Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103496
Name   oriT_AGR_1100|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_1100
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039156 (24611..24693 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_1100|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2618 GenBank   WP_010895859
Name   Relaxase_MOO95_RS00150_AGR_1100|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2423 GenBank   WP_234591458
Name   trbC_MOO95_RS00165_AGR_1100|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2424 GenBank   WP_010895767
Name   t4cp2_MOO95_RS00360_AGR_1100|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30286..58577

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO95_RS00155 (1100p_00041) 27061..27384 - 324 WP_010895860 hypothetical protein -
MOO95_RS00160 (1100p_00042) 27394..27930 - 537 WP_234591459 phospholipase D family protein -
MOO95_RS00165 (1100p_00043) 28071..30248 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOO95_RS00170 (1100p_00044) 30286..31284 - 999 WP_010895863 hypothetical protein trbB
MOO95_RS00175 (1100p_00045) 31297..32607 - 1311 WP_241389809 conjugal transfer protein TrbA trbA
MOO95_RS00180 (1100p_00046) 32745..33326 - 582 WP_010895865 hypothetical protein -
MOO95_RS00185 (1100p_00047) 33323..35617 - 2295 WP_241389811 DotA/TraY family protein traY
MOO95_RS00190 (1100p_00048) 35532..36095 - 564 WP_010895867 conjugal transfer protein TraX -
MOO95_RS00195 (1100p_00049) 36088..37314 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO95_RS00200 (1100p_00051) 37455..40529 - 3075 WP_241389812 type IV secretory system conjugative DNA transfer family protein traU
MOO95_RS00205 (1100p_00052) 40532..41137 - 606 WP_010895870 TraT traT
MOO95_RS00210 (1100p_00053) 41223..41624 - 402 WP_010895871 DUF6750 family protein traR
MOO95_RS00215 (1100p_00054) 41640..42170 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO95_RS00220 (1100p_00055) 42179..42922 - 744 WP_241389813 hypothetical protein traP
MOO95_RS00225 (1100p_00056) 42922..44154 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO95_RS00230 (1100p_00057) 44159..45166 - 1008 WP_241389814 DotH/IcmK family type IV secretion protein traN
MOO95_RS00235 (1100p_00058) 45169..45843 - 675 WP_241389815 DotI/IcmL/TraM family protein traM
MOO95_RS00240 (1100p_00059) 45934..46425 - 492 WP_010895743 hypothetical protein traL
MOO95_RS00245 (1100p_00060) 46435..49671 - 3237 WP_241389816 LPD7 domain-containing protein -
MOO95_RS00250 (1100p_00062) 49814..50080 - 267 WP_010895745 IcmT/TraK family protein traK
MOO95_RS00255 (1100p_00063) 50080..51237 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOO95_RS00260 (1100p_00065) 51427..52215 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO95_RS00265 (1100p_00066) 52212..52670 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO95_RS00270 (1100p_00067) 52708..54141 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO95_RS00275 (1100p_00068) 54138..54806 - 669 WP_241389817 prepilin peptidase -
MOO95_RS00280 (1100p_00069) 54806..55291 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO95_RS00285 (1100p_00070) 55346..55939 - 594 WP_010895752 type 4 pilus major pilin -
MOO95_RS00290 (1100p_00071) 55975..57087 - 1113 WP_241389818 type II secretion system F family protein -
MOO95_RS00295 (1100p_00072) 57087..58577 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO95_RS00300 (1100p_00073) 58583..59167 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO95_RS00305 (1100p_00074) 59154..60464 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO95_RS00310 (1100p_00075) 60468..62102 - 1635 WP_010895757 PilN family type IVB pilus formation outer membrane protein -
MOO95_RS00315 (1100p_00076) 62127..62552 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO95_RS00320 (1100p_00077) 62552..63565 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3939 GenBank   NZ_MT039156
Plasmid name   AGR_1100|unnamed1 Incompatibility group   -
Plasmid size   154275 bp Coordinate of oriT [Strand]   24611..24693 [+]
Host baterium   Serratia entomophila strain AGR_1100

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -