Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103590
Name   oriT_101|P2 in_silico
Organism   Klebsiella pneumoniae isolate 101
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW970388 (76337..76386 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_101|P2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2684 GenBank   WP_015065542
Name   traI_LQV33_RS27460_101|P2 insolico UniProt ID   K7R3U7
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190318.03 Da        Isoelectric Point: 6.2890

>WP_015065542.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMSREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALAVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAAGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDTAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGGGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA helicase TraI (TraI_C)

(1432-1554)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52874..76944

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV33_RS27465 (AI3008V1_5168) 47976..50289 - 2314 Protein_49 type IV conjugative transfer system coupling protein TraD -
LQV33_RS27470 (AI3008V1_5170) 50418..51107 - 690 WP_015065540 hypothetical protein -
LQV33_RS27475 (AI3008V1_5171) 51299..52030 - 732 WP_032329926 conjugal transfer complement resistance protein TraT -
LQV33_RS27480 (AI3008V1_5172) 52339..52863 - 525 WP_015065538 hypothetical protein -
LQV33_RS27485 (AI3008V1_5173) 52874..55717 - 2844 WP_015065537 conjugal transfer mating-pair stabilization protein TraG traG
LQV33_RS27490 (AI3008V1_5174) 55717..57087 - 1371 WP_142499963 conjugal transfer pilus assembly protein TraH traH
LQV33_RS27495 (AI3008V1_5175) 57074..57502 - 429 WP_015065560 hypothetical protein -
LQV33_RS27500 (AI3008V1_5176) 57495..58067 - 573 WP_023292147 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
LQV33_RS27505 (AI3008V1_5177) 58039..58278 - 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
LQV33_RS27510 (AI3008V1_5178) 58289..59041 - 753 WP_020803149 type-F conjugative transfer system pilin assembly protein TraF traF
LQV33_RS27515 (AI3008V1_5179) 59062..59388 - 327 WP_004144402 hypothetical protein -
LQV33_RS27520 (AI3008V1_5180) 59401..59649 - 249 WP_004152675 hypothetical protein -
LQV33_RS27525 (AI3008V1_5181) 59627..59881 - 255 WP_004152674 conjugal transfer protein TrbE -
LQV33_RS27530 (AI3008V1_5182) 59913..61868 - 1956 WP_229998267 type-F conjugative transfer system mating-pair stabilization protein TraN traN
LQV33_RS27535 (AI3008V1_5183) 61927..62565 - 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
LQV33_RS27540 (AI3008V1_5184) 62578..63567 - 990 WP_011977785 conjugal transfer pilus assembly protein TraU traU
LQV33_RS27545 (AI3008V1_5185) 63564..63953 - 390 WP_015065532 hypothetical protein -
LQV33_RS27550 (AI3008V1_5186) 63995..64621 - 627 WP_009309871 type-F conjugative transfer system protein TraW traW
LQV33_RS27555 (AI3008V1_5187) 64621..65010 - 390 WP_004152506 type-F conjugative transfer system protein TrbI -
LQV33_RS27560 (AI3008V1_5188) 65010..67649 - 2640 WP_004152505 type IV secretion system protein TraC virb4
LQV33_RS27565 (AI3008V1_5189) 67721..68119 - 399 WP_011977783 hypothetical protein -
LQV33_RS27570 68127..68369 - 243 WP_004158946 hypothetical protein -
LQV33_RS27575 (AI3008V1_5190) 68561..68965 - 405 WP_004152503 hypothetical protein -
LQV33_RS27580 (AI3008V1_5191) 69032..69343 - 312 WP_004152502 hypothetical protein -
LQV33_RS27585 (AI3008V1_5192) 69344..69562 - 219 WP_004152501 hypothetical protein -
LQV33_RS27590 (AI3008V1_5193) 69668..70078 - 411 WP_032329942 hypothetical protein -
LQV33_RS27595 (AI3008V1_5194) 70210..70794 - 585 WP_015065529 type IV conjugative transfer system lipoprotein TraV traV
LQV33_RS27600 (AI3008V1_5195) 70908..72332 - 1425 WP_004194260 F-type conjugal transfer pilus assembly protein TraB traB
LQV33_RS27605 (AI3008V1_5196) 72332..73072 - 741 WP_015065528 type-F conjugative transfer system secretin TraK traK
LQV33_RS27610 (AI3008V1_5197) 73059..73625 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
LQV33_RS27615 (AI3008V1_5198) 73645..73950 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
LQV33_RS27620 (AI3008V1_5199) 73964..74332 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
LQV33_RS27625 74397..74600 - 204 WP_171486139 TraY domain-containing protein -
LQV33_RS27630 (AI3008V1_5200) 74733..75452 - 720 WP_015065526 hypothetical protein -
LQV33_RS27635 (AI3008V1_5201) 75652..76068 - 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
LQV33_RS27640 (AI3008V1_5202) 76459..76944 + 486 WP_015065525 transglycosylase SLT domain-containing protein virB1
LQV33_RS27645 76977..77306 - 330 WP_015065524 DUF5983 family protein -
LQV33_RS27650 (AI3008V1_5203) 77339..78161 - 823 Protein_86 DUF932 domain-containing protein -
LQV33_RS27655 (AI3008V1_5204) 78985..79548 - 564 WP_004152756 methyltransferase -
LQV33_RS27660 (AI3008V1_5205) 79596..80951 - 1356 WP_015065512 DUF3560 domain-containing protein -
LQV33_RS27665 (AI3008V1_5206) 81003..81233 - 231 WP_009310031 hypothetical protein -
LQV33_RS27670 (AI3008V1_5207) 81325..81552 - 228 WP_001568050 hypothetical protein -

Host bacterium

ID   4033 GenBank   NZ_OW970388
Plasmid name   101|P2 Incompatibility group   IncFII
Plasmid size   91795 bp Coordinate of oriT [Strand]   76337..76386 [+]
Host baterium   Klebsiella pneumoniae isolate 101

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -