Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103506 |
| Name | oriT_pkh2101  |
| Organism | Lactococcus garvieae strain KH2101 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_ON321837 (24984..25013 [+], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_pkh2101
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2627 | GenBank | WP_252501464 |
| Name | MobA_MobL_NIS44_RS00135_pkh2101 |
UniProt ID | _ |
| Length | 654 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 654 a.a. Molecular weight: 76571.55 Da Isoelectric Point: 9.3532
>WP_252501464.1 MobA/MobL family protein [Lactococcus garvieae]
MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDRQTLWNKMELA
EKSPNAQLCRELNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLTMREVDSE
GNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNIDQQITEK
SHAELGKKELPKIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQNFTLKFEK
TFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANE
ILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKKIFKEKPF
QTSRYLDSKIKQVEDSITKEKNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEML
LAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHIDELSPLAK
QNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSGNQLQKKR
NLDKLIKQTKAKENQSLQRNIPLR
MTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTIKPEAFILKPDYVPDEFLDRQTLWNKMELA
EKSPNAQLCRELNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLTMREVDSE
GNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNIDQQITEK
SHAELGKKELPKIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQNFTLKFEK
TFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANE
ILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKKIFKEKPF
QTSRYLDSKIKQVEDSITKEKNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEML
LAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHIDELSPLAK
QNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSGNQLQKKR
NLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2434 | GenBank | WP_032495476 |
| Name | t4cp2_NIS44_RS00090_pkh2101 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63052.07 Da Isoelectric Point: 4.7040
>WP_032495476.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactobacillales]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKETIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKETIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10231..22518
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NIS44_RS00035 | 5418..5588 | - | 171 | WP_000713595 | hypothetical protein | - |
| NIS44_RS00040 | 5936..7426 | - | 1491 | WP_044980265 | primase C-terminal domain-containing protein | - |
| NIS44_RS00045 | 7469..7768 | - | 300 | WP_002325779 | hypothetical protein | - |
| NIS44_RS00050 | 8147..8395 | - | 249 | WP_010752677 | helix-turn-helix transcriptional regulator | - |
| NIS44_RS00055 | 8865..9602 | - | 738 | WP_002292226 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| NIS44_RS00060 | 9727..9810 | - | 84 | WP_032501549 | 23S rRNA methyltransferase attenuator leader peptide ErmL | - |
| NIS44_RS00160 | 9859..9942 | - | 84 | Protein_12 | MLS leader peptide | - |
| NIS44_RS00065 | 10231..11052 | - | 822 | WP_201688346 | LPXTG cell wall anchor domain-containing protein | prgC |
| NIS44_RS00070 | 11112..11480 | - | 369 | WP_000516429 | hypothetical protein | - |
| NIS44_RS00075 | 11509..12480 | - | 972 | WP_002425009 | hypothetical protein | - |
| NIS44_RS00080 | 12497..13429 | - | 933 | WP_002325632 | conjugal transfer protein TrbL family protein | trsL |
| NIS44_RS00085 | 13431..14354 | - | 924 | WP_252501463 | hypothetical protein | - |
| NIS44_RS00090 | 14372..16027 | - | 1656 | WP_032495476 | VirD4-like conjugal transfer protein, CD1115 family | - |
| NIS44_RS00095 | 16020..16451 | - | 432 | WP_032495475 | hypothetical protein | - |
| NIS44_RS00100 | 16456..17007 | - | 552 | WP_000393725 | hypothetical protein | - |
| NIS44_RS00105 | 17020..18129 | - | 1110 | WP_000501303 | lysozyme family protein | orf14 |
| NIS44_RS00110 | 18151..19503 | - | 1353 | WP_032495474 | hypothetical protein | trsF |
| NIS44_RS00115 | 19517..21478 | - | 1962 | WP_032495473 | TrsE | virb4 |
| NIS44_RS00120 | 21489..22118 | - | 630 | WP_002325627 | hypothetical protein | trsD |
| NIS44_RS00125 | 22135..22518 | - | 384 | WP_001083514 | glycosyl transferase | trsC |
| NIS44_RS00130 | 22537..22869 | - | 333 | WP_032495472 | CagC family type IV secretion system protein | - |
| NIS44_RS00135 | 22893..24878 | - | 1986 | WP_336251314 | MobQ family relaxase | - |
| NIS44_RS00140 | 25170..25469 | + | 300 | WP_002325624 | hypothetical protein | - |
| NIS44_RS00145 | 25472..25729 | + | 258 | WP_000002668 | hypothetical protein | - |
| NIS44_RS00150 | 25752..26057 | - | 306 | WP_252501465 | hypothetical protein | - |
| NIS44_RS00155 | 26091..26588 | - | 498 | WP_002300565 | DnaJ domain-containing protein | - |
Host bacterium
| ID | 3949 | GenBank | NZ_ON321837 |
| Plasmid name | pkh2101 | Incompatibility group | - |
| Plasmid size | 26850 bp | Coordinate of oriT [Strand] | 24984..25013 [+] |
| Host baterium | Lactococcus garvieae strain KH2101 |
Cargo genes
| Drug resistance gene | erm(B) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |