Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 103481 |
| Name | oriT_AGR_442|unnamed1  |
| Organism | Serratia entomophila strain AGR_442 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MT039154 (1241..1832 [-], 592 nt) |
| oriT length | 592 nt |
| IRs (inverted repeats) | 566..571, 576..581 (ATGCCC..GGGCAT) 494..500, 505..511 (CCTCCCG..CGGGAGG) 430..435, 439..444 (GTTCGC..GCGAAC) 72..77, 83..88 (TGCTTT..AAAGCA) 67..72, 83..88 (TGCTTT..AAAGCA) |
| Location of nic site | 291..292 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 592 nt
>oriT_AGR_442|unnamed1
TGTTAAATACATTGTGGTTTTGATAAAATCATTAGTATCTGGAATAACGTCATCATCATCTTCTACTGCTTTGCTTTTAAGTAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCAGGTTCATCTGGATAAATAACTTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCCAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCATTGATCTAGCACTACTCTTGAATTGTGCTTCAGCGTCGAGAAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACTGGAAACATCTCAATCGCGGTGGGGTGTGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTAATGACGTGTGAATCTATTCAGGGTCTGGTGTGTCGCCTCCCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTGGTTTTGATAAAATCATTAGTATCTGGAATAACGTCATCATCATCTTCTACTGCTTTGCTTTTAAGTAAAGCACTGGCAAGCAACTTCATGCCTTTAGCAACAGTTAAACCAGGTTCATCTGGATAAATAACTTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCCAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCATTGATCTAGCACTACTCTTGAATTGTGCTTCAGCGTCGAGAAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACTGGAAACATCTCAATCGCGGTGGGGTGTGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTAATGACGTGTGAATCTATTCAGGGTCTGGTGTGTCGCCTCCCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 2604 | GenBank | WP_241389438 |
| Name | Relaxase_MOO57_RS00030_AGR_442|unnamed1 |
UniProt ID | _ |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44399.82 Da Isoelectric Point: 10.6126
>WP_241389438.1 MobP1 family relaxase [Serratia entomophila]
MGVSVDKEYRVKRKSSESGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQHVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGTDF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWKLDNLGVKGIDRNPSSSKELVLN
SAAMLQKQQLQMKQFTQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRVKRKSSESGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQHVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGTDF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWKLDNLGVKGIDRNPSSSKELVLN
SAAMLQKQQLQMKQFTQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 2404 | GenBank | WP_241389447 |
| Name | t4cp2_MOO57_RS00090_AGR_442|unnamed1 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69131.78 Da Isoelectric Point: 6.9118
>WP_241389447.1 type IV secretory system conjugative DNA transfer family protein [Serratia entomophila]
MSLKLPDKGQWLFIGFVMCLSTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTTSIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWLFIGFVMCLSTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTTSIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4402..17214
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MOO57_RS00005 (442p_00001) | 120..359 | + | 240 | WP_044715143 | hypothetical protein | - |
| MOO57_RS00010 (442p_00002) | 456..797 | + | 342 | WP_241389436 | hypothetical protein | - |
| MOO57_RS00015 (442p_00003) | 873..1022 | + | 150 | WP_162852120 | hypothetical protein | - |
| MOO57_RS00020 (442p_00004) | 1038..1295 | + | 258 | WP_015256854 | hypothetical protein | - |
| MOO57_RS00025 (442p_00006) | 1623..2168 | + | 546 | WP_261243033 | DNA distortion polypeptide 1 | - |
| MOO57_RS00030 (442p_00007) | 2171..3331 | + | 1161 | WP_241389438 | MobP1 family relaxase | - |
| MOO57_RS00035 (442p_00008) | 3687..4178 | + | 492 | WP_241389439 | transcription termination/antitermination NusG family protein | - |
| MOO57_RS00040 (442p_00010) | 4402..5046 | + | 645 | WP_241389440 | lytic transglycosylase domain-containing protein | virB1 |
| MOO57_RS00045 (442p_00011) | 5030..5320 | + | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| MOO57_RS00050 (442p_00012) | 5342..8101 | + | 2760 | WP_241389441 | VirB3 family type IV secretion system protein | virb4 |
| MOO57_RS00055 (442p_00013) | 8112..8858 | + | 747 | WP_241389453 | type IV secretion system protein | - |
| MOO57_RS00060 (442p_00014) | 8868..9125 | + | 258 | WP_241389442 | EexN family lipoprotein | - |
| MOO57_RS00065 (442p_00015) | 9137..10192 | + | 1056 | WP_241389443 | type IV secretion system protein | virB6 |
| MOO57_RS00070 (442p_00016) | 10432..11160 | + | 729 | WP_241389444 | type IV secretion system protein | virB8 |
| MOO57_RS00075 (442p_00017) | 11165..12094 | + | 930 | WP_241389445 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| MOO57_RS00080 (442p_00018) | 12091..13302 | + | 1212 | WP_241389446 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| MOO57_RS00085 (442p_00019) | 13326..14357 | + | 1032 | WP_241389454 | P-type DNA transfer ATPase VirB11 | virB11 |
| MOO57_RS00090 (442p_00020) | 14360..16195 | + | 1836 | WP_241389447 | type IV secretory system conjugative DNA transfer family protein | - |
| MOO57_RS00095 (442p_00021) | 16192..16590 | + | 399 | WP_241389448 | cag pathogenicity island Cag12 family protein | - |
| MOO57_RS00100 (442p_00022) | 16595..16933 | + | 339 | WP_241389449 | hypothetical protein | - |
| MOO57_RS00105 (442p_00023) | 17002..17214 | + | 213 | WP_241389450 | TraM recognition domain-containing protein | virb4 |
| MOO57_RS00110 (442p_00024) | 17214..17540 | + | 327 | WP_241389451 | hypothetical protein | - |
| MOO57_RS00115 (442p_00025) | 17534..17968 | + | 435 | WP_241389452 | hypothetical protein | - |
| MOO57_RS00120 (442p_00026) | 17965..18435 | + | 471 | WP_241389418 | thermonuclease family protein | - |
| MOO57_RS00125 (442p_00027) | 18447..18806 | + | 360 | WP_241389419 | G-protein coupled receptor | - |
| MOO57_RS00130 (442p_00028) | 18810..19583 | + | 774 | WP_241389420 | hypothetical protein | - |
| MOO57_RS00135 (442p_00029) | 19595..20251 | + | 657 | WP_241389421 | hypothetical protein | - |
| MOO57_RS00140 (442p_00030) | 20254..20910 | + | 657 | WP_241389422 | hypothetical protein | - |
Host bacterium
| ID | 3924 | GenBank | NZ_MT039154 |
| Plasmid name | AGR_442|unnamed1 | Incompatibility group | IncX5 |
| Plasmid size | 35134 bp | Coordinate of oriT [Strand] | 1241..1832 [-] |
| Host baterium | Serratia entomophila strain AGR_442 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |