Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103551
Name   oriT_147|P1 in_silico
Organism   Klebsiella pneumoniae isolate 147
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW970380 (104605..104686 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_147|P1
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2657 GenBank   WP_015632446
Name   Relaxase_LQU99_RS26295_147|P1 insolico UniProt ID   R4WBX2
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74631.63 Da        Isoelectric Point: 9.3060

>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB R4WBX2


T4CP

ID   2474 GenBank   WP_015632442
Name   trbC_LQU99_RS26260_147|P1 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82688.74 Da        Isoelectric Point: 5.8919

>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(442-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74903..96744

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQU99_RS26125 (AI2856V1_4940) 70456..70842 - 387 WP_015632417 acyl-CoA thioesterase -
LQU99_RS26130 (AI2856V1_4941) 70842..71312 - 471 WP_032441927 hypothetical protein -
LQU99_RS26135 (AI2856V1_4942) 71294..71872 - 579 WP_015632419 hypothetical protein -
LQU99_RS26140 (AI2856V1_4943) 72140..72673 + 534 WP_015632420 transcription termination/antitermination NusG family protein -
LQU99_RS26145 (AI2856V1_4944) 72691..72894 + 204 WP_032495743 hypothetical protein -
LQU99_RS26150 (AI2856V1_4945) 72928..73239 + 312 WP_015632421 hypothetical protein -
LQU99_RS26155 (AI2856V1_4947) 73937..74416 + 480 WP_015632423 lytic transglycosylase domain-containing protein -
LQU99_RS26160 (AI2856V1_4948) 74439..74906 + 468 WP_015632424 DotD/TraH family lipoprotein -
LQU99_RS26165 (AI2856V1_4949) 74903..75721 + 819 WP_015632425 type IV secretory system conjugative DNA transfer family protein traI
LQU99_RS26170 (AI2856V1_4950) 75699..76886 + 1188 WP_032495744 plasmid transfer ATPase TraJ virB11
LQU99_RS26175 (AI2856V1_4951) 76883..77149 + 267 WP_032441926 IcmT/TraK family protein traK
LQU99_RS26180 (AI2856V1_4952) 77167..80490 + 3324 WP_032495745 LPD7 domain-containing protein -
LQU99_RS26185 (AI2856V1_4953) 80524..80877 + 354 WP_015632427 hypothetical protein traL
LQU99_RS26190 (AI2856V1_4954) 80913..81617 + 705 WP_015632428 DotI/IcmL/TraM family protein traM
LQU99_RS26195 (AI2856V1_4955) 81628..82731 + 1104 WP_015632429 DotH/IcmK family type IV secretion protein traN
LQU99_RS26200 (AI2856V1_4956) 82735..84075 + 1341 WP_015632430 conjugal transfer protein TraO traO
LQU99_RS26205 (AI2856V1_4957) 84081..84800 + 720 WP_015632431 conjugal transfer protein TraP traP
LQU99_RS26210 (AI2856V1_4958) 84810..85343 + 534 WP_015632432 conjugal transfer protein TraQ traQ
LQU99_RS26215 (AI2856V1_4959) 85397..85798 + 402 WP_015632433 DUF6750 family protein traR
LQU99_RS26220 (AI2856V1_4960) 85842..86405 + 564 WP_032495746 hypothetical protein traT
LQU99_RS26225 (AI2856V1_4961) 86415..89462 + 3048 WP_015632435 conjugal transfer protein traU
LQU99_RS26230 (AI2856V1_4962) 89473..90669 + 1197 WP_015632436 conjugal transfer protein TraW traW
LQU99_RS26235 (AI2856V1_4963) 90666..91247 + 582 WP_015632437 conjugal transfer protein TraX -
LQU99_RS26240 (AI2856V1_4964) 91289..93433 + 2145 WP_015632438 DotA/TraY family protein traY
LQU99_RS26245 (AI2856V1_4965) 93499..94122 + 624 WP_015632439 plasmid IncI1-type surface exclusion protein ExcA -
LQU99_RS26250 (AI2856V1_4966) 94224..95642 + 1419 WP_015632440 hypothetical protein trbA
LQU99_RS26255 (AI2856V1_4967) 95617..96744 + 1128 WP_157787237 thioredoxin fold domain-containing protein trbB
LQU99_RS26260 (AI2856V1_4968) 96746..98938 + 2193 WP_015632442 F-type conjugative transfer protein TrbC -
LQU99_RS26265 (AI2856V1_4969) 98984..99289 + 306 WP_015632443 phospholipase D-like domain-containing protein -
LQU99_RS26270 (AI2856V1_4970) 99312..100901 - 1590 WP_015632444 IS66 family transposase -
LQU99_RS26275 (AI2856V1_4971) 100931..101280 - 350 Protein_101 IS66 family insertion sequence element accessory protein TnpB -
LQU99_RS26280 101277..101684 - 408 WP_015632445 transposase -

Host bacterium

ID   3994 GenBank   NZ_OW970380
Plasmid name   147|P1 Incompatibility group   IncFII
Plasmid size   121082 bp Coordinate of oriT [Strand]   104605..104686 [-]
Host baterium   Klebsiella pneumoniae isolate 147

Cargo genes

Drug resistance gene   rmtF, ARR-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -