Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103480
Name   oriT_p77_84_18 in_silico
Organism   Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain 77_84_18
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ666126 (20002..20255 [-], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_p77_84_18
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2603 GenBank   WP_049589872
Name   TraI_2_LQA00_RS00110_p77_84_18 insolico UniProt ID   A0A5X7KNX7
Length   1048 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1048 a.a.        Molecular weight: 117787.06 Da        Isoelectric Point: 4.6054

>WP_049589872.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPTGI
QTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLL
SHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPS
SQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYTDG
NDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGEVY
LNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDTVE
TAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDE
SAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGSLD
GMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELP
PPRVNALEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVGSC
ATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAAT
SVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPV
SGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTLYL
TQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-302)


  Protein structure


Source ID Structure
AlphaFold DB A0A5X7KNX7


T4CP

ID   2403 GenBank   WP_001284076
Name   traD_LQA00_RS00115_p77_84_18 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 91753..100960

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQA00_RS00475 86862..87578 - 717 WP_000838218 zinc finger-like domain-containing protein -
LQA00_RS00480 87575..87775 - 201 WP_000594931 hypothetical protein -
LQA00_RS00485 87793..88848 - 1056 WP_001065779 hypothetical protein -
LQA00_RS00490 89325..90200 + 876 WP_000594612 RepB family plasmid replication initiator protein -
LQA00_RS00495 91349..91702 + 354 WP_000423602 hypothetical protein -
LQA00_RS00500 91753..92070 + 318 WP_000043357 type IV conjugative transfer system protein TraL traL
LQA00_RS00505 92082..92870 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
LQA00_RS00510 92870..94141 + 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
LQA00_RS00515 94143..94604 + 462 WP_000521240 plasmid transfer protein HtdO -
LQA00_RS00520 94594..95949 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
LQA00_RS00525 95957..96439 + 483 WP_000377633 hypothetical protein -
LQA00_RS00530 96558..97310 + 753 WP_183079335 protein-disulfide isomerase HtdT -
LQA00_RS00535 97320..98270 + 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
LQA00_RS00540 98279..100960 + 2682 WP_000387412 TraC family protein virb4
LQA00_RS00545 103803..105056 + 1254 WP_032084678 ParA family protein -

Host bacterium

ID   3923 GenBank   NZ_MZ666126
Plasmid name   p77_84_18 Incompatibility group   IncHI2
Plasmid size   227392 bp Coordinate of oriT [Strand]   20002..20255 [-]
Host baterium   Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain 77_84_18

Cargo genes

Drug resistance gene   mcr-1.1, tet(M), blaTEM-1A, sul2, aph(3'')-Ib, aph(6)-Id, aac(3)-IVa, aph(4)-Ia, mcr-5.1
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -