Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 103583
• Name: oriT_8|P3
• Organism: Citrobacter freundii isolate 8
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_OW849085 (28857..28957 [+], 101 nt)
• oriT length: 101 nt
• IRs (inverted repeats): 80..85, 91..96 (AAAAAA..TTTTTT) 20..26, 38..44 (TAAATCA..TGATTTA)
• Location of nic site: 62..63
• Conserved sequence flanking the nic site: GGTGTATAGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 101 nt

>oriT_8|P3
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

TrwC_LQ154_RS26885_8|P3

• ID: 2680
• GenBank: WP_230129924
• Name: TrwC_LQ154_RS26885_8|P3
• UniProt ID: _
• Length: 594 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 594 a.a.
• Molecular weight: 66164.03 Da
• Isoelectric Point: 9.8552

>WP_230129924.1 MobF family relaxase [Citrobacter freundii]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEGSIEKTGNKAR

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (477-583)

• TrwC relaxase (TrwC) (16-287)

Protein structure

No available structure.

Auxiliary protein

WP_000706094_8|P3

• ID: 1127
• GenBank: WP_000706094
• Name: WP_000706094_8|P3
• UniProt ID: B6UZ32
• Length: 96 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 96 a.a.
• Molecular weight: 10829.54 Da
• Isoelectric Point: 8.5040

>WP_000706094.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MKIVADRLSDVNRKLDYLFDRASDADFGPLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIA
EPGKTKAAKAEVERNGYKVWEPKKER

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: B6UZ32

T4CP

t4cp2_LQ154_RS26890_8|P3

• ID: 2503
• GenBank: WP_001749976
• Name: t4cp2_LQ154_RS26890_8|P3
• UniProt ID: _
• Length: 509 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 509 a.a.
• Molecular weight: 57679.73 Da
• Isoelectric Point: 9.5788

>WP_001749976.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Gammaproteobacteria]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVSARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELRDI

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (113-489)

Protein structure

No available structure.

Host bacterium

• ID: 4026
• GenBank: NZ_OW849085
• Plasmid name: 8|P3
• Incompatibility group: IncN
• Plasmid size: 37891 bp
• Coordinate of oriT [Strand]: 28857..28957 [+]
• Host baterium: Citrobacter freundii isolate 8

Cargo genes

• Drug resistance gene: blaIMP-22, blaOXA-2, qacE, sul1, qnrB2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -