Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103498
Name   oriT_AGR_345|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_345
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039148 (22257..22339 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_345|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2619 GenBank   WP_010895859
Name   Relaxase_MOO90_RS00135_AGR_345|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2425 GenBank   WP_234591458
Name   trbC_MOO90_RS00150_AGR_345|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2426 GenBank   WP_010895767
Name   t4cp2_MOO90_RS00345_AGR_345|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27932..56222

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO90_RS00140 (345p_00039) 24707..25030 - 324 WP_010895860 hypothetical protein -
MOO90_RS00145 (345p_00038) 25040..25576 - 537 WP_234591459 phospholipase D family protein -
MOO90_RS00150 (345p_00037) 25717..27894 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOO90_RS00155 (345p_00036) 27932..28930 - 999 WP_010895863 hypothetical protein trbB
MOO90_RS00160 (345p_00035) 28943..30253 - 1311 WP_010895864 hypothetical protein trbA
MOO90_RS00165 (345p_00034) 30391..30972 - 582 WP_010895865 hypothetical protein -
MOO90_RS00170 (345p_00033) 30969..33263 - 2295 WP_241389811 DotA/TraY family protein traY
MOO90_RS00175 (345p_00032) 33178..33741 - 564 WP_010895867 conjugal transfer protein TraX -
MOO90_RS00180 (345p_00031) 33734..34960 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO90_RS00185 (345p_00030) 35101..38175 - 3075 WP_010895869 TraU traU
MOO90_RS00190 (345p_00029) 38178..38783 - 606 WP_010895870 TraT traT
MOO90_RS00195 (345p_00028) 38869..39270 - 402 WP_010895871 DUF6750 family protein traR
MOO90_RS00200 (345p_00027) 39286..39816 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO90_RS00205 (345p_00026) 39825..40568 - 744 WP_010895873 hypothetical protein traP
MOO90_RS00210 (345p_00025) 40568..41800 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO90_RS00215 (345p_00024) 41805..42812 - 1008 WP_010895741 DotH/IcmK family type IV secretion protein traN
MOO90_RS00220 (345p_00023) 42815..43489 - 675 WP_010895742 DotI/IcmL/TraM family protein traM
MOO90_RS00225 (345p_00022) 43580..44071 - 492 WP_010895743 hypothetical protein traL
MOO90_RS00230 (345p_00021) 44081..47317 - 3237 WP_010895744 LPD7 domain-containing protein -
MOO90_RS00235 (345p_00019) 47460..47726 - 267 WP_010895745 IcmT/TraK family protein traK
MOO90_RS00240 (345p_00018) 47726..48817 - 1092 WP_336247134 plasmid transfer ATPase TraJ -
MOO90_RS00245 (345p_00016) 49072..49860 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO90_RS00250 (345p_00015) 49857..50315 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO90_RS00255 (345p_00014) 50353..51786 - 1434 WP_241390442 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO90_RS00260 (345p_00013) 51783..52445 - 663 WP_169558203 prepilin peptidase -
MOO90_RS00265 (345p_00012) 52451..52936 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO90_RS00270 (345p_00011) 52991..53584 - 594 WP_010895752 type 4 pilus major pilin -
MOO90_RS00275 (345p_00010) 53620..54732 - 1113 WP_241389818 type II secretion system F family protein -
MOO90_RS00280 (345p_00009) 54732..56222 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO90_RS00285 (345p_00008) 56228..56812 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO90_RS00290 (345p_00007) 56799..58109 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO90_RS00295 (345p_00006) 58113..59747 - 1635 WP_010895757 PilN family type IVB pilus formation outer membrane protein -
MOO90_RS00300 (345p_00005) 59772..60197 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO90_RS00305 (345p_00004) 60197..61210 - 1014 WP_010895759 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3941 GenBank   NZ_MT039148
Plasmid name   AGR_345|unnamed1 Incompatibility group   -
Plasmid size   153832 bp Coordinate of oriT [Strand]   22257..22339 [+]
Host baterium   Serratia entomophila strain AGR_345

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -