Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103642
• Name: oriT_pDM86-optrA
• Organism: Enterococcus faecalis strain DM86
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP116964 (36872..36912 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pDM86-optrA
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_PO880_RS15035_pDM86-optrA

• ID: 2713
• GenBank: WP_272731721
• Name: mobV_PO880_RS15035_pDM86-optrA
• UniProt ID: _
• Length: 306 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 306 a.a.
• Molecular weight: 36017.83 Da
• Isoelectric Point: 6.1112

>WP_272731721.1 MobV family relaxase [Enterococcus faecalis]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKSTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIARGQKGSERKNLTVPEFKKLKEEELEIKKELQIKKD
ELIAYTKENQIDKKLDITPIKEMEDIEIETDEKTLFGKNKTEIVRQWTGNIILSENDYLKLNKEIKKGKK
TEGRLAAILETDVYQENKELKNELKD

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 4085
• GenBank: NZ_CP116964
• Plasmid name: pDM86-optrA
• Incompatibility group: -
• Plasmid size: 54078 bp
• Coordinate of oriT [Strand]: 36872..36912 [+]
• Host baterium: Enterococcus faecalis strain DM86

Cargo genes

• Drug resistance gene: aac(6')-aph(2''), aph(2'')-Ia, erm(A), optrA, fexA, aadD, ant(9)-Ia
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21