Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103489
Name   oriT_AGR_348|unnamed1 in_silico
Organism   Serratia grimesii strain AGR_348
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039164 (26044..26125 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_AGR_348|unnamed1
CGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2611 GenBank   WP_241390007
Name   Relaxase_MOO72_RS00115_AGR_348|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73413.65 Da        Isoelectric Point: 7.3733

>WP_241390007.1 TraI/MobA(P) family conjugative relaxase [Serratia grimesii]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDTPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNITTASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKSLTPSGAKALEYYADVESLHTYAVTECGFKIDEAMADPNLTWKDVHRILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLGCLEKDLGLFQPLENVGTYTKDHTTTATDAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKHTWKRPRLDASVVKRRYQNESKRFAWQKARARVAVGDP
LLRKLTYHIIEVERMKAMAALRLAVKEERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPISTNSIVCAVADDTPAFALEGYATQVTRDGTVQYVRDGCVELQDKGERIEVGNHRVNEGEHIA
GGMALAENKSGEHLKFEGESAFVQQACSLVRWFNEGGDTPLPLSDPQQRILAGYDASSQAISTGEGQHKL
SAAEQPDVEQSKPRSGYRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(519-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-318)


  Protein structure



No available structure.




T4CP

ID   2412 GenBank   WP_262190542
Name   trbC_MOO72_RS00100_AGR_348|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81720.52 Da        Isoelectric Point: 8.5434

>WP_262190542.1 F-type conjugative transfer protein TrbC [Serratia grimesii]
MSRPVEVDPRRVRRPLGYTFINDALLSPIGVQLSLLAGLIVGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELIKKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFDLARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSSLPMRINRFIDVETPTEAELFTLNPSLKRKLPPTAQEIDG
ILQRLEQAMDTTTSAGMMDPVLKRVAAVALDLDNRADISYSPTQRGVLLFEAAREALHQNKRNWRQLPSP
PKPIRVSKEVAQSLANASTNQITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 783..20577

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO72_RS00005 (348p1_00001) 1..783 + 783 WP_336251214 plasmid transfer ATPase TraJ -
MOO72_RS00010 (348p1_00002) 783..1049 + 267 WP_241389991 IcmT/TraK family protein traK
MOO72_RS00015 (348p1_00004) 1192..1530 + 339 WP_241389992 DUF5710 domain-containing protein -
MOO72_RS00020 (348p1_00005) 1488..4424 + 2937 WP_241389993 LPD7 domain-containing protein -
MOO72_RS00025 (348p1_00006) 4434..4925 + 492 WP_241389994 hypothetical protein traL
MOO72_RS00030 (348p1_00007) 4949..5692 + 744 WP_241389995 DotI/IcmL/TraM family protein traM
MOO72_RS00035 (348p1_00009) 5946..6701 + 756 WP_241390046 DotH/IcmK family type IV secretion protein traN
MOO72_RS00040 (348p1_00010) 6706..7938 + 1233 WP_127147003 conjugal transfer protein TraO traO
MOO72_RS00045 (348p1_00011) 7938..8681 + 744 WP_241389996 hypothetical protein traP
MOO72_RS00050 (348p1_00012) 8690..9220 + 531 WP_010895872 conjugal transfer protein TraQ traQ
MOO72_RS00055 (348p1_00013) 9236..9637 + 402 WP_241389997 DUF6750 family protein traR
MOO72_RS00060 (348p1_00014) 9722..10327 + 606 WP_241389998 hypothetical protein traT
MOO72_RS00065 (348p1_00015) 10330..13404 + 3075 WP_241389999 type IV secretory system conjugative DNA transfer family protein traU
MOO72_RS00070 (348p1_00017) 13545..14771 + 1227 WP_241390000 conjugal transfer protein TraW traW
MOO72_RS00075 (348p1_00018) 14764..15327 + 564 WP_241390001 conjugal transfer protein TraX -
MOO72_RS00080 (348p1_00019) 15260..17536 + 2277 WP_336251215 DotA/TraY family protein -
MOO72_RS00085 (348p1_00020) 17533..18111 + 579 WP_241390002 hypothetical protein -
MOO72_RS00090 (348p1_00021) 18291..19550 + 1260 WP_241390003 conjugal transfer protein TrbA trbA
MOO72_RS00095 (348p1_00022) 19564..20577 + 1014 WP_241390004 hypothetical protein trbB
MOO72_RS00100 (348p1_00023) 20615..22792 + 2178 WP_262190542 F-type conjugative transfer protein TrbC -
MOO72_RS00105 (348p1_00024) 22803..23339 + 537 WP_241390006 phospholipase D family protein -
MOO72_RS00110 (348p1_00025) 23349..23672 + 324 WP_127147014 hypothetical protein -

Host bacterium

ID   3932 GenBank   NZ_MT039164
Plasmid name   AGR_348|unnamed1 Incompatibility group   -
Plasmid size   149773 bp Coordinate of oriT [Strand]   26044..26125 [-]
Host baterium   Serratia grimesii strain AGR_348

Cargo genes

Drug resistance gene   -
Virulence gene   pilW
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7