Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103624
Name   oriT_pKP2722-3 in_silico
Organism   Klebsiella pneumoniae strain KP2722
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP116906 (16631..16712 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pKP2722-3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2704 GenBank   WP_032440569
Name   Relaxase_PO783_RS29875_pKP2722-3 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   2534 GenBank   WP_032440593
Name   trbC_PO783_RS30230_pKP2722-3 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 72188..86199

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO783_RS30215 (PO783_30215) 67951..68646 - 696 WP_254910806 RepA family replication protein -
PO783_RS30220 (PO783_30220) 69152..69403 - 252 WP_113394424 hypothetical protein -
PO783_RS30225 (PO783_30225) 69415..69948 - 534 WP_032440594 phospholipase D family protein -
PO783_RS30230 (PO783_30230) 69994..72186 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
PO783_RS30235 (PO783_30235) 72188..73279 - 1092 WP_050597336 thioredoxin fold domain-containing protein trbB
PO783_RS30240 (PO783_30240) 73290..74708 - 1419 WP_063666705 hypothetical protein trbA
PO783_RS30245 (PO783_30245) 74810..75433 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
PO783_RS30250 (PO783_30250) 75499..77643 - 2145 WP_143458320 DotA/TraY family protein traY
PO783_RS30255 (PO783_30255) 77685..78266 - 582 WP_050597335 conjugal transfer protein TraX -
PO783_RS30260 (PO783_30260) 78263..79459 - 1197 WP_032440590 conjugal transfer protein TraW traW
PO783_RS30265 (PO783_30265) 79469..82516 - 3048 WP_032440589 conjugal transfer protein traU
PO783_RS30270 (PO783_30270) 82526..83089 - 564 WP_045325070 hypothetical protein traT
PO783_RS30275 (PO783_30275) 83133..83534 - 402 WP_032440588 DUF6750 family protein traR
PO783_RS30280 (PO783_30280) 83590..84123 - 534 WP_032440587 conjugal transfer protein TraQ traQ
PO783_RS30285 (PO783_30285) 84133..84852 - 720 WP_050597333 conjugal transfer protein TraP traP
PO783_RS30290 (PO783_30290) 84859..86199 - 1341 WP_032440586 conjugal transfer protein TraO traO

Host bacterium

ID   4067 GenBank   NZ_CP116906
Plasmid name   pKP2722-3 Incompatibility group   IncFII
Plasmid size   86599 bp Coordinate of oriT [Strand]   16631..16712 [-]
Host baterium   Klebsiella pneumoniae strain KP2722

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2, tet(A), catA2, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -