Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103500
Name   oriT_AGR_158|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_158
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039141 (22394..22476 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_158|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2621 GenBank   WP_010895859
Name   Relaxase_MOO67_RS00135_AGR_158|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2429 GenBank   WP_241389808
Name   trbC_MOO67_RS00150_AGR_158|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81493.37 Da        Isoelectric Point: 8.5425

>WP_241389808.1 F-type conjugative transfer protein TrbC [Serratia entomophila]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVEPPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2430 GenBank   WP_010895767
Name   t4cp2_MOO67_RS00345_AGR_158|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28069..56360

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO67_RS00140 (158p1_00008) 24844..25167 - 324 WP_010895860 hypothetical protein -
MOO67_RS00145 (158p1_00009) 25177..25713 - 537 WP_234591459 phospholipase D family protein -
MOO67_RS00150 (158p1_00010) 25854..28031 - 2178 WP_241389808 F-type conjugative transfer protein TrbC -
MOO67_RS00155 (158p1_00011) 28069..29067 - 999 WP_010895863 hypothetical protein trbB
MOO67_RS00160 (158p1_00012) 29080..30390 - 1311 WP_241389809 conjugal transfer protein TrbA trbA
MOO67_RS00165 (158p1_00013) 30528..31028 - 501 WP_241389810 hypothetical protein -
MOO67_RS00170 (158p1_00014) 31106..33400 - 2295 WP_241389811 DotA/TraY family protein traY
MOO67_RS00175 (158p1_00015) 33315..33878 - 564 WP_010895867 conjugal transfer protein TraX -
MOO67_RS00180 (158p1_00016) 33871..35097 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO67_RS00185 (158p1_00018) 35238..38312 - 3075 WP_241389812 type IV secretory system conjugative DNA transfer family protein traU
MOO67_RS00190 (158p1_00019) 38315..38920 - 606 WP_010895870 TraT traT
MOO67_RS00195 (158p1_00020) 39006..39407 - 402 WP_010895871 DUF6750 family protein traR
MOO67_RS00200 (158p1_00021) 39423..39953 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO67_RS00205 (158p1_00022) 39962..40705 - 744 WP_241389813 hypothetical protein traP
MOO67_RS00210 (158p1_00023) 40705..41937 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO67_RS00215 (158p1_00024) 41942..42949 - 1008 WP_241389814 DotH/IcmK family type IV secretion protein traN
MOO67_RS00220 (158p1_00025) 42952..43626 - 675 WP_241389815 DotI/IcmL/TraM family protein traM
MOO67_RS00225 (158p1_00026) 43717..44208 - 492 WP_010895743 hypothetical protein traL
MOO67_RS00230 (158p1_00027) 44218..47454 - 3237 WP_241389816 LPD7 domain-containing protein -
MOO67_RS00235 (158p1_00029) 47597..47863 - 267 WP_010895745 IcmT/TraK family protein traK
MOO67_RS00240 (158p1_00030) 47863..49020 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOO67_RS00245 (158p1_00032) 49210..49998 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO67_RS00250 (158p1_00033) 49995..50453 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO67_RS00255 (158p1_00034) 50491..51924 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO67_RS00260 (158p1_00035) 51921..52589 - 669 WP_241389817 prepilin peptidase -
MOO67_RS00265 (158p1_00036) 52589..53074 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO67_RS00270 (158p1_00037) 53129..53722 - 594 WP_010895752 type 4 pilus major pilin -
MOO67_RS00275 (158p1_00038) 53758..54870 - 1113 WP_241389818 type II secretion system F family protein -
MOO67_RS00280 (158p1_00039) 54870..56360 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO67_RS00285 (158p1_00040) 56366..56950 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO67_RS00290 (158p1_00041) 56937..58247 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO67_RS00295 (158p1_00042) 58251..59885 - 1635 WP_010895757 PilN family type IVB pilus formation outer membrane protein -
MOO67_RS00300 (158p1_00043) 59910..60335 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO67_RS00305 (158p1_00044) 60335..61348 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3943 GenBank   NZ_MT039141
Plasmid name   AGR_158|unnamed1 Incompatibility group   -
Plasmid size   149883 bp Coordinate of oriT [Strand]   22394..22476 [+]
Host baterium   Serratia entomophila strain AGR_158

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -