Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   103479
Name   oriT_AGR_1770|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_1770
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039199 (22628..22710 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_1770|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   2602 GenBank   WP_241390305
Name   Relaxase_MOP31_RS00140_AGR_1770|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73396.69 Da        Isoelectric Point: 7.3282

>WP_241390305.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Serratia]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSECGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)


  Protein structure



No available structure.




T4CP

ID   2402 GenBank   WP_241390158
Name   trbC_MOP31_RS00180_AGR_1770|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33508..61787

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP31_RS00170 (1770p1_00044) 29717..30661 + 945 WP_241389593 hypothetical protein -
MOP31_RS00175 (1770p1_00045) 30797..31282 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP31_RS00180 (1770p1_00046) 31293..33470 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP31_RS00185 (1770p1_00047) 33508..34506 - 999 WP_241390308 hypothetical protein trbB
MOP31_RS00190 (1770p1_00048) 34519..35742 - 1224 WP_241391019 conjugal transfer protein TrbA trbA
MOP31_RS00195 (1770p1_00049) 35958..36536 - 579 WP_241389923 hypothetical protein -
MOP31_RS00200 (1770p1_00050) 36533..38827 - 2295 WP_261097221 DotA/TraY family protein -
MOP31_RS00205 (1770p1_00051) 38742..39305 - 564 WP_241390311 conjugal transfer protein TraX -
MOP31_RS00210 (1770p1_00052) 39298..40524 - 1227 WP_241390312 conjugal transfer protein TraW traW
MOP31_RS00215 (1770p1_00054) 40665..43739 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP31_RS00220 (1770p1_00055) 43742..44347 - 606 WP_241389875 hypothetical protein traT
MOP31_RS00225 (1770p1_00056) 44434..44835 - 402 WP_241389604 DUF6750 family protein traR
MOP31_RS00230 (1770p1_00057) 44851..45381 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP31_RS00235 (1770p1_00058) 45390..46133 - 744 WP_241390314 hypothetical protein traP
MOP31_RS00240 (1770p1_00059) 46133..47365 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOP31_RS00245 (1770p1_00060) 47369..48376 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP31_RS00250 (1770p1_00061) 48379..49053 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP31_RS00255 (1770p1_00062) 49144..49635 - 492 WP_010895743 hypothetical protein traL
MOP31_RS00260 (1770p1_00063) 49645..52881 - 3237 WP_241390318 LPD7 domain-containing protein -
MOP31_RS00265 (1770p1_00065) 53024..53290 - 267 WP_241390319 IcmT/TraK family protein traK
MOP31_RS00270 (1770p1_00066) 53290..54447 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP31_RS00275 (1770p1_00068) 54637..55425 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP31_RS00280 (1770p1_00069) 55422..55880 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP31_RS00285 (1770p1_00070) 55918..57351 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP31_RS00290 (1770p1_00071) 57348..58016 - 669 WP_241390170 prepilin peptidase -
MOP31_RS00295 (1770p1_00072) 58016..58501 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP31_RS00300 (1770p1_00073) 58556..59149 - 594 WP_010895752 type 4 pilus major pilin -
MOP31_RS00305 (1770p1_00074) 59185..60297 - 1113 WP_241389818 type II secretion system F family protein -
MOP31_RS00310 (1770p1_00075) 60297..61787 - 1491 WP_241389888 ATPase, T2SS/T4P/T4SS family virB11
MOP31_RS00315 (1770p1_00076) 61793..62377 - 585 WP_241389889 type IV pilus biogenesis protein PilP -
MOP31_RS00320 (1770p1_00077) 62364..63674 - 1311 WP_241390279 type 4b pilus protein PilO2 -
MOP31_RS00325 (1770p1_00078) 63678..65312 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP31_RS00330 (1770p1_00079) 65334..65759 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP31_RS00335 (1770p1_00080) 65759..66772 - 1014 WP_241390280 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   3922 GenBank   NZ_MT039199
Plasmid name   AGR_1770|unnamed1 Incompatibility group   -
Plasmid size   144458 bp Coordinate of oriT [Strand]   22628..22710 [+]
Host baterium   Serratia proteamaculans strain AGR_1770

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -