Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103493
Name   oriT_AGR_626|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_626
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039155 (22259..22341 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_626|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2615 GenBank   WP_010895859
Name   Relaxase_MOO86_RS00135_AGR_626|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2418 GenBank   WP_234591458
Name   trbC_MOO86_RS00150_AGR_626|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2419 GenBank   WP_010895767
Name   t4cp2_MOO86_RS00345_AGR_626|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27934..56224

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO86_RS00140 (626p_00109) 24709..25032 - 324 WP_010895860 hypothetical protein -
MOO86_RS00145 (626p_00108) 25042..25578 - 537 WP_234591459 phospholipase D family protein -
MOO86_RS00150 (626p_00107) 25719..27896 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOO86_RS00155 (626p_00106) 27934..28932 - 999 WP_010895863 hypothetical protein trbB
MOO86_RS00160 (626p_00105) 28945..30255 - 1311 WP_241389809 conjugal transfer protein TrbA trbA
MOO86_RS00165 (626p_00104) 30393..30887 - 495 WP_241390339 hypothetical protein -
MOO86_RS00170 (626p_00103) 30971..33265 - 2295 WP_241389811 DotA/TraY family protein traY
MOO86_RS00175 (626p_00102) 33180..33743 - 564 WP_010895867 conjugal transfer protein TraX -
MOO86_RS00180 (626p_00101) 33736..34962 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO86_RS00185 (626p_00099) 35103..38177 - 3075 WP_241389812 type IV secretory system conjugative DNA transfer family protein traU
MOO86_RS00190 (626p_00098) 38180..38785 - 606 WP_010895870 TraT traT
MOO86_RS00195 (626p_00097) 38871..39272 - 402 WP_010895871 DUF6750 family protein traR
MOO86_RS00200 (626p_00096) 39288..39818 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO86_RS00205 (626p_00095) 39827..40570 - 744 WP_241389813 hypothetical protein traP
MOO86_RS00210 (626p_00094) 40570..41802 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO86_RS00215 (626p_00093) 41807..42814 - 1008 WP_241390124 DotH/IcmK family type IV secretion protein traN
MOO86_RS00220 (626p_00092) 42817..43491 - 675 WP_241389815 DotI/IcmL/TraM family protein traM
MOO86_RS00225 (626p_00091) 43582..44073 - 492 WP_010895743 hypothetical protein traL
MOO86_RS00230 (626p_00090) 44083..47319 - 3237 WP_010895744 LPD7 domain-containing protein -
MOO86_RS00235 (626p_00088) 47462..47728 - 267 WP_010895745 IcmT/TraK family protein traK
MOO86_RS00240 (626p_00087) 47728..48885 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOO86_RS00245 (626p_00085) 49075..49863 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO86_RS00250 (626p_00084) 49860..50318 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO86_RS00255 (626p_00083) 50356..51789 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO86_RS00260 (626p_00082) 51786..52454 - 669 WP_241389817 prepilin peptidase -
MOO86_RS00265 (626p_00081) 52454..52939 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO86_RS00270 (626p_00080) 52994..53461 - 468 WP_241390340 type 4 pilus major pilin -
MOO86_RS00275 (626p_00078) 53622..54737 - 1116 WP_010895753 type II secretion system F family protein -
MOO86_RS00280 (626p_00077) 54734..56224 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO86_RS00285 (626p_00076) 56230..56814 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO86_RS00290 (626p_00075) 56801..58111 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO86_RS00295 (626p_00074) 58115..59749 - 1635 WP_241390125 PilN family type IVB pilus formation outer membrane protein -
MOO86_RS00300 (626p_00073) 59774..60199 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO86_RS00305 (626p_00072) 60199..61212 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3936 GenBank   NZ_MT039155
Plasmid name   AGR_626|unnamed1 Incompatibility group   -
Plasmid size   150758 bp Coordinate of oriT [Strand]   22259..22341 [+]
Host baterium   Serratia entomophila strain AGR_626

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -