Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103600 |
| Name | oriT_36|P1  |
| Organism | Klebsiella oxytoca isolate 36 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW968313 (46656..46761 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_36|P1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2690 | GenBank | WP_004187323 |
| Name | Relaxase_LQ197_RS29195_36|P1 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 2516 | GenBank | WP_004206885 |
| Name | t4cp2_LQ197_RS28845_36|P1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 49557..67058
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ197_RS29175 (AI2991V1_5586) | 44641..44952 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ197_RS29180 (AI2991V1_5587) | 45085..45621 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ197_RS29185 (AI2991V1_5589) | 46123..46518 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ197_RS29190 | 46553..47080 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ197_RS29195 (AI2991V1_5591) | 47067..49046 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ197_RS29200 (AI2991V1_5592) | 49060..49560 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ197_RS29205 (AI2991V1_5593) | 49557..50336 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ197_RS29210 (AI2991V1_5594) | 50347..51510 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ197_RS29215 (AI2991V1_5595) | 51500..51760 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ197_RS29220 (AI2991V1_5596) | 51785..55222 | + | 3438 | WP_225373171 | LPD7 domain-containing protein | - |
| LQ197_RS29225 (AI2991V1_5597) | 55188..55700 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ197_RS29230 (AI2991V1_5598) | 55701..55913 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ197_RS29235 (AI2991V1_5599) | 55885..56295 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ197_RS29240 (AI2991V1_5600) | 56363..57076 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ197_RS29245 | 57085..58236 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ197_RS29250 (AI2991V1_5603) | 58248..59597 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ197_RS29255 (AI2991V1_5604) | 59609..60313 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ197_RS29260 (AI2991V1_5605) | 60337..60867 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ197_RS29265 (AI2991V1_5606) | 60884..61273 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ197_RS29270 (AI2991V1_5607) | 61319..61813 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ197_RS29275 (AI2991V1_5608) | 61810..64860 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ197_RS29280 (AI2991V1_5609) | 64980..66065 | + | 1086 | WP_228719183 | conjugal transfer protein TraW | traW |
| LQ197_RS29285 (AI2991V1_5610) | 66062..66658 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ197_RS29290 | 66651..67058 | + | 408 | WP_230133356 | hypothetical protein | traY |
| LQ197_RS29295 (AI2991V1_REPA000000101) | 67092..68303 | - | 1212 | Protein_92 | IS66 family transposase | - |
| LQ197_RS29300 (AI2991V1_5612) | 68336..71320 | - | 2985 | WP_004207017 | Tn3 family transposase | - |
| LQ197_RS29305 | 71375..71998 | - | 624 | WP_004207019 | recombinase family protein | - |
Host bacterium
| ID | 4043 | GenBank | NZ_OW968313 |
| Plasmid name | 36|P1 | Incompatibility group | IncL/M |
| Plasmid size | 80364 bp | Coordinate of oriT [Strand] | 46656..46761 [+] |
| Host baterium | Klebsiella oxytoca isolate 36 |
Cargo genes
| Drug resistance gene | blaVIM-1, dfrB1, ant(3'')-Ia, catB2, qacE, sul1, mph(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |