Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103558
Name   oriT_pHD2579-NDM in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain HD2579
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON209137 (50223..50840 [+], 618 nt)
oriT length   618 nt
IRs (inverted repeats)      589..594, 608..613  (TCCATG..CATGGA)
 592..597, 602..607  (ATGCCC..GGGCAT)
 520..527, 530..537  (CCTCCCGT..ACGGGAGG)
 455..460, 464..469  (ATTCGC..GCGAAT)
 197..202, 208..213  (TTCAAT..ATTGAA)
 85..90, 96..101  (TGCTTT..AAAGCA)
Location of nic site      315..316
Conserved sequence flanking the
  nic site    		  TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 618 nt

>oriT_pHD2579-NDM
TTCTTTGTTTCCCTGTCAAATACATTGTGGTTTTGATAAAATCATCATTGTCTTTTGACACATTAACATCTTCATTTTTAACCTTGCTTTTCAGTAAAGCATTGGCAAGCAGCTTCATTCCTTTTGCCACGGTTAAACCCGGTTCATCTGGATAAAGTGTTTTAAGACAATCAAGTAGATCATCATGTTGGCTTTCTTCAATCCTGAATTGAACTTTTTTCATAAAACTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAACTACCTCCACACATAAATTGTGCTTCAGCGTCCATCGAAATGCAAAAAATGAAAGGCTATCGTGGCTGTGATCTGGTGCGTCTGCGACCGGAGACAACGGATTCGCGGCTAACTTGGGCGGAAATAAATTCGCTACGCGAATGGCCTGGCAGGGGGCGCGAGCGCTGTTTTACGGAATATACAAAAAAAGCACCTCCCGTAAACGGGAGGGCTTCGGCGATTCAGGAACGGGAATTTTATTCTGCCTCTGGTGTGTCGCCTTCCATGCCCTGCCGGGCATCATGGAGCCAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2662 GenBank   WP_004199101
Name   Relaxase_OZD89_RS00300_pHD2579-NDM insolico UniProt ID   A0A515HL38
Length   386 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 386 a.a.        Molecular weight: 44388.82 Da        Isoelectric Point: 10.6017

>WP_004199101.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMIDRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMNKKYPNHRFVLGYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYD
VKATHKQQHGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYWGADF
GELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGVKGIDRISSSSKELVLN
SAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(90-240)


  Protein structure


Source ID Structure
AlphaFold DB A0A515HL38


T4CP

ID   2480 GenBank   WP_015060057
Name   t4cp2_OZD89_RS00275_pHD2579-NDM insolico UniProt ID   _
Length   917 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 917 a.a.        Molecular weight: 104788.72 Da        Isoelectric Point: 6.2666

>WP_015060057.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYALILPGWYAIKRVTKIDERFFDLLYLR
MQIKGNPLANKRFNAVHYAGSSYDAVDISKVDNFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQ
IDGAYFECVDEADLALLTDQLNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSA
AEYFENKLYLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLYEENN
IVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARYFRCIEIKDYFQETDAG
IFDALMYLPVEYVQTSSLTPIDKQSAIKALDDQIDKLEMTDDAAKSLLADLKVGLDMVSSGYISFGKSHQ
TLIVYADSPERLVKDTNIVTTTLEDLGLIVTYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFH
NFFTGKEKGNTWGNNLITLGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFA
AQQFGTPESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNISFMKDL
VRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTETRRHGLKARLNAWVQGGEF
GWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYLIYRVTMLADGRRLLIYMDEFWQWINNEAFR
DFVYNKLKTARKLDMVLVVATQSPDELIKSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKI
KAIDPLSRQFLVVKNPQRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTY
LERANLI

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(272-474)

Type IV secretory pathway, VirB3-like protein (VirB3)

(2-83)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 37533..47642

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OZD89_RS00205 32768..33262 - 495 WP_001215543 thermonuclease family protein -
OZD89_RS00210 33259..33585 - 327 WP_004208538 hypothetical protein -
OZD89_RS00215 33671..33985 - 315 WP_000754496 TrbM/KikA/MpfK family conjugal transfer protein -
OZD89_RS00220 34073..34465 - 393 WP_000738524 cag pathogenicity island Cag12 family protein -
OZD89_RS00230 35824..37530 - 1707 Protein_46 type IV secretory system conjugative DNA transfer family protein -
OZD89_RS00235 37533..38567 - 1035 WP_000650512 P-type DNA transfer ATPase VirB11 virB11
OZD89_RS00240 38564..38761 - 198 WP_000629109 DNA-binding protein -
OZD89_RS00245 38763..39977 - 1215 WP_001295060 VirB10/TraB/TrbI family type IV secretion system protein virB10
OZD89_RS00250 39974..40903 - 930 WP_000783386 TrbG/VirB9 family P-type conjugative transfer protein virB9
OZD89_RS00255 40909..41637 - 729 WP_004199085 type IV secretion system protein virB8
OZD89_RS00260 41832..42887 - 1056 WP_000235886 type IV secretion system protein virB6
OZD89_RS00265 42899..43156 - 258 WP_000759841 EexN family lipoprotein -
OZD89_RS00270 43166..43936 - 771 WP_000716326 type IV secretion system protein -
OZD89_RS00275 43946..46699 - 2754 WP_015060057 VirB3 family type IV secretion system protein virb4
OZD89_RS00280 46724..47014 - 291 WP_000916156 TrbC/VirB2 family protein virB2
OZD89_RS00285 46998..47642 - 645 WP_000953538 lytic transglycosylase domain-containing protein virB1
OZD89_RS00290 47688..47924 - 237 WP_001348696 hypothetical protein -
OZD89_RS00295 47875..48525 - 651 WP_004208545 transcription termination/antitermination NusG family protein -
OZD89_RS00300 48737..49897 - 1161 WP_004199101 MobP1 family relaxase -
OZD89_RS00305 49900..50445 - 546 WP_001348698 DNA distortion polypeptide 1 -
OZD89_RS00310 50786..51043 - 258 WP_004199355 hypothetical protein -
OZD89_RS00315 51040..51192 - 153 WP_162542717 hypothetical protein -
OZD89_RS00320 51279..51617 - 339 WP_001099725 hypothetical protein -
OZD89_RS00325 51711..51953 - 243 WP_000008707 hypothetical protein -
OZD89_RS00330 51943..52224 - 282 WP_001348701 hypothetical protein -

Host bacterium

ID   4001 GenBank   NZ_ON209137
Plasmid name   pHD2579-NDM Incompatibility group   IncX3
Plasmid size   54994 bp Coordinate of oriT [Strand]   50223..50840 [+]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain HD2579

Cargo genes

Drug resistance gene   blaSHV-12, blaNDM-1
Virulence gene   htpB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -