Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103490
Name   oriT_AGR_176|unnamed1 in_silico
Organism   Serratia entomophila strain AGR_176
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039144 (22584..22666 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_176|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2612 GenBank   WP_010895859
Name   Relaxase_MOO71_RS00135_AGR_176|unnamed1 insolico UniProt ID   A7M7H3
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73395.58 Da        Isoelectric Point: 7.4747

>WP_010895859.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVTECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKEHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKRPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRSGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure


Source ID Structure
AlphaFold DB A7M7H3


T4CP

ID   2413 GenBank   WP_234591458
Name   trbC_MOO71_RS00150_AGR_176|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81497.36 Da        Isoelectric Point: 8.5425

>WP_234591458.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLKRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRGVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.



ID   2414 GenBank   WP_010895767
Name   t4cp2_MOO71_RS00345_AGR_176|unnamed1 insolico UniProt ID   _
Length   577 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 577 a.a.        Molecular weight: 64155.03 Da        Isoelectric Point: 5.2094

>WP_010895767.1 MULTISPECIES: ATP-binding protein [Serratia]
MPIFNFRDEDALGKVASVDTTNVIVDVENVEQLKRLQVNHLAVLQSSRPGQHLIGLITQVTRKRGIEDIA
NDGISEQTSELNLCKIALIGTMLDRDGERENVFRRTLESVPEIDANCFSLEGQNLTNFMRTLSSVSADGN
ALTLGKYTLDEHAIAYLNGNKFFQRHAFIGGSTGSGKSWTTAKIIEQMAGLSTANAIVFDLHGEYSPLVG
KGIQHFKVAGPADVEAQRTIDNGVLYLPYWLLSYEALVAMFVDRSDQNAPNQAMIMSREINQAKRKYLED
GDHKEILKHFTVDSPVPFDLDVLMGRLNEINVEMVPGASQGKEKQGDFFGKLARMISRLENKISDRRLGF
IFSGGGDVLNFSWLEKFTTAVLGSSEENGNAGIKIINFSEVPSDVLPLIVSLVARVTFSVQQWTPSELRH
PIALLCDEAHLYMPQRNMAESADDISLDIFERIAKEGRKYGVSLIVISQRPSEVNKTMLSQCSNFVSMRL
TNAEDQGVIKRLLPDSLGGFSDILPTLDTGEALVVGDASLLPSRIRIDEPINKPNSGTVNFWDEWQKPVK
DKRLLIAVENWRKQNIQ

  Protein domains


Predicted by InterproScan.

Helicase HerA, central domain (DUF87)

(144-405)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 28259..56550

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO71_RS00140 (176p_00008) 25034..25357 - 324 WP_010895860 hypothetical protein -
MOO71_RS00145 (176p_00009) 25367..25903 - 537 WP_234591459 phospholipase D family protein -
MOO71_RS00150 (176p_00010) 26044..28221 - 2178 WP_234591458 F-type conjugative transfer protein TrbC -
MOO71_RS00155 (176p_00011) 28259..29257 - 999 WP_010895863 hypothetical protein trbB
MOO71_RS00160 (176p_00012) 29270..30580 - 1311 WP_241389809 conjugal transfer protein TrbA trbA
MOO71_RS00165 (176p_00013) 30718..31218 - 501 WP_241389810 hypothetical protein -
MOO71_RS00170 (176p_00014) 31296..33590 - 2295 WP_241389811 DotA/TraY family protein traY
MOO71_RS00175 (176p_00015) 33505..34068 - 564 WP_010895867 conjugal transfer protein TraX -
MOO71_RS00180 (176p_00016) 34061..35287 - 1227 WP_010895868 conjugal transfer protein TraW traW
MOO71_RS00185 (176p_00018) 35428..38502 - 3075 WP_241389812 type IV secretory system conjugative DNA transfer family protein traU
MOO71_RS00190 (176p_00019) 38505..39110 - 606 WP_010895870 TraT traT
MOO71_RS00195 (176p_00020) 39196..39597 - 402 WP_010895871 DUF6750 family protein traR
MOO71_RS00200 (176p_00021) 39613..40143 - 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO71_RS00205 (176p_00022) 40152..40895 - 744 WP_241389813 hypothetical protein traP
MOO71_RS00210 (176p_00023) 40895..42127 - 1233 WP_010895874 conjugal transfer protein TraO traO
MOO71_RS00215 (176p_00024) 42132..43139 - 1008 WP_241390124 DotH/IcmK family type IV secretion protein traN
MOO71_RS00220 (176p_00025) 43142..43816 - 675 WP_241389815 DotI/IcmL/TraM family protein traM
MOO71_RS00225 (176p_00026) 43907..44398 - 492 WP_010895743 hypothetical protein traL
MOO71_RS00230 (176p_00027) 44408..47644 - 3237 WP_010895744 LPD7 domain-containing protein -
MOO71_RS00235 (176p_00029) 47787..48053 - 267 WP_010895745 IcmT/TraK family protein traK
MOO71_RS00240 (176p_00030) 48053..49210 - 1158 WP_010895746 plasmid transfer ATPase TraJ virB11
MOO71_RS00245 (176p_00032) 49400..50188 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOO71_RS00250 (176p_00033) 50185..50643 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOO71_RS00255 (176p_00034) 50681..52114 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOO71_RS00260 (176p_00035) 52111..52779 - 669 WP_241389817 prepilin peptidase -
MOO71_RS00265 (176p_00036) 52779..53264 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOO71_RS00270 (176p_00037) 53319..53912 - 594 WP_010895752 type 4 pilus major pilin -
MOO71_RS00275 (176p_00038) 53948..55060 - 1113 WP_241389818 type II secretion system F family protein -
MOO71_RS00280 (176p_00039) 55060..56550 - 1491 WP_010895754 ATPase, T2SS/T4P/T4SS family virB11
MOO71_RS00285 (176p_00040) 56556..57140 - 585 WP_010895755 type IV pilus biogenesis protein PilP -
MOO71_RS00290 (176p_00041) 57127..58437 - 1311 WP_010895756 type 4b pilus protein PilO2 -
MOO71_RS00295 (176p_00042) 58441..60075 - 1635 WP_241390125 PilN family type IVB pilus formation outer membrane protein -
MOO71_RS00300 (176p_00043) 60100..60525 - 426 WP_010895758 type IV pilus biogenesis protein PilM -
MOO71_RS00305 (176p_00044) 60525..61538 - 1014 WP_241389819 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3933 GenBank   NZ_MT039144
Plasmid name   AGR_176|unnamed1 Incompatibility group   -
Plasmid size   150171 bp Coordinate of oriT [Strand]   22584..22666 [+]
Host baterium   Serratia entomophila strain AGR_176

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -