Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103499
Name   oriT_AGR_Moraki_2|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_Moraki_2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039162 (22259..22341 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_Moraki_2|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2620 GenBank   WP_010895859
Name   Relaxase_MOO93_RS00135_AGR_Moraki_2|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2427 GenBank   WP_234591458
Name   trbC_MOO93_RS00150_AGR_Moraki_2|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2428 GenBank   WP_010895767
Name   t4cp2_MOO93_RS00345_AGR_Moraki_2|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27934..56225

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO93_RS00140 (Moraki_2p1_00037) 24709..25032 - 324 WP_010895860 hypothetical protein -
MOO93_RS00145 (Moraki_2p1_00038) 25042..25578 - 537 WP_234591459 phospholipase D family protein -
MOO93_RS00150 (Moraki_2p1_00039) 25719..27896 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOO93_RS00155 (Moraki_2p1_00040) 27934..28932 - 999 WP_010895863 hypothetical protein trbB
MOO93_RS00160 (Moraki_2p1_00041) 28945..30255 - 1311 WP_241389809 conjugal transfer protein TrbA trbA
MOO93_RS00165 (Moraki_2p1_00042) 30393..30893 - 501 WP_241389810 hypothetical protein -
MOO93_RS00170 (Moraki_2p1_00043) 30971..33265 - 2295 WP_241389811 DotA/TraY family protein traY
MOO93_RS00175 (Moraki_2p1_00044) 33180..33743 - 564 WP_010895867 conjugal transfer protein TraX -
MOO93_RS00180 (Moraki_2p1_00045) 33736..34962 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO93_RS00185 (Moraki_2p1_00047) 35103..38177 - 3075 WP_241389812 type IV secretory system conjugative DNA transfer family protein traU
MOO93_RS00190 (Moraki_2p1_00048) 38180..38785 - 606 WP_010895870 TraT traT
MOO93_RS00195 (Moraki_2p1_00049) 38871..39272 - 402 WP_010895871 DUF6750 family protein traR
MOO93_RS00200 (Moraki_2p1_00050) 39288..39818 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO93_RS00205 (Moraki_2p1_00051) 39827..40570 - 744 WP_241389813 hypothetical protein traP
MOO93_RS00210 (Moraki_2p1_00052) 40570..41802 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO93_RS00215 (Moraki_2p1_00053) 41807..42814 - 1008 WP_241389814 DotH/IcmK family type IV secretion protein traN
MOO93_RS00220 (Moraki_2p1_00054) 42817..43491 - 675 WP_241389815 DotI/IcmL/TraM family protein traM
MOO93_RS00225 (Moraki_2p1_00055) 43582..44073 - 492 WP_010895743 hypothetical protein traL
MOO93_RS00230 (Moraki_2p1_00056) 44083..47319 - 3237 WP_010895744 LPD7 domain-containing protein -
MOO93_RS00235 (Moraki_2p1_00058) 47462..47728 - 267 WP_010895745 IcmT/TraK family protein traK
MOO93_RS00240 (Moraki_2p1_00059) 47728..48885 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOO93_RS00245 (Moraki_2p1_00061) 49075..49863 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO93_RS00250 (Moraki_2p1_00062) 49860..50318 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO93_RS00255 (Moraki_2p1_00063) 50356..51789 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO93_RS00260 (Moraki_2p1_00064) 51786..52454 - 669 WP_241389817 prepilin peptidase -
MOO93_RS00265 (Moraki_2p1_00065) 52454..52939 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO93_RS00270 (Moraki_2p1_00066) 52994..53587 - 594 WP_010895752 type 4 pilus major pilin -
MOO93_RS00275 (Moraki_2p1_00067) 53623..54735 - 1113 WP_241389818 type II secretion system F family protein -
MOO93_RS00280 (Moraki_2p1_00068) 54735..56225 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO93_RS00285 (Moraki_2p1_00069) 56231..56815 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO93_RS00290 (Moraki_2p1_00070) 56802..58112 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO93_RS00295 (Moraki_2p1_00071) 58116..59750 - 1635 WP_241390125 PilN family type IVB pilus formation outer membrane protein -
MOO93_RS00300 (Moraki_2p1_00072) 59775..60200 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO93_RS00305 (Moraki_2p1_00073) 60200..61213 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3942 GenBank   NZ_MT039162
Plasmid name   AGR_Moraki_2|unnamed1 Incompatibility group   -
Plasmid size   151371 bp Coordinate of oriT [Strand]   22259..22341 [+]
Host baterium   Serratia entomophila strain AGR_Moraki_2

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -