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-195)

Protein structure

• AlphaFold DB: A0A5A7VNR4

Host bacterium

• ID: 4166
• GenBank: NZ_AP022892
• Plasmid name: pMRY14-0074_6
• Incompatibility group: -
• Plasmid size: 4697 bp
• Coordinate of oriT [Strand]: 4445..4466 [-]
• Host baterium: Bacillus cereus strain MRY14-0074

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -