Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103545 |
| Name | oriT_395|P1  |
| Organism | Klebsiella pneumoniae isolate 395 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW970501 (96087..96192 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_395|P1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2653 | GenBank | WP_004187323 |
| Name | Relaxase_LQV51_RS28450_395|P1 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 2468 | GenBank | WP_004206885 |
| Name | t4cp2_LQV51_RS27800_395|P1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 98988..118277
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQV51_RS28430 (AI2934V1_5271) | 94072..94383 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQV51_RS28435 (AI2934V1_5272) | 94516..95052 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQV51_RS28440 (AI2934V1_5273) | 95554..95949 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQV51_RS28445 (AI2934V1_5274) | 95984..96511 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQV51_RS28450 (AI2934V1_5275) | 96498..98477 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQV51_RS28455 (AI2934V1_5276) | 98491..98991 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQV51_RS28460 (AI2934V1_5277) | 98988..99767 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQV51_RS28465 (AI2934V1_5278) | 99778..100941 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQV51_RS28470 (AI2934V1_5279) | 100931..101191 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQV51_RS28475 (AI2934V1_5280) | 101216..104653 | + | 3438 | WP_225373171 | LPD7 domain-containing protein | - |
| LQV51_RS28480 (AI2934V1_5281) | 104619..105131 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQV51_RS28485 | 105132..105344 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQV51_RS28490 (AI2934V1_5282) | 105316..105726 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQV51_RS28495 (AI2934V1_5283) | 105794..106507 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQV51_RS28500 (AI2934V1_5284) | 106516..107667 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQV51_RS28505 (AI2934V1_5285) | 107679..109028 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQV51_RS28510 (AI2934V1_5286) | 109040..109744 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQV51_RS28515 (AI2934V1_5287) | 109768..110298 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQV51_RS28520 (AI2934V1_5288) | 110315..110704 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQV51_RS28525 (AI2934V1_5289) | 110750..111244 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQV51_RS28530 (AI2934V1_5290) | 111241..114291 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQV51_RS28535 (AI2934V1_5291) | 114288..115496 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LQV51_RS28540 (AI2934V1_5292) | 115493..116089 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQV51_RS28545 (AI2934V1_5293) | 116082..118277 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQV51_RS28550 (AI2934V1_5294) | 118279..118932 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQV51_RS28555 (AI2934V1_5295) | 119013..119243 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 3988 | GenBank | NZ_OW970501 |
| Plasmid name | 395|P1 | Incompatibility group | IncR |
| Plasmid size | 119538 bp | Coordinate of oriT [Strand] | 96087..96192 [+] |
| Host baterium | Klebsiella pneumoniae isolate 395 |
Cargo genes
| Drug resistance gene | tet(A), dfrA1, qacE, sul1, aac(6')-Ib-cr, blaOXA-1, blaCTX-M-15, catA1, blaTEM-1C |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |